1. γ-Glutamylcysteine rescues mice from TNBS-driven inflammatory bowel disease through regulating macrophages polarization.
- Author
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Zhou, Jinyi, Yan, Xintong, Bi, Xiaowen, Lu, Shuai, Liu, Xianli, Yang, Chen, Shi, Yingying, Luo, Lan, and Yin, Zhimin
- Subjects
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INFLAMMATORY bowel diseases , *PROTEIN expression , *MACROPHAGES , *WEIGHT loss , *MICE - Abstract
Objective: To explore the molecular mechanism of γ-glutamylcysteine (γ-GC) in response to inflammation in vivo and in vitro on regulating the polarization of macrophages. Methods: The expressions of gene or protein were assessed by qPCR and Western blot assays, respectively. Cell viability was investigated by CCK-8 assay. Eight-week-old male BALB/c mice were established to examine the therapeutic effects of γ-GC in vivo. The release of TNF-α and IL-4 was determined by ELISA assay. Macrophages polarization was identified by flow cytometry assay. Results: Our data showed that γ-GC treatment significantly improved the survival, weight loss, and colon tissue damage of IBD mice. Furthermore, we established M1- and M2-polarized macrophages, respectively, and our findings provided evidence that γ-GC switched M1/M2-polarized macrophages through activating AMPK/SIRT1 axis and inhibiting inflammation-related signaling pathway. Conclusion: Collectively, both in vivo and in vitro experiments suggested that γ-GC has the potential to become a promising novel therapeutic dipeptide for the treatment of IBD, which provide new ideas for the treatment of inflammatory diseases in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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