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Your search keyword '"Speciale, L."' showing total 3 results
Start Over Author "Speciale, L." Topic myelin basic protein
3 results on '"Speciale, L."'

1. Programmed cell death of myelin basic protein-specific T lymphocytes is reduced in patients with acute multiple sclerosis.

Author

Saresella M, Marventano I, Speciale L, Ruzzante S, Trabattoni D, Della Bella S, Filippi M, Fasano F, Cavarretta R, Caputo D, Clerici M, and Ferrante P

Subjects
Acute Disease, Adult, Biomarkers metabolism, CD4-Positive T-Lymphocytes drug effects, Case-Control Studies, Female, Humans, Male, Middle Aged, Multiple Sclerosis metabolism, Multiple Sclerosis pathology, Myelin Basic Protein pharmacology, Proto-Oncogene Proteins c-bcl-2 metabolism, Tetradecanoylphorbol Acetate pharmacology, fas Receptor metabolism, Apoptosis, CD4-Positive T-Lymphocytes metabolism, Multiple Sclerosis physiopathology, Myelin Basic Protein metabolism
Abstract

We investigated the apoptosis of myelin basic protein (MBP)-specific T lymphocytes in multiple sclerosis (MS) patients with acute (AMS) or stable (SMS) MS by evaluating the expression of apoptosis markers on peripheral cells. Cells of healthy controls (HC) were evaluated as well. Results showed that mitogen-stimulated apoptosis was comparable among patients and controls, whereas MBP-stimulated CD4+ and CD8+ 7-AAD+ and 7-AAD+ Fas+ cell (apoptotic cells) were significantly reduced in AMS patients. A reduction of the apoptotic rate of myelin-specific CD4+ and CD8+ T lymphocytes could be involved in the immune-mediated destruction of the myelin sheath seen in AMS patients.

Published
2005
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2. A polymorphism in the repetitive (TGGA)n sequence 5' to the human myelin basic protein gene in Italian multiple sclerosis patients.

Author

Guerini FR, Losciale L, Mediati M, Speciale L, Mancuso R, Saresella M, Calvo MG, Caputo D, and Ferrante P

Subjects
Alleles, Amino Acid Sequence, DNA Mutational Analysis, Female, Gene Deletion, Genetic Predisposition to Disease, Humans, Italy, Male, Molecular Sequence Data, Multiple Sclerosis, Chronic Progressive genetics, Multiple Sclerosis, Relapsing-Remitting genetics, Myelin Basic Protein genetics, Polymorphism, Genetic, Repetitive Sequences, Nucleic Acid
Abstract

Human myelin basic protein (hMBP) gene is one of the candidate genes in the complex mosaic of multiple sclerosis (MS) susceptibility. In this study we verified the distribution of the polymorphism of the region 5' flanking the first exon of the hMBP gene, in 97 relapsing remitting, 74 primary progressive Italian MS patients, and in 236 healthy controls, using polymerase chain reaction (PCR) and gel electrophoresis analysis in this region from 1116 - 1540 nt. Three different band patterns were observed: one homozygote with a 354 bp long fragment, one homozygote with 424 bp long fragment and one heterozygote with both bands present. The short fragment was statistically more frequent in RRMS patients than in HC (P<0.05). The long fragment was more present in HC. Similarly the short homozygous pattern (354 bp/354 bp) was significantly higher in the RRMS patients versus the healthy controls (P<0.01). The sequence analysis of the hMBP alleles showed that while the long fragments matched the prototype sequence completely, all the short fragments showed a deletion of 70 bp from nt 1177 to nt 1247, which explains the short 354 bp allele detected by PCR. Moreover two single mismatches in positions 1386 (T-->C) and 1431 (G-->A), were present only in the short hMBP fragment.

Published
2000

3. Single-cell analysis of cytokine production shows different immune profiles in multiple sclerosis patients with active or quiescent disease

Author

Domenico Caputo, Rosella Cavaretta, Marina Saresella, Massimo Filippi, Mario Clerici, Sabrina Fossati, Livianna Speciale, Pasquale Ferrante, Stefania Ruzzante, Daria Trabattoni, Clerici, M, Saresella, M, Trabattoni, D, Speciale, L, Fossati, S, Ruzzante, S, Cavaretta, R, Filippi, Massimo, Caputo, D, and Ferrante, P.

Subjects
Adult, CD4-Positive T-Lymphocytes, Male, medicine.medical_treatment, CD14, Immunology, Lipopolysaccharide Receptors, CD8-Positive T-Lymphocytes, In Vitro Techniques, Peripheral blood mononuclear cell, Enterotoxins, Interferon-gamma, Multiple Sclerosis, Relapsing-Remitting, Immune system, Adjuvants, Immunologic, CD28 Antigens, medicine, Humans, Immunology and Allergy, Longitudinal Studies, Immunity, Cellular, biology, Interleukin-6, Tumor Necrosis Factor-alpha, Multiple sclerosis, Antibodies, Monoclonal, Myelin Basic Protein, Interferon-beta, Middle Aged, medicine.disease, Interleukin-12, Myelin basic protein, Cytokine, Neurology, biology.protein, Cytokines, Interleukin-2, Female, Tumor necrosis factor alpha, Neurology (clinical), Antibody, Interleukin-1
Abstract

Peripheral blood mononuclear cells of multiple sclerosis (MS) patients were stimulated with myelin basic protein (MBP) together with anti-CD28 monoclonal antibody and staphylococcal enterotoxin B to optimize cytokine production by antigen-specific cells. Type 1 (IL-2, IL-12, IFNgamma) and pro-inflammatory (TNFalpha, IL-1beta, IL-6) cytokines were augmented in CD4+, CD8+, and CD14+ cells of acute MS patients and of patients undergoing disease reactivation. These cytokines were reduced in IFNbeta-treated and in stable MS patients; type 2 cytokines (IL-4, IL-10) were increased in these patients. Similar immune profiles are seen in MS patients in whom remission is naturally or pharmacologically (IFNbeta) achieved. Cytokine alterations are particularly evident in CD14+ cells, underlying their critical role in the modulation of the immune response.

Published
2001
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