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Your search keyword '"Praveena Jayaraman"' showing total 2 results
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2 results on '"Praveena Jayaraman"'

1. Minocycline Loaded Hybrid Composites Nanoparticles for Mesenchymal Stem Cells Differentiation into Osteogenesis.

Author

Tham AY, Gandhimathi C, Praveena J, Venugopal JR, Ramakrishna S, and Kumar SD

Subjects
Animals, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents pharmacology, Cell Adhesion drug effects, Cells, Cultured, Extracellular Matrix drug effects, Fibroins pharmacology, Fluoresceins chemistry, Fluorescent Dyes chemistry, Humans, Mesenchymal Stem Cells drug effects, Mice, Minocycline administration & dosage, NIH 3T3 Cells, Polyesters pharmacology, Silk chemistry, Tissue Engineering, Tissue Scaffolds, Cell Differentiation drug effects, Cell Proliferation drug effects, Mesenchymal Stem Cells cytology, Minocycline pharmacology, Nanoparticles administration & dosage, Osteogenesis drug effects
Abstract

Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering.

Published
2016
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2. Controlled release of drugs in electrosprayed nanoparticles for bone tissue engineering

Author

Dinesh Kumar Srinivasan, Jayarama Reddy Venugopal, Seeram Ramakrishna, David Laurence Becker, Praveena Jayaraman, Chinnasamy Gandhimathi, and Lee Kong Chian School of Medicine (LKCMedicine)

Subjects
Bone Regeneration, Polymers, Surface Properties, Pharmaceutical Science, Biocompatible Materials, Nanotechnology, Bone tissue, Regenerative medicine, Drug Delivery Systems, Tissue engineering, Bone cell, medicine, Humans, Technology, Pharmaceutical, Particle Size, Bone regeneration, Osteoblasts, Tissue Engineering, Tissue Scaffolds, Chemistry, Mesenchymal Stem Cells, Controlled release, Anti-Bacterial Agents, Electrospray, nanoparticles, drug delivery systems, bone tissue, regenerative medicine, medicine.anatomical_structure, Delayed-Action Preparations, Drug delivery, Nanoparticles, Nanomedicine, Porosity
Abstract

Generating porous topographic substrates, by mimicking the native extracellular matrix (ECM) to promote the regeneration of damaged bone tissues, is a challenging process. Generally, scaffolds developed for bone tissue regeneration support bone cell growth and induce bone-forming cells by natural proteins and growth factors. Limitations are often associated with these approaches such as improper scaffold stability, and insufficient cell adhesion, proliferation, differentiation, and mineralization with less growth factor expression. Therefore, the use of engineered nanoparticles has been rapidly increasing in bone tissue engineering (BTE) applications. The electrospray technique is advantageous over other conventional methods as it generates nanomaterials of particle sizes in the micro/nanoscale range. The size and charge of the particles are controlled by regulating the polymer solution flow rate and electric voltage. The unique properties of nanoparticles such as large surface area-to-volume ratio, small size, and higher reactivity make them promising candidates in the field of biomedical engineering. These nanomaterials are extensively used as therapeutic agents and for drug delivery, mimicking ECM, and restoring and improving the functions of damaged organs. The controlled and sustained release of encapsulated drugs, proteins, vaccines, growth factors, cells, and nucleotides from nanoparticles has been well developed in nanomedicine. This review provides an insight into the preparation of nanoparticles by electrospraying technique and illustrates the use of nanoparticles in drug delivery for promoting bone tissue regeneration. MOE (Min. of Education, S’pore) Accepted version

Published
2015
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