1. Composition of the lymphoid cell populations from omental milky spots during the immune response in C57BL/Ka mice
- Author
-
Robert V. Rouse, Bruno Kyewski, Short Paper, and Kazimir Dux
- Subjects
B-Lymphocytes ,Stromal cell ,medicine.drug_class ,T-Lymphocytes ,Immunology ,Cell ,Mice, Inbred Strains ,T lymphocyte ,Biology ,Monoclonal antibody ,Mice ,medicine.anatomical_structure ,Lymphatic system ,Immune system ,Antigen ,medicine ,Animals ,Immunology and Allergy ,Cytotoxic T cell ,Female ,Lymphocytes ,Omentum - Abstract
The lymphoid cell composition of milky spots was analyzed in unprimed mice before and after i.p. immunization with sheep red blood cells. Milky spots contained surface immunoglobulin-positive B lymphocytes, and T cells of the helper and cytotoxic phenotype. After secondary antigen challenge (a) the number of lymphocytes increased up to 40-fold, (b) B and T cells were found to segregate into distinct areas in situ, and (c) lymphocytes were found to associate with I-A-negative stromal cells in vivo. These findings qualify milky spots as a peripheral lymphoid organ exhibiting a remarkable change in number and composition of lymphocytes in response to a local antigen stimulus.
- Published
- 1986