Three hundred seventy-seven specimens, distributed in 13 species of mosquitoes, were collected between June and November 2011 in a forest area in the Parque Natural do Vale do Rio do Peixe, Municipality of Joaçaba, Santa Catarina. The three most abundant species were: Aedes crinifer (62.3%), Psorophora ferox (23.6%) e Trichoprosopon pallidiventer (5.1%). Regarding to the abundance and richness of mosquitoes according to the month, it was found that October had the highest abundance of mosquitoes (33.34%), followed by August (23.34%) and November (20.42 %). Concerning to the richness, October had the highest number of species (11), followed by November (9) and August (6). It was also observed the abundance and richness of the collection schedules, and the time between 11 am and 12 am showed higher abundance and richness. In contrast, the time of lowest abundance and richness was from 8 am to 9 am. Three species of Culicidae were firstly reported to the Santa Catarina State (Mansonia indubitans, Sabethes whitmani and Wyeomyia sabethea). The possible medical and veterinary significance of the findings is discussed. [ABSTRACT FROM AUTHOR]