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Biolabelling, Fluorophores, Metal-Chelating Fluorophores, Self-Healing Dyes, Super-Resolution Microscopy Functional groups were introduced that turned a variety of fluorophores into highly stable self-healing dyes or metal-chelating dyes during biolabelling. Inside Back Cover: Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling (Angew. [Extracted from the article]

Keywords: Biolabelling; Fluorophores; Metal-Chelating Fluorophores; Self-Healing Dyes; Super-Resolution Microscopy EN Biolabelling Fluorophores Metal-Chelating Fluorophores Self-Healing Dyes Super-Resolution Microscopy 1 1 1 04/27/22 20220502 NES 220502 B Die Ugi-4-Komponentenreaktion b wurde von Andreas Herrmann, Thorben Cordes et al. in ihrem Forschungsartikel (e202112959) angewandt, um eine Bibliothek von Linker-Molekülen zu synthetisieren (dargestellt als das ESA Space Gateway), um biologische Ziele (die Raumfähre) mit kommerziell erhältlichen Fluoreszenzfarbstoffen (die Orion-Servicemodule) zu verbinden. Innenrücktitelbild: Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling (Angew. Biolabelling, Fluorophores, Metal-Chelating Fluorophores, Self-Healing Dyes, Super-Resolution Microscopy. [Extracted from the article]

Gap junctions are intercellular conduits that are formed in vertebrates by connexin proteins and allow diffusion exchange of intracellular ions and small molecules. At least 20 different connexin genes in the human and mouse genome are cell-type specifically expressed with overlapping expression patterns. A possible explanation for this diversity could be different permeability of biologically important molecules, such as second messenger molecules. We have recently demonstrated that cyclic nucleotide-gated channels can be used to quantify gap junction-mediated diffusion of cyclic AMP. Using this method we have compared the relative permeability of gap junction channels composed of connexin 26, 32, 36, 43, 45, or 47 proteins toward the second messenger cAMP. Here we show that cAMP permeates through the investigated connexin channels with up to 30-fold different efficacy. Our results suggest that intercellular cAMP signaling in different cell types can be affected by the connexin expression pattern. [ABSTRACT FROM AUTHOR]