1. Genomic organization and assignment of VAMP2 to 17p12 by FISH.
- Author
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Zoraqi, G. K., Paradisi, S., Falbo, V., and Taruscio, D.
- Subjects
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GENOMES , *FLUORESCENCE in situ hybridization , *GENE mapping , *MICROSATELLITE repeats , *SOMATIC hybrids , *CLONING - Abstract
We describe the complete sequence, genomic organization, and FISH chromosome mapping of the human VAMP2. We identified a 7-kb clone, pISSHG2b3A, containing the entire structure of VAMP2. Previous studies performed by others identified a 5-kb clone, pVPC5-2, containing the incomplete VAMP2. The pVPC5-2 clone was partially sequenced and mapped to the broad region 17pter→p12 by somatic cell hybridization. Our clone overlaps the pVPC5-2 clone and extends ∼2 kb at the 3′ end. In this study, we mapped this gene more precisely on 17p12 by FISH and we found a new polymorphic microsatellite, (GT)[sub 7] CC(GT)[sub 5] , in exon V. This microsatellite, revealing three alleles with frequencies of 0.778, 0.139, and 0.083, might be useful for future linkage studies. Finally, we localized three previously known markers, stSG12859, TIGR-A002F11, and WIAF-1699 (alias stSG4044), in the 3′ untranslated region of the gene. Copyright © 2000 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2000
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