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3 results on '"Ezhuthupurakkal, Preedia Babu"'

1. Anticancer potential of ZnO nanoparticle-ferulic acid conjugate on Huh-7 and HepG2 cells and diethyl nitrosamine induced hepatocellular cancer on Wistar albino rat.

Author

Ezhuthupurakkal PB, Ariraman S, Arumugam S, Subramaniyan N, Muthuvel SK, Kumpati P, Rajamani B, and Chinnasamy T

Subjects
Alkylating Agents toxicity, Animals, Antineoplastic Agents chemistry, Apoptosis drug effects, Carcinoma, Hepatocellular pathology, Cell Proliferation drug effects, Hep G2 Cells, Humans, Liver Neoplasms, Experimental chemically induced, Liver Neoplasms, Experimental pathology, Nanoparticles chemistry, Rats, Rats, Wistar, Tumor Cells, Cultured, Antineoplastic Agents administration & dosage, Carcinoma, Hepatocellular drug therapy, Coumaric Acids chemistry, Diethylnitrosamine toxicity, Liver Neoplasms, Experimental drug therapy, Nanoparticles administration & dosage, Zinc Oxide chemistry
Abstract

Drawbacks and limitations of recently available therapies to hepatocellular cancer (HCC) devoted the scientist to focus on emerging new strategies. ZnO nanoparticles (ZnONPs) based chemotherapeutics has been emanating as a promising approach to maximize therapeutic synergy facilitating the discovery of novel multitargeted combinations. In the present study we conjugated ZnONPs with ferulic acid (ZnONPs-FAC) characterized by computational, spectroscopic and microscopic techniques. In vitro anticancer potential has been evaluated by assessing cell viability, morphology, ROS generation, mitochondrial membrane permeability, comet assay, immunofluorescent staining of 8-OHdG, Ki67 and γ-H2AX, cell cycle analysis and western blot analysis and in vivo anticancer potential against DEN induced HCC was analyzed by histopathological and immunohistochemical methods. The results revealed that ZnONPs-FAC induces cell death through apoptosis and can suppress the DEN-induced HCC. Our study documents therapeutic potential of nanoparticle conjugated with phytochemicals, suggesting a new platform for combinatorial chemotherapy., (Copyright © 2017 Elsevier Inc. All rights reserved.)

Published
2018
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2. Synthesis and characterisation of arsenic nanoparticles and its interaction with DNA and cytotoxic potential on breast cancer cells

Author

A. Subastri, Preeti Sharma, Ghedeir M. Alshammari, Chinnasamy Thirunavukkarasu, Subramaniyam Nithyananthan, Viswanathan Arun, Balakrishnan Aristatile, Venkataraman Dharuman, Ezhuthupurakkal Preedia babu, and A. Suyavaran

Subjects
Surface Properties, Nanoparticle, Antineoplastic Agents, Breast Neoplasms, 02 engineering and technology, 010402 general chemistry, Toxicology, 01 natural sciences, Structure-Activity Relationship, chemistry.chemical_compound, Arsenic Trioxide, Humans, Cytotoxic T cell, Particle Size, Arsenic trioxide, Cytotoxicity, Cells, Cultured, Cell Proliferation, Dose-Response Relationship, Drug, Acridine orange, DNA, Neoplasm, General Medicine, 021001 nanoscience & nanotechnology, Binding constant, 0104 chemical sciences, Comet assay, HEK293 Cells, chemistry, Cancer research, Nanoparticles, Female, Drug Screening Assays, Antitumor, 0210 nano-technology, DNA
Abstract

Therapeutic applications of arsenic trioxide (ATO) are limited due to their severe adverse effects. However, nanoparticles of ATO might possess inimitable biologic effects based on their structure and size which differ from their parent molecules. Based on this conception, AsNPs were synthesized from ATO and comparatively analysed for their interaction mechanism with DNA using spectroscopic & electrochemical techniques. Finally, anti-proliferative activity was assessed against different breast cancer cells (MDA-MB-231 & MCF-7) and normal non-cancerous cells (HEK-293). The DNA interaction study revealed that AsNPs and ATO exhibit binding constant values in the order of 106 which indicates strong binding interaction. Binding of AsNPs did not disturb the structural integrity of DNA, on the other hand an opposing effect was observed with ATO through biophysical techniques. Further, in vitro study, confirms cytotoxicity of ATO and AsNPs against different cells, however at particular concentration ATO exhibits more cytotoxicity than that of AsNPs. Furthermore, cytotoxicity was confirmed through acridine orange and comet assay. In conclusion, AsNPs are safer than ATO with comparable efficacy and might be a suitable candidate for the development of novel therapeutic agent against breast cancer and other solid tumours.

Published
2018
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3. Anticancer potential of ZnO nanoparticle-ferulic acid conjugate on Huh-7 and HepG2 cells and diethyl nitrosamine induced hepatocellular cancer on Wistar albino rat.

Author

Ezhuthupurakkal, Preedia Babu, Ariraman, Subastri, Arumugam, Suyavaran, Subramaniyan, Nithyananthan, Muthuvel, Suresh Kumar, Kumpati, Premkumar, Rajamani, Bharathidasan, and Chinnasamy, Thirunavukkarasu

Subjects
LIVER cancer, ZINC oxide, NANOPARTICLES, CANCER chemotherapy, FERULIC acid, ANTINEOPLASTIC agents, PHYTOCHEMICALS, THERAPEUTICS
Abstract

Drawbacks and limitations of recently available therapies to hepatocellular cancer (HCC) devoted the scientist to focus on emerging new strategies. ZnO nanoparticles (ZnONPs) based chemotherapeutics has been emanating as a promising approach to maximize therapeutic synergy facilitating the discovery of novel multitargeted combinations. In the present study we conjugated ZnONPs with ferulic acid (ZnONPs-FAC) characterized by computational, spectroscopic and microscopic techniques. In vitro anticancer potential has been evaluated by assessing cell viability, morphology, ROS generation, mitochondrial membrane permeability, comet assay, immunofluorescent staining of 8-OHdG, Ki67 and γ-H2AX, cell cycle analysis and western blot analysis and in vivo anticancer potential against DEN induced HCC was analyzed by histopathological and immunohistochemical methods. The results revealed that ZnONPs-FAC induces cell death through apoptosis and can suppress the DEN-induced HCC. Our study documents therapeutic potential of nanoparticle conjugated with phytochemicals, suggesting a new platform for combinatorial chemotherapy. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
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