1. Biological degradation of algae and microcystins by microbial enrichment on artificial media
- Author
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Ji, Rong P., Lu, Xi W., Li, Xian N., and Pu, Yue P.
- Subjects
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BIODEGRADATION , *ALGAE , *MICROCYSTINS , *CHLOROPHYLL , *HIGH performance liquid chromatography , *CHROMATOGRAPHIC analysis , *POLYMERASE chain reaction , *ELECTROPHORESIS , *CULTURE media (Biology) - Abstract
Abstract: The removal efficiency of algae and microcystins (MCs) of a pilot setup based on biological degradation of enrichment microbes by artificial media was studied. The results showed that when chlorophyll-a in Lake Taihu was 3.76–266.1μgL−1, 62.8% of chlorophyll-a could be removed within a hydraulic retention time of 6–7 days. High-performance liquid chromatography (HPLC) analysis for the detection of MCs was applied. When total microcystins RR and LR (TMC-RR and TMC-LR) and extracellular microcystins RR and LR (EMC-RR and EMC-LR) were 0.23–8.93, 0.14–4.73, 0.12–1.15, 0.02–0.17μgL−1, respectively, and in source water, the average removal efficiencies of TMC-RR, TMC-LR, EMC-RR, EMC-LR were 67.0%, 40.5%, 40.0% and 66.0%, respectively. The polymerase chain reaction (PCR) electrophoresis chart revealed that there was a large amount of algae-lysing bacteria such as Pseudomonas spp. and Bacillus spp. on the artificial medium. The protozoa number in the assembled medium was higher than in lake water. Enrichment microbes on the artificial medium could effectively degrade algae and microcystins in Lake Taihu. [Copyright &y& Elsevier]
- Published
- 2009
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