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[Display omitted] • Optimal conditions for sulfide treatment of sludge to establish PN were determined. • High NAR and NRR were achieved in long-term treating wastewater. • amoA and soxB abundance increased and positively correlated in stable phase of PN. • Nitromonas and Sulfuritalea enriched with Nitrospira reduction in long-term reactor. This study proposes an innovative strategy for achieving PN in synthetic domestic wastewater by side-stream sludge treatment using sulfide as the sole control factor. By conducting controllable batch experiments and response surface analysis, optimal sulfide treatment conditions were firstly determined as 90 mg/L of sulfide, 7.5 of pH, 100 rpm of rotation and 12 h of treatment time. After treatment, half of ammonia oxidizing bacteria (AOB) activity remained, but nitrite oxidizing bacteria (NOB) activity was barely detected. Nitrite accumulation rate of long-term running PN steadily reached 83.9 % with 99.1 % of ammonia removal efficiency. Sulfide treatment increased community diversity and facilitated stability of microbiota functioning with PN phenotype, which might be sustained by the positive correlation between ammonia oxidation gene (amoA) and sulfur oxidation gene (soxB). Correspondingly, the network analysis identified the keystone microbial taxa of persistent PN microbiota as Nitrosomonas , Thauera , Truepera , Defluviimonas and Sulitalea in the later stage of long-term reactor. [ABSTRACT FROM AUTHOR]

Partial nitritation is increasingly regarded as a promising biological nitrogen removal process owing to lower energy consumption and better nitrogen removal performance compared to the traditional nitrification process, especially for the treatment of low carbon wastewater. Regulating microbial community structure and function in sewage treatment systems, which are mainly determined by quorum sensing (QS), by free nitrous acid (FNA) to establish a partial nitritation process is an efficient and stable method. Plenty of research papers reported that QS systems ubiquitously existed in ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), and various novel nitrogen removal processes based on partial nitritation were successfully established using FNA. Although the probability that partial nitritation process might be achieved by the regulation of FNA on microbial community structure and function through the QS system was widely recognized and discussed, the potential role of QS in partial nitritation achievement by FNA and the regulation mechanism of FNA on QS system have not been reviewed. This article systematically reviewed the potential role of QS in the establishment of partial nitritation using FNA to regulate activated sludge flora based on the summary and analysis of the published literature for the first time, and future research directions were also proposed. [ABSTRACT FROM AUTHOR]

Summary: Mud volcanoes (MVs) emit globally significant quantities of methane into the atmosphere, however, methane cycling in such environments is not yet fully understood, as the roles of microbes and their associated biogeochemical processes have been largely overlooked. Here, we used data from high‐throughput sequencing of microbial 16S rRNA gene amplicons from six MVs in the Junggar Basin in northwest China to quantify patterns of diversity and characterize the community structure of archaea and bacteria. We found anaerobic methanotrophs and diverse sulfate‐ and iron‐reducing microbes in all of the samples, and the diversity of both archaeal and bacterial communities was strongly linked to the concentrations of sulfate, iron and nitrate, which could act as electron acceptors in anaerobic oxidation of methane (AOM). The impacts of sulfate/iron/nitrate on AOM in the MVs were verified by microcosm experiments. Further, two representative MVs were selected to explore the microbial interactions based on phylogenetic molecular ecological networks. The sites showed distinct network structures, key species and microbial interactions, with more complex and numerous linkages between methane‐cycling microbes and their partners being observed in the iron/sulfate‐rich MV. These findings suggest that electron acceptors are important factors driving the structure of microbial communities in these methane‐rich environments. [ABSTRACT FROM AUTHOR]

The properties of rocks can be greatly affected by seepage hydrocarbons in petroleum-related mud volcanoes. Among them, the color of sedimentary rocks can reflect the changes of sedimentary environment and weathering history. However, little is known about the microbial communities and their biogeochemical significance in these environments. In this study, contrasting rock samples were collected from the Aiqigou mud volcano on the southern margin of the Junggar Basin in Northwest China as guided by rock colors indicative of redox conditions. The physicochemical properties and mineral composition are similar under the same redox conditions. For example, the content of chlorite, muscovite, quartz, and total carbon were higher, and the total iron was lower under reduced conditions compared with oxidized environments. High-throughput sequencing of 16S rRNA gene amplicons revealed that different functional microorganisms may exist under different redox conditions; microbes in oxidized conditions have higher diversity. Statistical analysis and incubation experiments indicated that the microbial community structure is closely related to the content of iron which may be an important factor for color stratification of continental sedimentary rocks in the Aiqigou mud volcano. The interactions between organics and iron-bearing minerals mediated by microorganisms have also been hypothesized. [ABSTRACT FROM AUTHOR]

Understanding the composition of microbials on the grape carposphere may provide direct guidance for the wine brewing. In this work, 16S rRNA and ITS (Internal Transcribed Spacer) fungal amplicon sequencing were performed to investigate the differences of epiphytic microbial communities inhabiting different varieties of wine grape berries. The results showed that the dominated phylum of different wine grape carpospheres were Proteobacteria, Actinomycetes, Firmicutes, Gemmatimonadete, and Bacteroidetes. The dominant bacterial genera of different wine grape varieties were Pseudomonas, Arthrobacter, Bacillus, Pantoea, Planomicrobium, Massilia, Curtobacterium, Corynebacterium, Cellulomonas, Sphingomonas, and Microvirga. The fungal communities of the grapes were dominated by Ascomycota for all nine wine varieties. The dominant fungal genera on grape carposphere were Alternaria, Cladosporium, unclassified Capnodiales, Phoma, Rhodotorula, Cryptococcus, Aureobasidium, and Epicoccum. Community structure exerts a significant impact on bacterial Bray-Curtis dissimilarity on six red grapes and also a significant bacterial impact on three white grapes. Community structure exerts a significant impact on fungal Bray-Curtis dissimilarity on six red grapes but weak or no fungal impact on three white grapes. The results revealed that grape variety plays a significant role in shaping bacterial and fungal community, varieties can be distinguished based on the abundance of several key bacterial and fungal taxa. [ABSTRACT FROM AUTHOR]

With the rapid expansion of livestock production, the amount of livestock wastewater accumulated rapidly. Lack of biodegradable organic matter makes denitrification of livestock wastewater after anaerobic digestion more difficult. In this study, Myriophyllum aquaticum constructed wetlands (CWs) with efficient nitrogen removal performance were established under different carbon/nitrogen (C/N) ratios. Analysis of community composition reveals the change of M. aquaticum CWs in microbial community structure with C/N ratios. The proportion of Proteobacteria which is one of the dominant phyla among denitrifier communities increased significantly under low C/N ratio conditions. Besides, to obtain cultivable denitrifier that could be added into CWs in situ, 33 strains belonging to phylum Proteobacteria were isolated from efficient M. aquaticum CWs, while the best-performing denitrification strain M3-1 was identified as Bacillus velezensis JT3-1 (GenBank No. CP032506.1). Redundancy analysis and quadratic models showed that C/N ratio had significant effects on disposal of nitrate (NO 3 −-N) and the strains isolated could perform well in denitrification when C/N ratio is relatively low. In addition, they have relatively wide ranges of carbon sources, temperature and a high NO 3 − removal rate of 9.12 mg/(L·hr) at elevated concentrations of 800 mg/L nitrate. Thus, strains isolated from M. aquaticum CWs with low C/N ratio have a practical application value in the treatment of nitrate-containing wastewater. These denitrifying bacteria could be added to CWs to enhance nitrogen removal efficiency of CWs for livestock wastewater with low C/N ratio in the future. [Display omitted] [ABSTRACT FROM AUTHOR]

• CW under 105 mg/L NH 4 +-N concs achieved optimal N removal efficiency. • Higher abundance of NarG and NosZ genes were found in high NH 4 +-N concs. CWs. • Denitrifiers including Rhodococcus.sp were enriched under high NH 4 +-N concs. CWs. • Increasing NH 4 +-N concs. led to enhanced interactions among microbial community. Constructed wetlands (CWs) have received increasing attentions for their N removal performances, especially regarding NH 4 +-N. Different influent NH 4 +-N concentration may influence N removal efficiency in practice, while the effects of different NH 4 +-N concentrations on microorganisms removing N in CWs are poorly understood. In this study, surface flow CWs planted with Myriophyllum (M). aquaticum were established to investigate the influences of different NH 4 +-N concentrations on the composition, structure, and interactions of microbial community. Our findings suggested 105 mg/L NH 4 +-N CWs achieved highest N removal rate, removing 89.30 % NH 4 +-N and 92.34 % TN from the influent. The results of real-time quantitative polymerase chain reactions (qPCR) indicated abundances of nitrifying genes (nxrA) and denitrifying genes (narG , nirS , nirK , and nosZ) were increased by increasing NH 4 +-N concentrations, and the strongest effects were observed in narG (8-fold) and nosZ genes (11-fold). Different NH 4 +-N concentrations was proved to alter composition and structure of microbial communities via high-throughput sequencing, e.g. denitrifiers including Brevendomonas.sp , Dokdonella.sp and Rhodococcus.sp were enriched obviously with increasing NH 4 +-N concentrations. In addition, network showed interactions among microbial populations and positive interactions were dramatically shifted and enhanced by increasing NH 4 +-N concentrations. [ABSTRACT FROM AUTHOR]

