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A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses. [ABSTRACT FROM AUTHOR]

Abstract: A pathogen detection platform that integrates electrokinetically accelerated sampling with electromechanical signal transduction is presented. Detection of pathogens without the need for cultivation is achieved by employing a prototype device that combines microelectrodes embedded directly onto the surface of a cantilever beam. Escherichia coli bacteria captured on the surface of the cantilever with the aid of AC electrokinetic effects (dielectrophoresis) within 30min of sampling time were found to cause substantial resonant frequency shifts compared to unassisted capture. The effect of the cantilever''s resonant mode was also examined in order to identify conditions that can improve detection sensitivity. [Copyright &y& Elsevier]

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