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Your search keyword '"O'Donohue, M."' showing total 3 results
Start Over Author "O'Donohue, M." Topic microscopy, electron
3 results on '"O'Donohue, M."'

1. Visualizing macromolecules with fluoronanogold: from photon microscopy to electron tomography.

Author

Cheutin T, Sauvage C, Tchélidzé P, O'Donohue MF, Kaplan H, Beorchia A, and Ploton D

Subjects
Cells, Cultured, Humans, Imaging, Three-Dimensional, Microscopy, Confocal, Microtomy, Photons, Staining and Labeling methods, Fluorescent Dyes chemistry, Gold chemistry, Immunohistochemistry methods, Ki-67 Antigen analysis, Microscopy, Electron methods, RNA Polymerase I analysis
Published
2007
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2. Electron tomography of amplified nanogold immunolabelling: Improvement of quality based on alignment of projections with sinograms and use of post-reconstruction deconvolution.

Author

Tchelidze P, Sauvage C, Bonnet N, Kilian L, Beorchia A, O'Donohue MF, Ploton D, and Kaplan H

Subjects
Gold chemistry, Green Fluorescent Proteins analysis, Green Fluorescent Proteins genetics, Humans, Image Processing, Computer-Assisted methods, KB Cells, Microscopy, Immunoelectron methods, Nanostructures chemistry, Nuclear Proteins analysis, Recombinant Fusion Proteins genetics, Microscopy, Electron methods, Recombinant Fusion Proteins analysis, Tomography, X-Ray Computed methods
Abstract

Electron tomography of immunolabelled proteins identified with amplified nanogold particles imaged by Scanning and Transmission Electron Microscopy within thick sections is a powerful method to investigate the three-dimensional organization of complex cellular machineries. In order to increase the overall quality of the reconstructed cube, we have developed two methods that improve the tomographic reconstruction process. We first performed a very precise alignment of the projections before reconstruction with a technique using sinograms. After reconstruction, we propose to compute image restoration by calculating the Point Spread Function of the projection/back-projection system and to use it to deblur the reconstructed cubes. Improvement in the quality of the reconstructed cubes is demonstrated on images of nucleolar proteins tagged with EGFP and immunolabelled with nanogold particles.

Published
2006
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3. Pictures in cell biology. Spatial dynamics of rRNAs

Author

Cheutin, T., O'Donohue, M., Ploton, D., Thiry, M., Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), inconnu, Inconnu, Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], Active Transport, Cell Nucleus, Biological Transport, Uridine Triphosphate, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Lipid Metabolism, Lipids, Microscopy, Electron, Imaging, Three-Dimensional, RNA, Ribosomal, Humans, Nucleic Acid Conformation, ComputingMilieux_MISCELLANEOUS, HeLa Cells
Abstract

International audience

Published
2001
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