1. Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.
- Author
-
Castillo-Fernandez O, Salieb-Beugelaar GB, van Nieuwkasteele JW, Bomer JG, Arundell M, Samitier J, van den Berg A, and Eijkel JC
- Subjects
- Adsorption, Bacteriophage lambda chemistry, Bacteriophage lambda genetics, DNA, Viral analysis, Electrophoresis methods, Microfluidic Analytical Techniques methods, Polymers, Silicon Dioxide chemistry, Statistics, Nonparametric, DNA, Viral chemistry, Electrophoresis instrumentation, Microfluidic Analytical Techniques instrumentation
- Abstract
The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation., (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2011
- Full Text
- View/download PDF