As a major reservoir of antibiotics, animal manure contributes a lot to the augmented environmental pressure of antibiotic resistance genes (ARGs). This might be the first study to explore the effects of different ventilation types on the control of ARGs and to identify the relationships between archaeal communities and ARGs during the composting of dairy manure. Several ARGs were quantified via Real-time qPCR and microbial communities including bacteria and archaea were analyzed by High-throughput sequencing during vacuum-type composting (VTC) and positive-pressure composting (PPC). The total detected ARGs and class I integrase gene (intI1) under VTC were significantly lower than that under PPC during each stage of the composting (p < 0.001). The relative abundance of potential human pathogenic bacteria (HPB) which were identified based on sequencing information and correlation analysis decreased by 74.6% and 91.4% at the end of PPC and VTC, respectively. The composition of archaeal communities indicated that methane-producing archaea including Methanobrevibacter , Methanocorpusculum and Methanosphaera were dominant throughout the composting. Redundancy analysis suggested that Methanobrevibacter and Methanocorpusculum were positively correlated with all of the detected ARGs. Network analysis determined that the possible hosts of ARGs were different under VTC and PPC, and provided new sights about potential links between archaea and ARGs. Our results showed better performance of VTC in reducing ARGs and potential HPB and demonstrated that some archaea could also be influential hosts of ARGs, and caution the risks of archaea carrying ARGs. Unlabelled Image [ABSTRACT FROM AUTHOR]

The squeezed liquid from fruit and vegetable waste (LW) presents a unique wastewater challenge, marked by recalcitrance in treatment and amplified design risks with the application of conventional processes. Following coagulation of the squeezed liquid, the majority of particulate matter precipitates. The resulting precipitated floc (LWF) is reclaimed and subsequently utilized for the synthesis of biochar. The present study primarily explores the viability of repurposing LWF as biochar to enhance soil quality and mitigate N 2 O emissions. Findings indicate that the introduction of a 2% proportion of LWFB led to a remarkable 99.5% reduction in total N 2 O emissions in contrast to LWF. Concurrently, LWFB substantially enhanced nutrients content by elevating soil organic carbon (SOC) and nitrogen levels. Utilizing high-throughput sequencing in conjunction with qPCR, the investigation unveiled that the porous structure and substantial specific surface area of LWFB potentially fostered microbial adhesion and heightened diversity within the soil microbial community. Furthermore, LWFB notably diminished the relative abundance of AOB (Nitrosospira , Nitrosomonas), and NOB (Candidatus_Nitrotoga), thereby curbing the conversion of NH 4 + into NO 3 −. The pronounced elevation in nosZ abundance implies that LWFB holds the potential to mitigate N 2 O emissions through a conversion to N 2. [Display omitted] • 2% (w/w) LWFB addition reduced 99.5% N 2 O total emissions from soil. • LWFB increased SOC, NH 4 + contents in soil, and restrained NH 4 + converting into NO 3 −. • Large specific surface area and porous structure of LWFB promoted microbial diversity. • LWFB significantly reduced AOB and amoA genes, compared with LWF. • nosZ gene abundance was significantly increased by LWFB. [ABSTRACT FROM AUTHOR]

In this review, the applications of Myriophyllum -based integrative biotechnology to remove common non-point source (NPS) pollutants, such as nitrogen, phosphorus, heavy metals, and organic pollutants (e.g., pesticides and antibiotics) are summarized. The removal of these pollutants via various mechanisms, including uptake by plant and microbial communities in macrophyte-based treatment systems are discussed. This review highlights the potential use of Myriophyllum biomass to produce animal feed, fertilizer, and other valuable by-products, which can yield cost-effective returns and attract more attention to the regulation and recycling of NPS pollutants. In addition, it demonstrates that utilization of Myriophyllum species is a promising and reliable strategy for wastewater treatment. The future development of sustainable Myriophyllum -based treatment systems is discussed from various perspectives. [ABSTRACT FROM AUTHOR]

Anaerobic ammonium oxidation (Anammox) is expected to play a promising role in treating low C/N ratio wastewater. In this study, nitrogen removal performance and the effects of extracellular polymeric substances (EPS) on anammox activity were investigated in two different reactors, followed by analysis of 16S rRNA high-throughput sequencing. A sequencing batch reactor (SBR) and an up-flow anammox sludge blanket reactor (UASB) operated for 133 days, with total nitrogen removal efficiencies of 82.09 ± 4.76% and 87.91 ± 2.59% in the phase 3, respectively. The EPS concentration in UASB was about 1.6 times higher than that in SBR. Also, in the batch tests, especially the anammox-EPS of UASB (A U -EPS) additions contributed to better nitrogen removal capability for anammox bacteria (AnAOB). The relative abundance of AnAOB in UASB was observed to reach 19.68% in the end, accounting for a relatively high degree in the whole community. In SBR, the effluent NH 4 +-N concentration increased when increasing the substrate concentration, followed by the decrease of the relative abundance of AnAOB. Thus, the different feeding strategies would lead to different granulation styles, further influencing the activity and stability of anammox system. This research aims to get a better understanding of the different mechanisms associated with AnAOB enrichment strategies. [Display omitted] • Addition of Anammox-EPS effectively enhanced the anammox activity. • Adding the Anammox-EPS of UASB led to a higher SAA. • Compared to SBR, there was a higher biomass retention ability in UASB. [ABSTRACT FROM AUTHOR]