1. Nanoparticle-mediated combination chemotherapy and photodynamic therapy overcomes tumor drug resistance.
- Author
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Khdair A, Chen D, Patil Y, Ma L, Dou QP, Shekhar MP, and Panyam J
- Subjects
- Adenocarcinoma blood supply, Adenocarcinoma metabolism, Adenocarcinoma pathology, Alginates chemistry, Animals, Antibiotics, Antineoplastic administration & dosage, Antibiotics, Antineoplastic chemistry, Antibiotics, Antineoplastic metabolism, Apoptosis drug effects, Biological Transport, Cell Proliferation drug effects, Chemistry, Pharmaceutical, Dioctyl Sulfosuccinic Acid chemistry, Doxorubicin administration & dosage, Doxorubicin chemistry, Doxorubicin metabolism, Drug Compounding, Female, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Mammary Neoplasms, Experimental blood supply, Mammary Neoplasms, Experimental metabolism, Mammary Neoplasms, Experimental pathology, Methylene Blue administration & dosage, Methylene Blue chemistry, Methylene Blue metabolism, Mice, Mice, Inbred BALB C, Microvessels drug effects, Microvessels pathology, Time Factors, Adenocarcinoma drug therapy, Antibiotics, Antineoplastic pharmacology, Doxorubicin pharmacology, Drug Carriers, Drug Resistance, Neoplasm, Mammary Neoplasms, Experimental drug therapy, Methylene Blue pharmacology, Nanoparticles, Photochemotherapy
- Abstract
Tumor drug resistance significantly limits the success of chemotherapy in the clinic. Tumor cells utilize multiple mechanisms to prevent the accumulation of anticancer drugs at their intracellular site of action. In this study, we investigated the anticancer efficacy of doxorubicin in combination with photodynamic therapy using methylene blue in a drug-resistant mouse tumor model. Surfactant-polymer hybrid nanoparticles formulated using an anionic surfactant, Aerosol-OT (AOT), and a naturally occurring polysaccharide polymer, sodium alginate, were used for synchronized delivery of the two drugs. Balb/c mice bearing syngeneic JC tumors (mammary adenocarcinoma) were used as a drug-resistant tumor model. Nanoparticle-mediated combination therapy significantly inhibited tumor growth and improved animal survival. Nanoparticle-mediated combination treatment resulted in enhanced tumor accumulation of both doxorubicin and methylene blue, significant inhibition of tumor cell proliferation, and increased induction of apoptosis. These data suggest that nanoparticle-mediated combination chemotherapy and photodynamic therapy using doxorubicin and methylene blue has significant therapeutic potential against drug-resistant tumors., (Copyright 2009 Elsevier B.V. All rights reserved.)
- Published
- 2010
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