1. Novel attributes of cell‐free plasma mitochondrial DNA in traumatic injury.
- Author
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Daly, Grant T., Pastukh, Viktor M., Tan, Yong B., Francis, C. Michael, Aggen, C. Zack, Groark, S. Chris, Edwards, Carson, Mulekar, Madhuri S., Hamo, Mohammad, Simmons, Jon D., Kutcher, Matthew E., Hartsell, Emily M., Dinwiddie, Darrell L., Turpin, Zachary M., Bass, Hank W., Roberts, Justin T., Gillespie, Mark N., and Langley, Raymond J.
- Subjects
MITOCHONDRIAL DNA ,CELL-free DNA ,SINGLE-stranded DNA - Abstract
In this cohort of trauma patients, we find that shorter mtDNA fragment length may be more sensitive for predicting acute patient outcomes than mtDNA DAMP abundance. Plasma mitochondrial DNA (mtDNA) fragment abundance has emerged as a biomarker in multiple human disorders, thus pointing to the prospect that mtDNA, like nuclear DNA (nDNA), could be a useful substrate for liquid biopsy.1-4 Structural attributes of plasma mtDNA fragments, may contain prognostic information beyond quantitative-polymerase chain reaction (PCR) measured abundance.5,6 While deep sequencing could be informative, the method is limited by the low concentration of mtDNA relative to nDNA in plasma.7 In this communication, we describe a combined target-bait enrichment, sequencing and analytical protocol to improve quantitation and structural insights into plasma mtDNA fragments. Enrichment efficiency was calculated for mean coverage of the mtDNA genome (reads/base) after whole genome sequencing and after target-bait enrichment in four individual patients. [Extracted from the article]
- Published
- 2022
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