1. Method for coregistration of optical measurements of breast tissue with histopathology: the importance of accounting for tissue deformations
- Author
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Esther Kho, Theo J.M. Ruers, Leon C. ter Beek, Lisanne L. de Boer, Koen Van de Vijver, Henricus J. C. M. Sterenborg, Jasper Nijkamp, CCA - Imaging and biomarkers, Biomedical Engineering and Physics, Technical Medicine, and Nanobiophysics
- Subjects
Computer science ,H&E stain ,01 natural sciences ,Atomic and Molecular Physics ,REPRODUCIBILITY ,Validation ,Medicine and Health Sciences ,Image Processing, Computer-Assisted ,Breast ,validation ,Breast tissue ,Optical Imaging ,Gold standard ,gold standard ,registration algorithm ,diffuse reflectance ,CANCER ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,VARIABILITY ,Registration algorithm ,histopathology ,Diffuse reflectance ,Female ,Tomography ,Algorithms ,MRI ,Paper ,MARGIN ,medicine.medical_specialty ,Optical measurements ,Biomedical Engineering ,Image registration ,Histopathology ,Breast Neoplasms ,Accounting ,010309 optics ,Biomaterials ,0103 physical sciences ,Electronic ,medicine ,Humans ,Optical and Magnetic Materials ,Optical techniques ,General ,Histocytological Preparation Techniques ,business.industry ,Biology and Life Sciences ,Reproducibility of Results ,Optics ,Tissue characterization ,Gold standard (test) ,SPECIMENS ,PATHOLOGY ,business ,optical techniques - Abstract
For the validation of optical diagnostic technologies, experimental results need to be benchmarked against the gold standard. Currently, the gold standard for tissue characterization is assessment of hematoxylin and eosin (H&E)-stained sections by a pathologist. When processing tissue into H&E sections, the shape of the tissue deforms with respect to the initial shape when it was optically measured. We demonstrate the importance of accounting for these tissue deformations when correlating optical measurement with routinely acquired histopathology. We propose a method to register the tissue in the H&E sections to the optical measurements, which corrects for these tissue deformations. We compare the registered H&E sections to H&E sections that were registered with an algorithm that does not account for tissue deformations by evaluating both the shape and the composition of the tissue and using microcomputer tomography data as an independent measure. The proposed method, which did account for tissue deformations, was more accurate than the method that did not account for tissue deformations. These results emphasize the need for a registration method that accounts for tissue deformations, such as the method presented in this study, which can aid in validating optical techniques for clinical use. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
- Published
- 2019