1. Selenoprotein I is essential for murine embryogenesis.
- Author
-
Avery JC, Yamazaki Y, Hoffmann FW, Folgelgren B, and Hoffmann PR
- Subjects
- Animals, Animals, Newborn, Blastocyst ultrastructure, Embryo Implantation, Embryo Loss genetics, Embryo Loss pathology, Embryonic Development, Ethanolaminephosphotransferase, Female, Gene Deletion, Homozygote, Male, Mice genetics, Mice, Inbred C57BL, Mice, Knockout, Pregnancy, Blastocyst pathology, Gene Expression Regulation, Developmental, Mice embryology
- Abstract
Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI
-/+ mating pairs were used to generate global KO offspring. Of 323 weanling pups, no homozygous KO genotypes were found. E6.5-E18.5 embryos (165 total) were genotyped, and only two E18.5 KO embryos were detected with no discernable anatomical defects. To screen embryos prior to uterine implantation that occurs ~ E6, blastocyst embryos (E3.5-E4.4) were flushed from uteruses of pregnant females and analyzed for morphology and genotype. KO embryos were detected in 5 of 6 pregnant females, and 7 of the 32 genotyped embryos were found to be SELENOI KO that exhibited no overt pathological features. Overall, these results demonstrate that, except for rare cases (2/490 = 0.4%), global SELENOI deletion leads to early embryonic lethality., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2020
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