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The aim of this study was an attempt to determine the influence of PGRs - auxins, cytokinins and gibberellic acid - on the flowering and morphogenesis of ‘Flash Red’ Petunia × atkinsiana D. Don in vitro. The best results at the propagation stage were obtained on an MS medium supplemented with 0.5 mg dm-3 GA3. The petunia plants propagated under these conditions developed high main shoots and a large number of leaves and adventitious shoots. The plants with the best-developed root system were regenerated on an MS medium supplemented with 0.5 and 1.0 mg dm-3 IAA. Flowering initiation should be performed using an MS medium supplemented with 0.5 mg dm-3 KIN.

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Copyright of Folia Horticulturae is the property of Sciendo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to determine the effect of gold and silver nanoparticles on the activity of antioxidant enzymes (ascorbate peroxidase (APX), superoxide dismutase (SOD), guaiacol peroxidase (POX), and catalase (CAT)), the free radical scavenging capacity, and the total polyphenol capacity of lavender (Lavandula angustifolia Mill.) cultivar "Munstead" propagated in vitro. In the experiment, fragments of lavender plants were cultivated in vitro on medium with the addition of 1, 2, 5, 10, 20, and 50 mg∙dm−3 of AgNPs or AuNPs (particle sizes 24.2 ± 2.4 and 27.5 ± 4.8 nm, respectively). It was found that the nanoparticles increase the activity of the antioxidant enzymes APX and SOD; however, the reaction depends on the NP concentration. The highest APX activity is found in plants propagated on media with 2 and 5 mg∙dm−3 of AgNPs. AuNPs significantly increase the APX activity when added to media with a concentration of 10 mg∙dm−3. The highest SOD activity is recorded at 2 and 5 mg∙dm−3 AgNP and AuNP concentrations. The addition of higher concentrations of nanoparticles to culture media results in a decrease in the APX and SOD activity. The addition of AuNPs to culture media at concentrations from 2 to 50 mg∙dm−3 increases the POX activity in comparison to its activity when AgNPs are added to the culture media. No significant influence of NPs on the increase in CAT activity was demonstrated. AgNPs and AuNPs increased the free radical scavenging capacity (ABTS•+). The addition of NPs at concentrations of 2 and 5 mg∙dm−3 increased the production of polyphenols; however, in lower concentrations it decreased their content in lavender tissues. [ABSTRACT FROM AUTHOR]

The sea sandwort—Honckenya peploides (L.) Ehrh. is—a rare halophilous plant growing on dunes and is an endangered species on the Polish coast. It contributes to the stabilization of volatile sandy substrate, facilitating the colonization of other species. The present study determined the reaction of two types of explant: apical shoot fragments and fragments from a lower portion of the shoot. Apical shoot fragments were used to propagate and root sea sandwort plants due to the positive impact on the development of shoots and roots. Regardless of the plant growth regulators applied in the medium, the lateral meristems on the explants from the lower parts of the shoot stopped growing, and then yellowed and died out. Apical fragments of shoots developed higher and more numerous shoots and longer and more numerous roots than explants, which were fragments collected from lower parts of shoots. The findings indicated that propagation should be conducted on Murashige and Skoog medium with the addition of 1 mg∙dm−3 kinetin, whereas shoots with their apical fragments should be rooted with the addition of 1.5 mg∙dm−3 1-naphthaleneacetic acid. The results also showed that the addition of NaCl at concentrations of 25 and 50 mM did not restrict their growth, thereby indicating the tolerance of the plant to soil salinity. However, an increase in the concentration of NaCl in the medium to 75 mM restricted the development of plants, and the shoots were lower and roots were shorter and less numerous. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine how the addition of gold and silver nanoparticles to culture media affects the composition of essential oils extracted from Lavandula angustifolia propagated on MS media with the addition of 10 and 50 mg·dm−3 of gold (24.2 ± 2.4 nm) and silver (27.5 ± 4.8 nm) nanocolloids. The oil extracted from the lavender tissues propagated on the medium with 10 mg·dm−3 AgNPs (silver nanoparticles) differed the most with respect to the control; oil-10 compounds were not found at all, and 13 others were detected which were not present in the control oil. The addition of AuNPs (gold nanoparticles) and AgNPs to the media resulted in a decrease of lower molecular weight compounds (e.g., α- and β-pinene, camphene, δ-3-carene, p-cymene, 1,8-cineole, trans-pinocarveol, camphoriborneol), which were replaced by those of a higher molecular weight (τ- and α-cadinol 9-cedranone, cadalene, α-bisabolol, cis-14-nor-muurol-5-en-4-one, (E,E)-farnesol). [ABSTRACT FROM AUTHOR]