1. Muscle phenotype of a rat model of Duchenne muscular dystrophy.
- Author
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Iyer SR, Xu S, Shah SB, and Lovering RM
- Subjects
- Animals, Animals, Genetically Modified, Dystrophin genetics, Gene Knockout Techniques, Hindlimb, Magnetic Resonance Imaging, Male, Muscle Contraction genetics, Muscle Strength genetics, Muscle, Skeletal diagnostic imaging, Muscle, Skeletal pathology, Muscular Dystrophy, Duchenne diagnostic imaging, Muscular Dystrophy, Duchenne genetics, Muscular Dystrophy, Duchenne pathology, Phenotype, Quadriceps Muscle diagnostic imaging, Quadriceps Muscle pathology, Quadriceps Muscle physiopathology, Disease Models, Animal, Muscle Contraction physiology, Muscle Strength physiology, Muscle, Skeletal physiopathology, Muscular Dystrophy, Duchenne physiopathology, Rats
- Abstract
Introduction: Our aim was to assess key muscle imaging and contractility parameters in the Duchenne muscular dystrophy (DMD) rat model (Dmd-KO rat), which have not yet been characterized sufficiently., Methods: We performed in-vivo magnetic resonance imaging (MRI) for thigh and leg muscles, and performed hematoxylin and eosin (H&E) staining and in-vivo muscle contractility testing in specific hindlimb muscles., Results: MRI prior to testing muscle contractility revealed multiple, unevenly distributed focal hyperintensities in the Dmd-KO rat quadriceps and tibialis anterior muscles. H&E staining showed corresponding areas of inflammation and ongoing regeneration. In-vivo contractile testing showed maximal force generated by Dmd-KO muscles was significantly lower, and susceptibility to injury was ~ two-fold greater in the Dmd-KO rats compared to wild-type (WT) rats., Discussion: Together, the MRI findings, histological findings, and the low strength and high susceptibility to injury in muscles support use of the Dmd-KO rat as an animal model of DMD., (© 2020 Wiley Periodicals LLC.)
- Published
- 2020
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