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A wastewater biofiltration model is used to assess the potential of modelling plant-sized secondary carbon removal biofilter units. Two distinct datasets collected at the Seine-Centre biofiltration plant (Colombes, France) are used. The model is first calibrated on multiple grab samples taken at different heights inside the filter media. Data from 24 hour composite samples at the unit influent and effluent over a 2 year period are then simulated. Additional data are used to estimate hourly concentration profiles from composite samples in order to correctly use both composite and grab samples during modelling. The calibrated model is in most cases able to correctly predict the general nutrient behaviour for both datasets. The results of statistical scores such as the mean error and the mean absolute error are low for soluble components and remain correct for particles during years 2008-2009. Only one parameter set containing few heavily modified values is used to obtain these results. Modelling plant-sized biofilters appears to be practical and can be useful for easily evaluating plant optimization scenarios. [ABSTRACT FROM AUTHOR]