1. Co-infection with distinct Trypanosoma cruzi strains induces an activated immune response in human monocytes.
- Author
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Magalhães LMD, Passos LSA, Chiari E, Galvão LMC, Koh CC, Rodrigues-Alves ML, Giunchetti RC, Gollob K, and Dutra WO
- Subjects
- Adolescent, Adult, Cells, Cultured, Chagas Disease parasitology, Coinfection, Humans, Interleukin-10 metabolism, Middle Aged, Tumor Necrosis Factor-alpha metabolism, Young Adult, Chagas Disease immunology, Monocytes immunology, Trypanosoma cruzi classification, Trypanosoma cruzi immunology
- Abstract
Aims: The aim of the study was to evaluate the immune response triggered by the first contact of human monocytes with two T cruzi strains from distinct discrete typing units (DTUs) IV and V, and whether co-infection with these strains leads to changes in monocyte immune profiles, which could in turn influence the subsequent infection outcome., Methods and Results: We evaluated the influence of in vitro single- and co-infection with AM64 and 3253 strains on immunological characteristics of human monocytes. Single infection of monocytes with AM64 or 3253 induced opposing anti-inflammatory and inflammatory responses, respectively. Co-infection was observed in over 50% of monocytes after 15 hours of culture, but this percentage dropped ten-fold after 72 hours. Co-infection led to high monocyte activation and an increased percentage of both IL-10 and TNF. The decreased percentage of co-infected cells observed after 72 hours was associated with a decreased frequency of TNF-expressing cells., Conclusion: Our results show that the exacerbated response observed in co-infection with immune-polarizing strains is associated with a decreased frequency of co-infected cells, suggesting that the activated response favours parasite control. These findings may have implications for designing new Chagas disease preventive strategies., (© 2019 John Wiley & Sons Ltd.)
- Published
- 2019
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