1. Nitrifying biomass characterization and monitoring during bioaugmentation in a membrane bioreactor.
- Author
-
D'Anteo S, Mannucci A, Meliani M, Verni F, Petroni G, Munz G, Lubello C, Mori G, and Vannini C
- Subjects
- Bacteria metabolism, Biomass, DNA, Bacterial genetics, DNA, Bacterial metabolism, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S genetics, RNA, Ribosomal, 16S metabolism, Sequence Analysis, DNA, Bacteria genetics, Bioreactors microbiology, Microbiota, Nitrification, Nitrogen metabolism
- Abstract
A membrane bioreactor (MBR), fed with domestic wastewater, was bioaugmented with nitrifying biomass selected in a side-stream MBR fed with a synthetic high nitrogen-loaded influent. Microbial communities evolution was monitored and comparatively analysed through an extensive bio-molecular investigation (16S rRNA gene library construction and terminal-restriction fragment length polymorphism techniques) followed by statistical analyses. As expected, a highly specialized nitrifying biomass was selected in the side-stream reactor fed with high-strength ammonia synthetic wastewater. The bioaugmentation process caused an increase of nitrifying bacteria of the genera Nitrosomonas (up to more than 30%) and Nitrobacter in the inoculated MBR reactor. The overall structure of the microbial community changed in the mainstream MBR as a result of bioaugmentation. The effect of bioaugmentation in the shift of the microbial community was also verified through statistical analysis.
- Published
- 2015
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