1. Regulation of TGF-β-mediated endothelial-mesenchymal transition by microRNA-27.
- Author
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Suzuki HI, Katsura A, Mihira H, Horie M, Saito A, and Miyazono K
- Subjects
- Animals, Mice, Epithelial-Mesenchymal Transition drug effects, Epithelial-Mesenchymal Transition genetics, MicroRNAs genetics, Transforming Growth Factor beta pharmacology
- Abstract
Multiple microRNAs (miRNAs) regulate epithelial-mesenchymal transition and endothelial-mesenchymal transition (EndMT). Here we report that microRNA-27b (miR-27b) positively regulates transforming growth factor-β (TGF-β)-induced EndMT of MS-1 mouse pancreatic microvascular endothelial cells. TGF-β induced miR-23b/24-1/27b expression, and inhibition of miR-27 suppressed TGF-β-mediated induction of mesenchymal genes. Genome-wide miRNA target analysis revealed that miR-27 targets Elk1, which acts as a competitive inhibitor of myocardin-related transcription factor-serum response factor signalling and as a myogenic repressor. miR-27b was also found to regulate several semaphorin receptors including Neuropilin 2, Plexin A2 and Plexin D1. These results suggest important roles of miR-27 in TGF-β-driven EndMT., (© The Authors 2017. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.)
- Published
- 2017
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