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Your search keyword '"Ariyoshi, Jumpei"' showing total 2 results
Start Over Author "Ariyoshi, Jumpei" Topic microrna
2 results on '"Ariyoshi, Jumpei"'

1. RISCのmicroRNAの保持機構に着目した遺伝子制御分子の開発

Author

Ariyoshi, Jumpei

Subjects
microRNA, RNA-induced silencing complex, RISC, Anti-miRNA oligonucleotide, Non-coding RNA, AMO
Abstract

MicroRNAs (miRNAs) are short non-coding RNAs, which play important roles in many biological functions in multicellular organisms. In recent reports, aberrant expressions of miRNAs in several pathological processes, including those in cancers, have been described. Therefore, miRNAs have been considered as promising therapeutic targets. The miRNA duplex associates with the Argonaute protein (Ago) to form ribonucleoprotein complexes called RNA-induced silencing complex (RISC). MiRNA is a core component of RISC and is an essential to RISC for recognizing target mRNA. Thereby, it is expected that development of the method to promote the release of miRNA from RISC would be an effective approach for inhibition of RISC activity. This study presents development of the novel anti-miRNA oligonucleotides (AMOs) to promote the release of miRNA from RISC.

Published
2017

2. Effective Anti-miRNA Oligonucleotides Show High Releasing Rate of MicroRNA from RNA-Induced Silencing Complex.

Author

Ariyoshi, Jumpei, Matsuyama, Yohei, Kobori, Akio, Murakami, Akira, Sugiyama, Hiroshi, and Yamayoshi, Asako

Subjects
*MICRORNA, *GENETIC regulation, *GENE silencing, *ANTINEOPLASTIC agents, *THERAPEUTIC use of oligonucleotides, *THERAPEUTICS
Abstract

MicroRNAs (miRNAs) regulate gene expression by forming RNA-induced silencing complexes (RISCs) and have been considered as promising therapeutic targets. MiRNA is an essential component of RISC for the modulation of gene expression. Therefore, the release of miRNA from RISC is considered as an effective method for the inhibition of miRNA functions. In our previous study, we reported that anti-miRNA oligonucleotides (AMOs), which are composed of the 2′- O-methyl (2′-OMe) RNA, could induce the release of miRNA from RISC. However, the mechanisms underlying the miRNA-releasing effects of chemically modified AMOs, which are conventionally used as anti-cancer drugs, are still unclear. In this study, we investigated the relationship between the miRNA releasing rate from RISC and the inhibitory effect on RISC activity (IC50) using conventional chemically modified AMOs. We demonstrated that the miRNA-releasing effects of AMOs are directly proportional to the IC50 values, and AMOs, which have an ability to promote the release of miRNA from RISC, can effectively inhibit RISC activity in living cells. [ABSTRACT FROM AUTHOR]

Published
2017
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