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An organic-inorganic hybrid functionalized material was successfully synthesized by a surface molecular imprinting technique combined with a nonhydrolytic sol-gel (NHSG) process using acrylamide (AA) as the template, methacrylic acid as the functional monomer, methacryloxypropyltrimethoxysilane as the cross-linker and magnetic chitosan (CTS) microspheres as the support material. The adsorption ability and selectivity of this material toward AA was characterized. Experimental results showed that the imprinted polymer exhibited better recognition and selective performance toward AA than the nonimprinted polymer (NIP). The saturated binding capacity (Qmax) was 104.68 mg/g, and the equilibrium dissociation constant was 263.16 mg/mL. This prepared material also offered fast kinetics for the adsorption and desorption of AA. After shaking for 5 min, an adsorption capacity of 4.71 mg/g was obtained, and it almost reached the adsorption equilibrium within 90 min. [ABSTRACT FROM PUBLISHER]

Objective: To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring. Design: Randomized research experimental study. Setting: Academic basic research laboratory. Animal(s): Mice. Intervention(s): Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring. Main Outcome Measure(s): Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19. Result(s): Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3. Conclusion(s): Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring. [Copyright &y& Elsevier]