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Main focus is to study the three districts of Punjab as well as the nearby areas where Kajli breed exists. DNA quality and quantity was tested by 1% agarose gel. DNA and amino acid content was also analyzed from the blood samples. Majority of amino acids of different breeds are similar to each other which showed that their origin is similar from Urial. However, Alanine content in sheep located from Sargodha was higher than those located with other areas. . It is suggested that the minute difference in these breeds is due to different kinds of breeding that is done in different areas of Pakistan. Sequence of the cytochrome b gene was visualized by the use of Chromas software. Position of band in similar region showed that all the breed belongs to similar origin. Populations of Mianwali, Khushab and Sargodha regions are clustered into one group. The cluster patterns were concordant with their geographic localities [ABSTRACT FROM AUTHOR]