1. High-resolution bioactivity profiling combined with HPLC-HRMS-SPE-NMR: α-Glucosidase inhibitors and acetylated ellagic acid rhamnosides from Myrcia palustris DC. (Myrtaceae).
- Author
-
Wubshet SG, Moresco HH, Tahtah Y, Brighente IMC, and Staerk D
- Subjects
- Chromatography, High Pressure Liquid, Diabetes Mellitus, Type 2 drug therapy, Ellagic Acid chemistry, Flavonoids chemistry, Flavonoids isolation & purification, Flavonoids pharmacology, Glycoside Hydrolase Inhibitors chemistry, Glycosides chemistry, Humans, Hypoglycemic Agents chemistry, Kaempferols chemistry, Kaempferols isolation & purification, Kaempferols pharmacology, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Plant Leaves chemistry, Quercetin analysis, alpha-Glucosidases drug effects, Ellagic Acid isolation & purification, Ellagic Acid pharmacology, Glycoside Hydrolase Inhibitors isolation & purification, Glycoside Hydrolase Inhibitors pharmacology, Glycosides isolation & purification, Glycosides pharmacology, Hypoglycemic Agents isolation & purification, Hypoglycemic Agents pharmacology, Myrtaceae chemistry
- Abstract
Type 2 diabetes (T2D) is an endocrine metabolic disease with a worldwide prevalence of more than 8%, and an expected increase close to 50% in the next 15-20years. T2D is associated with severe and life-threatening complications like retinopathy, neuropathy, nephropathy, and cardiovascular diseases, and therefore improved drug leads or functional foods containing α-glucosidase inhibitors are needed for management of blood glucose. In this study, leaves of Myrcia palustris were investigated by high-resolution α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of casuarinin, myricetin 3-O-β-d-(6″-galloyl)galactopyranoside, kaempferol 3-O-β-d-galactopyranoside, myricetin, and quercetin as α-glucosidase inhibitors. In addition, four acetylated ellagic acid rhamnosides, i.e., 4-O-(2″,4″-O-diacetyl-α-l-rhamnopyranosyl)ellagic acid, 4-O-(2″,3″-O-diacetyl-α-l-rhamnopyranosyl)ellagic acid, 4-O-(3″,4″-O-diacetyl-α-l-rhamnopyranosyl)ellagic acid, and 4-O-(2″,3″,4″-O-triacetyl-α-l-rhamnopyranosyl)ellagic acid were identified., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF