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Start Over Author "Kenar H" Topic myocardium
3 results on '"Kenar H"'

1. A 3D aligned microfibrous myocardial tissue construct cultured under transient perfusion.

Author

Kenar H, Kose GT, Toner M, Kaplan DL, and Hasirci V

Subjects
Bioreactors, Cell Adhesion drug effects, Cell Differentiation drug effects, Cell Proliferation drug effects, Collagen Type I chemistry, Collagen Type I pharmacology, DNA-Binding Proteins genetics, Decanoates chemistry, Elastic Modulus, Gene Expression genetics, Glycerol analogs & derivatives, Glycerol chemistry, Homeobox Protein Nkx-2.5, Homeodomain Proteins genetics, Humans, MADS Domain Proteins genetics, MEF2 Transcription Factors, Mesenchymal Stem Cells cytology, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Myocytes, Cardiac cytology, Myocytes, Cardiac metabolism, Myogenic Regulatory Factors genetics, Polyesters chemistry, Polymers chemistry, T-Box Domain Proteins genetics, Transcription Factors genetics, Umbilical Cord cytology, Viscoelastic Substances chemistry, Myocardium, Perfusion, Tissue Engineering methods, Tissue Scaffolds chemistry
Abstract

The goal of this study was to design and develop a myocardial patch to use in the repair of myocardial infarctions or to slow down tissue damage and improve long-term heart function. The basic 3D construct design involved two biodegradable macroporous tubes, to allow transport of growth media to the cells within the construct, and cell seeded, aligned fiber mats wrapped around them. The microfibrous mat housed mesenchymal stem cells (MSCs) from human umbilical cord matrix (Wharton's Jelly) aligned in parallel to each other in a similar way to cell organization in native myocardium. Aligned micron-sized fiber mats were obtained by electrospinning a polyester blend (PHBV (5% HV), P(L-D,L)LA (70:30) and poly(glycerol sebacate) (PGS)). The micron-sized electrospun parallel fibers were effective in Wharton's Jelly (WJ) MSCs alignment and the cells were able to retract the mat. The 3D construct was cultured in a microbioreactor by perfusing the growth media transiently through the macroporous tubing for two weeks and examined by fluorescence microscopy for cell distribution and preservation of alignment. The fluorescence images of thin sections of 3D constructs from static and perfused cultures confirmed enhanced cell viability, uniform cell distribution and alignment due to nutrient provision from inside the 3D structure., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Published
2011
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2. Design of a 3D aligned myocardial tissue construct from biodegradable polyesters.

Author

Kenar H, Kose GT, and Hasirci V

Subjects
Actins chemistry, Cell Proliferation, Coronary Vessels pathology, Cytoskeleton metabolism, Equipment Design, Humans, Mesenchymal Stem Cells cytology, Microscopy, Confocal methods, Microscopy, Electron, Scanning methods, Porosity, Umbilical Cord pathology, Biocompatible Materials chemistry, Imaging, Three-Dimensional methods, Myocardium pathology, Polyesters chemistry, Tissue Engineering methods
Abstract

The heart does not regenerate new functional tissue when myocardium dies following coronary artery occlusion, or if it is defective. Ventricular restoration involves excising the infarct and replacing it with a cardiac patch to restore the heart to a more healthy condition. The goal of this study was to design and develop a clinically applicable myocardial patch to replace myocardial infarcts and improve long-term heart function. A basic design composed of 3D microfibrous mats that house mesenchymal stem cells (MSCs) was developed from human umbilical cord matrix (Wharton's Jelly) cells aligned in parallel to each other mimicking the native myocardium. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), poly(L-D,L-lactic acid) (P(L-D,L)LA) and poly(glycerol sebacate) (PGS) were blended and electrospun into aligned fiber mats with fiber diameter ranging between 1.10 and 1.25 microm. The micron-sized parallel fibers of the polymer blend were effective in cell alignment and cells have penetrated deep within the mat through the fiber interstices, occupying the whole structure; 8-9 cell layers were obtained. Biodegradable macroporous tubings were introduced to serve as nutrient delivery route. It was possible to create a thick myocardial patch with structure similar to the native tissue and with a capability to grow.

Published
2010
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3. Design of a 3D aligned myocardial tissue construct from biodegradable polyesters

Author

Vasif Hasirci, Halime Kenar, Gamze Torun Kose, Kenar, H., Kose, G.T., Hasirci, V., and Yeditepe Üniversitesi

Subjects
Scaffold, Materials science, Polyesters, Biomedical Engineering, Biophysics, Bioengineering, Biocompatible Materials, Matrix (biology), Umbilical Cord, Biomaterials, Imaging, Three-Dimensional, Tissue engineering, Humans, Fiber, Cytoskeleton, Cell Proliferation, Microscopy, Confocal, Tissue Engineering, Myocardium, Mesenchymal stem cell, Biomaterial, Mesenchymal Stem Cells, Equipment Design, Coronary Vessels, Actins, Polyester, Biochemistry, Microscopy, Electron, Scanning, Stem cell, Porosity, Biomedical engineering
Abstract

The heart does not regenerate new functional tissue when myocardium dies following coronary artery occlusion, or if it is defective. Ventricular restoration involves excising the infarct and replacing it with a cardiac patch to restore the heart to a more healthy condition. The goal of this study was to design and develop a clinically applicable myocardial patch to replace myocardial infarcts and improve long-term heart function. A basic design composed of 3D microfibrous mats that house mesenchymal stem cells (MSCs) was developed from human umbilical cord matrix (Wharton's Jelly) cells aligned in parallel to each other mimicking the native myocardium. Poly(3-hydroxybutyrate- co-3-hydroxyvalerate) (PHBV), poly(L-D,L-lactic acid) (P(L-D,L)LA) and poly(glycerol sebacate) (PGS) were blended and electrospun into aligned fiber mats with fiber diameter ranging between 1.10 and 1.25 µm. The micron-sized parallel fibers of the polymer blend were effective in cell alignment and cells have penetrated deep within the mat through the fiber interstices, occupying the whole structure; 8-9 cell layers were obtained. Biodegradable macroporous tubings were introduced to serve as nutrient delivery route. It was possible to create a thick myocardial patch with structure similar to the native tissue and with a capability to grow. © 2009 Springer Science+Business Media, LLC. Sixth Framework Programme 105T508, METU-BAP 0108 DPT 2006K 120920-20 Acknowledgments This study was supported by TUBITAK TBAG 105T508, METU-BAP 0108 DPT 2006K 120920-20 and EU FP6 Network of Excellence project EXPERTISSUES.

Published
2009

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