3 results on '"Yue-Yue Ding"'
Search Results
2. MiR-223-3p Alleviates Vascular Endothelial Injury by Targeting IL6ST in Kawasaki Disease
- Author
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Xiang Wang, Yue yue Ding, Ye Chen, Qiu qin Xu, Guang hui Qian, Wei guo Qian, Lei Cao, Wan ping Zhou, Miao Hou, and Hai tao Lv
- Subjects
Kawasaki disease ,MicroRNA-223-3p ,IL6ST ,vascular endothelial damage ,STAT3 ,Pediatrics ,RJ1-570 - Abstract
Background: Kawasaki disease (KD) is a self-limiting illness with acute systematic vascular inflammation. It causes pathological changes in mostly medium and small-sized arteries, especially the arteria coronaria, which adds the risk of developing coronary heart disease in adults.Materials and methods: We detected the miR-223-3p expression in 30 KD patients combined with 12 normal controls using miRNA microarrays and RT-PCR. A KD mouse model was constructed using Candida albicans water insoluble substance (CAWS). We also checked the miR-223-3p's expression using qRT-PCR. The Luciferase reporting system was implemented to validate the correlation between miR-223-3p and Interleukin-6 receptor subunit beta (IL-6ST). TNF-α was used to stimulate human coronary artery endothelial cells (HCAECs), and miR-223-3p activator or inhibitor and KD serum were used to treat HCAECs. A Western blotting automatic quantitative analysis protein imprinting system was used to test the expression of signal transducer and the activator of transcription 3 (STAT3), phosphorylated-signal transducer and the activator of transcription 3 (pSTAT3) and NF-κB p65.Results: Clinical trials found that miR-223-3p expressions were markedly different (more than 2-fold) between the acute KD group and the control group. E-selectin and intercellular cell adhesion molecule-1 (ICAM-1) levels were also significantly higher (about 2-fold) in KD especially with coronary artery lesions. MiR-223-3p could alleviate vascular endothelial damage in KD mice, and IL-6 (Interleukin-6), E-selectin and ICAM-1 were simultaneously negative. The values of IL-6, E-selectin, and ICAM-1 mRNA expressions decreased, while the value of IL-6ST was increased between the agonist treated mice and KD mice. The RT-qPCR consequences displayed that miR-223-3p explored the highest expression on the third day in both the KD mice as well as the agonist group. MiR-223-3p can directly combine with IL-6ST 3' untranslatable regions (UTR) and held back the IL-6's expression. Overexpression of miR-223 down regulated IL6ST expression and decreased the expression of p-STAT3 and NF-κB p65, while the miR-223 inhibitor could reverse the above process.Conclusion: MiR-223-3p is an important regulatory factor of vascular endothelial damage in KD and could possibly become a potential target of KD treatment in the future.
- Published
- 2019
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3. Identification of differentially expressed microRNAs in acute Kawasaki disease
- Author
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Yue yue Ding, Ye Chen, Ming Guo Xu, Lei Cao, Yan Ren, Qiu qin Xu, Ling Sun, and Haitao Lv
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Microarray ,Biology ,Mucocutaneous Lymph Node Syndrome ,Biochemistry ,Pathogenesis ,03 medical and health sciences ,microRNA ,Genetics ,Humans ,Gene Regulatory Networks ,RNA, Messenger ,KEGG ,Molecular Biology ,Gene ,Oncogene ,Kawasaki disease ,target gene ,Microarray analysis techniques ,Gene Expression Profiling ,Computational Biology ,Infant ,Articles ,Molecular biology ,microRNA-223-3p ,microRNAs ,body regions ,030104 developmental biology ,Gene Ontology ,Oncology ,Gene Expression Regulation ,Case-Control Studies ,Child, Preschool ,Gene Ontology Term Enrichment ,embryonic structures ,Acute Disease ,Cancer research ,Molecular Medicine ,Female ,Transcriptome ,microarray ,Signal Transduction - Abstract
The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in an independent cohort (n=8). Target genes of the differentially expressed miRNAs were predicted and analyzed for gene ontology term enrichment and Kyoto Encyclopedia of Genes and Genomes pathways. miRNA microarray analysis revealed that seven miRNAs (miRs) were significantly upregulated (hsa-let-7b-5p, hsa-miR-223-3p, hsa-miR-4485, hsa-miR-4644, hsa-miR-4800-5p, hsa-miR-6510-5p and hsa-miR-765) and three were significantly downregulated (hsa-miR-33b-3p, hsa-miR-4443 and hsa-miR-4515) in acute KD compared with the healthy controls. hsa-miR-223-3p expression levels detected by RT-qPCR were consistent with the microarray results. A total of 62 target genes of hsa-miR-223-3p were predicted. In total, 10 differentially expressed miRNAs were identified in acute KD, of which hsa-miR-223-3p was verified by RT-qPCR.
- Published
- 2017
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