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Your search keyword '"Tastemel M"' showing total 2 results
Start Over Author "Tastemel M" Topic mouse embryonic stem cells
2 results on '"Tastemel M"'

1. Transcription pausing regulates mouse embryonic stem cell differentiation.

Author

Tastemel M, Gogate AA, Malladi VS, Nguyen K, Mitchell C, Banaszynski LA, and Bai X

Subjects
Animals, Chromatin metabolism, Chromosomal Proteins, Non-Histone genetics, Chromosomal Proteins, Non-Histone metabolism, Histones metabolism, Mice, Mouse Embryonic Stem Cells metabolism, Promoter Regions, Genetic, RNA Polymerase II genetics, RNA Polymerase II metabolism, Transcriptional Elongation Factors genetics, Transcriptional Elongation Factors metabolism, Cell Differentiation, Mouse Embryonic Stem Cells cytology, Transcription, Genetic
Abstract

The pluripotency of embryonic stem cells (ESCs) relies on appropriate responsiveness to developmental cues. Promoter-proximal pausing of RNA polymerase II (Pol II) has been suggested to play a role in keeping genes poised for future activation. To identify the role of Pol II pausing in regulating ESC pluripotency, we have generated mouse ESCs carrying a mutation in the pause-inducing factor SPT5. Genomic studies reveal genome-wide reduction of paused Pol II caused by mutant SPT5 and further identify a tight correlation between pausing-mediated transcription effect and local chromatin environment. Functionally, this pausing-deficient SPT5 disrupts ESC differentiation upon removal of self-renewal signals. Thus, our study uncovers an important role of Pol II pausing in regulating ESC differentiation and suggests a model that Pol II pausing coordinates with epigenetic modification to influence transcription during mESC differentiation., (Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2017
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2. CTCF mediates dosage- and sequence-context-dependent transcriptional insulation by forming local chromatin domains

Author

Miao Yu, Bing Ren, Andrea M. Chiariello, Yuanyuan Han, Mattia Conte, Adam Jussila, Yanxiao Zhang, Quan Zhu, Ivan Juric, Xiaowei Zhuang, Hui Huang, Bogdan Bintu, Melodi Tastemel, Ming Hu, Mario Nicodemi, Simona Bianco, Rong Hu, Colin Kern, Huang, H., Zhu, Q., Jussila, A., Han, Y., Bintu, B., Kern, C., Conte, M., Zhang, Y., Bianco, S., Chiariello, A. M., Yu, M., Hu, R., Tastemel, M., Juric, I., Hu, M., Nicodemi, M., Zhuang, X., and Ren, B.

Subjects
CCCTC-Binding Factor, Transcription, Genetic, Context (language use), Plasma protein binding, Biology, Insulator Element, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Transcription (biology), Genetics, Animals, Humans, Binding site, Promoter Regions, Genetic, computational data analyses, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, Binding Sites, Mouse Embryonic Stem Cells, Mouse Embryonic Stem Cell, Chromatin, Cell biology, Enhancer Elements, Genetic, Gene Expression Regulation, CTCF, Insulator Elements, 030217 neurology & neurosurgery, Function (biology), Human, Protein Binding
Abstract

Insulators play a critical role in spatiotemporal gene regulation in animals. The evolutionarily conserved CCCTC-binding factor (CTCF) is required for insulator function in mammals, but not all of its binding sites act as insulators. Here we explore the sequence requirements of CTCF-mediated transcriptional insulation using a sensitive insulator reporter in mouse embryonic stem cells. We find that insulation potency depends on the number of CTCF-binding sites in tandem. Furthermore, CTCF-mediated insulation is dependent on upstream flanking sequences at its binding sites. CTCF-binding sites at topologically associating domain boundaries are more likely to function as insulators than those outside topologically associating domain boundaries, independently of binding strength. We demonstrate that insulators form local chromatin domain boundaries and weaken enhancer-promoter contacts. Taken together, our results provide genetic, molecular and structural evidence connecting chromatin topology to the action of insulators in the mammalian genome.

Published
2021

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