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Start Over Descriptor "thermoextract" Topic microbiology
3 results on '"thermoextract"'

1. Immunophenotyping of blood cells of experimental animals immunized with Brucella abortus thermoextracts

Author

S. V. Balakhonov, V. I. Dubrovina, V. V. Voitkova, K. M. Korytov, N. L. Barannikova, V. B. Nikolaev, and T. T. Shkaruba

Subjects
brucella abortus, thermoextract, experimental animal, blood, flow cytometry, Microbiology, QR1-502
Abstract

Aim. To study the subpopulational structure of blood cells of the experimental animals immunized with thermoextracts (TE) of Brucella abortus in L- and S-form. Materials and methods. Total 100 certified («Vector», Novosibirsk) outbred mice were immunized with B. abortus I-206 TE in L- and S-form in 20 μg protein dose. After 1, 3, 7, 14 and 21 days of observation the phenotypes (CD45, CD3, CD4, CD8, CD19, CD69) of blood cells were detected. Results. General regularities were revealed after injection of the experimental preparations. So, B. abortus TE in L- and S-form caused the immune response that increased granulocyte number and expression of early activation marker CD69 by T- and B-lymphocytes of blood in early period of observation (1-3 days), decrease in general B-lymphocyte content in late periods of observation (7-21 days). Thus, mice received B. abortus ТE in L-form demonstrated authentically higher CD69 expression of blood lymphocyte subpopulations than mice received B. abortus ТE in S-form. Distinctions in formation of humoral immune response were revealed that probably was connected with alteration of Brucella chemical composition in the course of L-transformation. Conclusion. The investigation established that B. abortus TE in L- or S-form caused immunological reorganization in the experimental animal organisms. On the basis of the fin

Published
2019
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2. PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA

Author

L. M. Мikhailov, N. L. Barannikova, L. E. Tokareva, N. M. Andreevskaya, V. A. Мikhailova, V. I. Kuznetsov, K. Yu. Yastremskaya, and S. V. Balakhonov

Subjects
Microbiology (medical), biology, Epidemiology, monospecific serum, thermoextract, Immunology, Heterologous, Brucella, Infectious and parasitic diseases, RC109-216, biology.organism_classification, bacterial infections and mycoses, Microbiology, Virology, Titer, Infectious Diseases, brucella, High activity, s-form, Brucella melitensis, Human brucellosis
Abstract

Objective of the study is to prepare rabbit monospecific anti-abortus serum to a Brucella S-form thermoextract, as well as to evaluate its activity and specificity. Materials and methods. Utilized was polyvalent hyperimmune serum obtained to Brucella thermoextract. It was adsorbed by a strain of heterologous species Brucella melitensis 16 N. Reference, vaccinal and field Brucella strains were used to estimate the activity and specificity of the serum produced. Results and conclusions. This serum possesses high activity (1:160, 1:320 titer) and specificity, and the results completely coincide with the data specified in the table of differential properties presented in МR 3.1.7.1189-03 “Prophylaxis and laboratory diagnostics of human brucellosis». The outlined method for manufacturing monospecific Brucella anti-abortus serum permits for a considerable decrease in biological hazard for personnel involved in the technological operations.

Published
2017

3. Sensitizing Properties of Antigen Preparations Obtained from Brucella in S- and L-Forms

Author

L. M. Mikhailov, L. E. Tokareva, N. L. Barannikova, and S. V. Balakhonov

Subjects
Microbiology (medical), Epidemiology, thermoextract, Immunology, Infectious and parasitic diseases, RC109-216, Brucella, s- and l-forms, Microbiology, бруцеллы, Antigen, s- и l-форма, термоэкстракт, leukocyte sensibilization, biology, Chemistry, bacterial infections and mycoses, biology.organism_classification, Hypersensitivity reaction, Transformation (genetics), leukocytolysis index, Infectious Diseases, brucella, сенсибилизация лейкоцитов, коэффициент лейкоцитолиза
Abstract

It is known that S-form Brucella causes allergic transformation in a macroorganism, which is manifested as delayed-type hypersensitivity reaction. This capacity in atypical variants (L-forms) is under-investigated. It is demonstrated that corpuscular antigen in B. abortus 19BA vaccine and thermoextract prepared from B. abortus I-206 in S-form when threefold administered cause marked sensibilization; while corpuscular antigen and thermoextract produced from Brucella in L-form, and thermoextract from Brucella in S-form in case of one time administration do not have such an effect on leucocytes of white mice.

Published
2014

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