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In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and Nicotiana tabacum. Agroinfiltration induced yellow mosaic symptoms in mungbean and N. tabacum plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers. [ABSTRACT FROM AUTHOR]

The yellow mosaic symptoms on soybean (Glycine max) were observed at various sites in the Bundelkhand region of India and infection of begomovirus was detected through PCR. The begomovirus isolates were identified as Mungbean yellow mosaic India virus (MYMIV). MYMIV infection in soybean results in the production of reactive oxygen species to activate defense mechanisms at different levels to overcome the damage caused in the severely infected crops. The biomarkers of stress in plants such as, hydrogen peroxide (H2O2), catalase (CAT), Superoxide dismutase (SOD), Ascorbate peroxidase (APX) contents studies also suggested elevation in stress-related enzymes and also a significant reduction in sugar, chlorophyll, and protein contents have been observed. Previously alteration in biochemical due biotic and abiotic stresses have been reported in different varieties of legumes, but biochemical alterations in soybean infected by MYMIV are being reported for the first time from the Bundelkhand region of India. [ABSTRACT FROM AUTHOR]