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Background: Chitosan (CS) and tripolyphosphate (TPP) can be combined in the development of a material with synergistic properties and promising potential for the conservation of food products. In this study, ellagic acid (EA) and anti-inflammatory peptide (FPL)-loaded CS nanoparticles (FPL/EA NPs) were prepared using the ionic gelation method and optimal preparation conditions were obtained through a single factor design., Results: The synthesized nanoparticles (NPs) were characterized using a scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC). Nanoparticles were spherical, with an average size of 308.33 ± 4.61 nm, a polydispersity index (PDI) of 0.254, a zeta potential of +31.7 ± 0.08 mV, and a high encapsulation capacity (22.16 ± 0.79%). An in vitro release study showed that EA/FPL had a sustainable release from FPL/EA NPs. The stability of the FPL/EA NPs was evaluated for 90 days at 0, 25, and 37 °C. Significant anti-inflammatory activity of FPL/EA NPs was verified by nitric oxide (NO) and tumor necrosis factor-α (TNF-α) reduction., Conclusion: These characteristics support the use of CS nanoparticles to encapsulate EA and FPL and improve their bioactivity in food products. © 2023 Society of Chemical Industry., (© 2023 Society of Chemical Industry.)

Selenium nanoparticles (SeNPs) have attracted lots of attention recently owing to their excellent bioavailability and low toxicity. However, the stability of SeNPs needs to be improved. To enhance the stability of SeNPs, we used Spirulina platensis polysaccharides (SPs) as stabilizers to prepare SPs-SeNPs. The SPs-SeNPs were spherical, with a mean particle size of 73.42 ± 0.69 nm. The optimal preparation conditions for the SPs-SeNPs were a SPs concentration of 100 mg/L, ascorbic acid/sodium selenite concentration ratio of 3:1 and reaction time of 6 hr. The prepared SPs-SeNPs were stable for 75 d at 4°C. Furthermore, MTT assay showed that the median toxic concentration (TC 50 ) of SPs-SeNPs was approximately 22,000 μg/L. In contrast, the TC 50 value of selenite was approximately 400 μg/L, which confirmed that the cytotoxicity of SPs-SeNPs on RAW264.7 cells was significantly reduced compared with sodium selenite. In conclusion, SPs can improve the stability of SeNPs. The toxicity of SPs-SeNPs is lower than that of sodium selenite. PRACTICAL APPLICATIONS: Spirulina platensis polysaccharides can improve the stability and reduce the toxicity of selenium nanoparticles. It can lay the foundation for the comprehensive utilization of S. platensis and the development and research of polysaccharide nano-selenium complexes., (© 2020 Wiley Periodicals LLC.)