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Introduction:In this study, we investigated the etiopathogenesis of allergic rhinitis by analyzing the polymorphisms including GPx-1 Pro198Leu, CAT-262 C/T, and MnSOD Ala16Val.Methods:The diagnosis of allergic rhinitis was diagnosed by clinical history, examination, serum total immunoglobulin E levels and skin prick test. Five mL of peripheral blood from patients and individuals constituting the control group was taken into EDTA tubes. DNA isolation from whole blood samples was performed according to the Poncz method.Results:Because of this study; for the Pro198Leu polymorphism of the GPX1; was concluded with 95% confidence that the presence of the Leu allele increased the susceptibility to allergic rhinitis 1.092 times. However, this increase was not found to be statistically significant. For the -262 C/T polymorphism of the CAT gene; was concluded with 95% confidence that the presence of the T-allele increased the susceptibility to allergic rhinitis 27,064 times. This increase was found to be statistically significant. For Ala16Val polymorphism of the Mn-SOD gene; was concluded with 95% confidence that the presence of the Ala allele increased the susceptibility to allergic rhinitis 25,791 times. This increase was found to be statistically significant.Conclusion:A significant relationship was found between allergic rhinitis and the genotypes and the frequencies of alleles in the polymorphisms of the MnSOD and CAT genes. However, no significant relationship was found between allergic rhinitis and the polymorphisms of the GPx-1 gene.

Background: Incomplete combustion of wood releases toxic chemicals. Exposure to these chemicals during charcoal production can modulate redox status of cellular system which may further lead to genomic instability and of antioxidant enzymes. Genetic polymorphism may alter the functioning properties of these enzymes and modulate the response to oxidative stress. Methods: In this study, we analyzed the link between genetic polymorphism and enzyme activity for antioxidant enzymes: MnSOD and GPx-1 in charcoal workers and control population. This study included 77 charcoal workers and 79 demographically matched healthy control subjects. This association was studied using multiple linear regression, adjusted for confounding factors viz. age, consumption habits and exposure duration. Results: SOD activity was lower for TT genotype (3.47 ± 0.66; 5.92 ± 1.08) versus CC genotype (3.47 ± 0.66; 6.67 ± 1.60) in control and charcoal workers respectively. Significant lower GPx-1 activity was found in leu/leu genotype (7.25 ± 0.38; 3.59 ± 0.57) when compared to pro/pro genotype (7.78 ± 0.59; 4.28 ± 0.71) and pro/leu genotype (8.48 ± 0.34; 4.30 ± 0.76) in control population and charcoal workers respectively. A significant difference in the levels of 1-Hydroxypyrene (biomarker of exposure) and SOD and GPx-1 activity (biomarkers of oxidative stress) was evident in exposed group in comparison to the control one. Conclusion: Collectively, our findings suggested that PAH influenced the mode of action of SOD and GPx-1 which were impacted by polymorphism in SOD and GPx-1 gene. Hence, polymorphism of MnSOD and GPx-1 genes were found to play a modulatory role in human susceptibility to oxidative damage induced by wood smoke in charcoal workers. [ABSTRACT FROM AUTHOR]

Introduction: In this study, we investigated the etiopathogenesis of allergic rhinitis by analyzing the polymorphisms including GPx-1 Pro198Leu, CAT-262 C/T, and MnSOD Ala16Val. Methods: The diagnosis of allergic rhinitis was diagnosed by clinical history, examination, serum total immunoglobulin E levels and skin prick test. Five mL of peripheral blood from patients and individuals constituting the control group was taken into EDTA tubes. DNA isolation from whole blood samples was performed according to the Poncz method. Results: Because of this study; for the Pro198Leu polymorphism of the GPX1; was concluded with 95% confidence that the presence of the Leu allele increased the susceptibility to allergic rhinitis 1.092 times. However, this increase was not found to be statistically significant. For the -262 C/T polymorphism of the CAT gene; was concluded with 95% confidence that the presence of the T-allele increased the susceptibility to allergic rhinitis 27,064 times. This increase was found to be statistically significant. For Ala16Val polymorphism of the Mn-SOD gene; was concluded with 95% confidence that the presence of the Ala allele increased the susceptibility to allergic rhinitis 25,791 times. This increase was found to be statistically significant. Conclusion: A significant relationship was found between allergic rhinitis and the genotypes and the frequencies of alleles in the polymorphisms of the MnSOD and CAT genes. However, no significant relationship was found between allergic rhinitis and the polymorphisms of the GPx-1 gene. [ABSTRACT FROM AUTHOR]

Both endogenous factors (genomic variations) and exogenous factors (environmental exposures, lifestyle) impact the balance of reactive oxygen species (ROS). Variants of the ND3 (rs2853826; G10398A) gene of the mitochondrial genome, manganese superoxide dismutase ( MnSOD; rs4880 Val16Ala) and glutathione peroxidase ( GPX-1; rs1050450 Pro198Leu), are purported to have functional effects on regulation of ROS balance. In this study, we examined associations of breast and prostate cancer risks and survival with these variants, and interactions between rs4880-rs1050450, and alcohol consumption-rs2853826. Nested case-control studies were conducted in the Breast and Prostate Cancer Cohort Consortium (BPC3), consisting of nine cohorts. The analyses included over 10726 post-menopausal breast and 7532 prostate cancer cases with matched controls. Logistic regression models were used to evaluate associations with risk, and proportional hazard models were used for survival outcomes. We did not observe significant interactions between polymorphisms in MnSOD and GPX-1, or between mitochondrial polymorphisms and alcohol intake and risk of either breast (p-interaction of 0.34 and 0.98, respectively) or prostate cancer (p-interaction of 0.49 and 0.50, respectively). We observed a weak inverse association between prostate cancer risk and GPX-1 Leu198Leu carriers (OR 0.87, 95% CI 0.79-0.97, p = 0.01). Overall survival among women with breast cancer was inversely associated with G10398 carriers who consumed alcohol (HR 0.66 95% CI 0.49-0.88). Given the high power in our study, it is unlikely that interactions tested have more than moderate effects on breast or prostate cancer risk. Observed associations need both further epidemiological and biological confirmation. [ABSTRACT FROM AUTHOR]