1. Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro
- Author
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Colleen M. Higgins, Nicolas Bejerman, Ming Li, Anthony P. James, Ralf G. Dietzgen, Michael N. Pearson, Peter A. Revill, and Robert M. Harding
- Subjects
060506 Virology ,Molecular Sequence Data ,Sequence Homology ,General Medicine ,Genome, Viral ,Sequence Analysis, DNA ,CBDV ,Pacific ,060000 BIOLOGICAL SCIENCES ,Open Reading Frames ,Virology ,Gene Order ,plant rhabdovirus ,Cluster Analysis ,RNA, Viral ,Melanesia ,taro ,Rhabdoviridae ,Phylogeny ,Colocasia ,Plant Diseases - Abstract
We report the first genome sequence of a Colocasia bobone disease-associated virus (CBDaV) derived from bobone-affected taro [Colocasia esculenta L. Schott] from Solomon Islands. The negative-strand RNA genome is 12,193 nt long, with six major open reading frames (ORFs) with the arrangement 3′-N-P-P3-M-G-L-5′. Typical of all rhabdoviruses, the 3′ leader and 5′ trailer sequences show complementarity to each other. Phylogenetic analysis indicated that CBDaV is a member of the genus Cytorhabdovirus, supporting previous reports of virus particles within the cytoplasm of bobone-infected taro cells. The availability of the CBDaV genome sequence now makes it possible to assess the role of this virus in bobone, and possibly alomae disease of taro and confirm that this sequence is that of Colocasia bobone disease virus (CBDV).
- Published
- 2015