Artemisia calcicola (Asteraceae, Anthemideae), a new species from karst region in Shibing county, Guizhou province, southwestern China, is described and illustrated. The species can be readily assigned to A. subg. Artemisia in having fertile disk florets and glabrous receptacles. Within this subgenus, A. calcicola is distinguished by having (2- or) 3-pinnatipartite leaves and narrowly ellipsoid involucres 0.9–1.3 mm in diameter. It resembles A. annua to some extent, but differs immediately by the plant duration, stem and leaf indumentum, and involucre shape and size. A detailed description and distribution map of this species are also provided herein.