1. Partial characterization of the human beta-myosin heavy-chain gene which is expressed in heart and skeletal muscle.
- Author
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Lichter P, Umeda PK, Levin JE, and Vosberg HP
- Subjects
- Animals, Base Composition, Base Sequence, Cloning, Molecular, DNA analysis, Endonucleases, Exons, Humans, RNA, Messenger analysis, Rabbits, Rats, Single-Strand Specific DNA and RNA Endonucleases, Muscles metabolism, Myocardium metabolism, Myosins genetics
- Abstract
A human myosin heavy-chain gene, cloned in gamma Charon 4A phage (and as a clone designated lambda gMHC-1), was shown to code for a cardiac myosin heavy chain of the beta-type. The 5' end of the 14,200-base-pair genomic DNA clone is located in the head region of the myosin chain. The 3' end was shown to extent to the COOH terminus and includes the 3'-nontranslated sequence of the corresponding mRNA. The identification of lambda gMHC-1 as coding for a cardiac beta-myosin heavy chain was achieved by heteroduplex mapping using genomic cardiac myosin heavy-chain DNA of rabbit as a probe and, furthermore, by DNA sequence analysis of three selected subregions of the clones DNA including the 3'-nontranslated sequence. It was demonstrated by the S1 nuclease protection technique that the beta-myosin heavy-chain gene is transcribed in human heart muscle. In addition, we have found by the same technique that it is also expressed in human skeletal muscle.
- Published
- 1986
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