1. Apple ring rot-responsive putative microRNAs revealed by high-throughput sequencing in Malus × domestica Borkh.
- Author
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Yu XY, Du BB, Gao ZH, Zhang SJ, Tu XT, Chen XY, Zhang Z, and Qu SC
- Subjects
- Base Sequence, Disease Resistance, Gene Library, Gene Silencing, High-Throughput Nucleotide Sequencing, Inverted Repeat Sequences, MicroRNAs metabolism, Molecular Sequence Data, RNA, Plant genetics, Sequence Analysis, RNA, Gene Expression Regulation, Plant, Malus genetics, MicroRNAs genetics, Plant Diseases genetics
- Abstract
MicroRNAs (miRNAs) are small non-coding RNAs, which silence target mRNA via cleavage or translational inhibition to function in regulating gene expression. MiRNAs act as important regulators of plant development and stress response. For understanding the role of miRNAs responsive to apple ring rot stress, we identified disease-responsive miRNAs using high-throughput sequencing in Malus × domestica Borkh.. Four small RNA libraries were constructed from two control strains in M. domestica, crabapple (CKHu) and Fuji Naga-fu No. 6 (CKFu), and two disease stress strains, crabapple (DSHu) and Fuji Naga-fu No. 6 (DSFu). A total of 59 miRNA families were identified and five miRNAs might be responsive to apple ring rot infection and validated via qRT-PCR. Furthermore, we predicted 76 target genes which were regulated by conserved miRNAs potentially. Our study demonstrated that miRNAs was responsive to apple ring rot infection and may have important implications on apple disease resistance.
- Published
- 2014
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