1. Pathogenic Cav3.2 channel mutation in a child with primary generalized epilepsy.
- Author
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Souza IA, Gandini MA, Zhang FX, Mitchell WG, Matsumoto J, Lerner J, Pierson TM, and Zamponi GW
- Subjects
- Biophysical Phenomena, Child, Female, Humans, Infant, Infant, Newborn, Calcium Channels, T-Type genetics, Epilepsy, Generalized genetics, Mutation genetics
- Abstract
Two paternally-inherited missense variants in CACNA1H were identified and characterized in a 6-year-old child with generalized epilepsy. Febrile and unprovoked seizures were present in this child. Both variants were expressed in cis or isolation using human recombinant Cav3.2 calcium channels in tsA-201 cells. Whole-cell patch-clamp recordings indicated that one variant (c.3844C > T; p.R1282W) caused a significant increase in current density consistent with a pathogenic gain-of-function phenotype; while the other cis-related variant (c.5294C > T; p.A1765V) had a benign profile.
- Published
- 2019
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