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2 results on '"Claire Bankier"'

1. Nanoparticle Lysis of Cryptosporidium Oocysts

Author

Ameya Vaidya, Claire Bankier, Helinor Johnston, and Helen Bridle

Subjects
nanoparticles, Cryptosporidium, lysis, DNA extraction, Biology (General), QH301-705.5
Abstract

The extraction of DNA from Cryptosporidium oocysts is challenging due to the robust oocyst wall. Nanoparticles have been applied to disinfect Cryptosporidium oocysts; here, we demonstrate the utilisation of nanoparticles to disrupt the oocyst wall to enable sporozoite lysis and detection via PCR. Both silver and zinc oxide nanoparticles are investigated under different conditions and compared to existing techniques. Zinc oxide nanoparticles are shown to be as effective as freeze–thaw methods, suggesting that a nanoparticle lysis approach offers a viable alternative to existing methods.

Published
2024
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2. Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations

Author

Rupy Kaur Matharu, Claire Bankier, Guogang Ren, Yuen-Ki Cheong, Elaine Cloutman-Green, and Lena Ciric

Subjects
Staphylococcus aureus, lcsh:Medicine, Metal Nanoparticles, Nanoparticle, chemistry.chemical_element, Nanotechnology, Bacterial population, Microbial Sensitivity Tests, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Metal, Antibiotics, lcsh:Science, Metal nanoparticles, Multidisciplinary, Chemistry, lcsh:R, 021001 nanoscience & nanotechnology, Antimicrobial, Copper, Anti-Bacterial Agents, 3. Good health, 0104 chemical sciences, Metals, visual_art, Pseudomonas aeruginosa, visual_art.visual_art_medium, Nanoparticles, lcsh:Q, 0210 nano-technology
Abstract

Metallic nanoparticles have unique antimicrobial properties that make them suitable for use within medical and pharmaceutical devices to prevent the spread of infection in healthcare. The use of nanoparticles in healthcare is on the increase with silver being used in many devices. However, not all metallic nanoparticles can target and kill all disease-causing bacteria. To overcome this, a combination of several different metallic nanoparticles were used in this study to compare effects of multiple metallic nanoparticles when in combination than when used singly, as single elemental nanoparticles (SENPs), against two common hospital acquired pathogens (Staphylococcus aureus and Pseudomonas. aeruginosa). Flow cytometry LIVE/DEAD assay was used to determine rates of cell death within a bacterial population when exposed to the nanoparticles. Results were analysed using linear models to compare effectiveness of three different metallic nanoparticles, tungsten carbide (WC), silver (Ag) and copper (Cu), in combination and separately. Results show that when the nanoparticles are placed in combination (NPCs), antimicrobial effects significantly increase than when compared with SENPs (P

Published
2019
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