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Your search keyword '"Pravtcheva D"' showing total 4 results
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4 results on '"Pravtcheva D"'

1. The gene encoding peripheral myelin protein zero is located on mouse chromosome 1.

Author

Kuhn R, Pravtcheva D, Ruddle F, and Lemke G

Subjects
Animals, Blotting, Southern, Cell Line, Cricetinae genetics, Hybrid Cells, Myelin P0 Protein, Chromosome Mapping, Mice genetics, Myelin Proteins genetics
Abstract

We have used somatic cell hybrids to map the gene encoding protein zero (P0), the major structural protein of peripheral myelin. Analysis of Southern blots of DNA obtained from these hybrids allows us to unambiguously assign the P0 gene to mouse chromosome 1. This observation indicates that mutations in the P0 gene do not account for Trembler, a chromosome 11 mutation that specifically affects myelination in the peripheral nervous system.

Published
1990

2. Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains.

Author

D'Eustachio P, Pravtcheva D, Marcu K, and Ruddle FH

Subjects
Animals, Cells, Cultured, Chromosome Mapping, Genetic Linkage, Hybrid Cells immunology, Immunoglobulin alpha-Chains genetics, Immunoglobulin gamma-Chains genetics, Immunoglobulin mu-Chains genetics, Nucleic Acid Hybridization, Genes, Immunoglobulin Constant Regions genetics, Immunoglobulin Heavy Chains genetics, Immunoglobulins genetics, Mice genetics
Abstract

To determine the chromosomal location of mouse immunoglobulin heavy chain structural genes unambiguously, a panel of somatic cell hybrids was scored for the presence of DNA sequences homologous to gamma 2b-, mu-, and alpha-heavy chain-constant region DNA probe molecules. The hybrids, formed between mouse and hamster cells, contained various combinations of mouse chromosomes plus a full set of hamster chromosomes. Hybrids that retained mouse chromosome 12 reacted with the probes, whereas hybrids that had lost the chromosome, or its distal half, failed to react. These results indicate that structural genes for the gamma 2b-, mu-, and alpha-heavy chain-constant regions map to the distal half of this chromosome.

Published
1980
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3. Localization of the cryptdin locus on mouse chromosome 8.

Author

Ouellette AJ, Pravtcheva D, Ruddle FH, and James M

Subjects
Adrenocorticotropic Hormone antagonists & inhibitors, Animals, Blotting, Southern, Chromosome Mapping, Defensins, Hybrid Cells, In Vitro Techniques, Karyotyping, Mice, Inbred Strains, Polymorphism, Restriction Fragment Length, Blood Proteins genetics, Mice genetics, Protein Precursors genetics, Proteins genetics
Abstract

Cryptdin is a defensin-related peptide, and its mRNA accumulates to high abundance in epithelial cells of intestinal crypts beginning in the second week of postnatal development. The cryptdin (Defcr) locus was assigned to mouse chromosome 8 by Southern blotting of DNAs from mouse/hamster somatic hybrid cell lines. Analysis of somatic hybrid DNAs for mouse-specific restriction fragments showed zero discordance and perfect concordance with chromosome 8. The Defcr locus was localized on chromosome 8 by analysis of DNAs from recombinant inbred (RI) strains of mice after identification of three potential Defcr alleles based on restriction fragment length polymorphisms (RFLPs) in inbred strains. The strain distribution patterns of the Defcr locus were compared with those of chromosome 8 markers in five panels of RI strains. Analysis of cosegregation of Defcr with xenotropic proviral locus Xmv-26 and additional loci confirmed the chromosomal assignment and showed that Defcr is on proximal chromosome 8 within approximately 6 (1.3 to 21.3) cM of Xmv-26. The mouse Defcr locus and the human defensin gene(s) located on chromosome 8p23 appear to map to homologous regions.

Published
1989
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4. A novel 6:10 chromosomal translocation in the murine plasmacytoma NS-1

Author

Joan L. Klotz, L. Hood, Roger M. Perlmutter, Pravtcheva D, and Ruddle F

Subjects
Genetics, Multidisciplinary, Oncogene, Chromosome Mapping, Chromosomal translocation, Oncogenes, Transfection, Biology, medicine.disease, medicine.disease_cause, Molecular biology, Translocation, Genetic, Cell Line, Immunoglobulin kappa-Chains, Mice, Cell Transformation, Neoplastic, Cell culture, medicine, Animals, Neoplasm, Plasmacytoma, Immunoglobulin Constant Regions, Carcinogenesis, Gene
Abstract

Specific chromosomal abnormalities are regularly associated with many marine and human malignancies1,2. In particular, the majority of murine plasmacytomas and human Burkitt's lymphomas contain a characteristic translocation which results in the juxtaposition of a cellular oncogene, c-myc, with the immunoglobulin heavy-chain gene locus3–7, and this rearranged c-myc directs the synthesis of qualitatively and quantitatively abnormal transcripts which may have an aetiological role in the development of the transformed state in lymphoid malignancies8,9. Similarly, rearrangement and abnormal expression of c-myb (ref. 10) and c-mos (ref. 11) has been reported in other murine lymphoid tumours. Here we describe a novel 6:10 chromosomal translocation in the murine plasmacytoma cell line NS-1 which juxtaposes the immunoglobulin Cκ gene with a single-copy sequence of unknown function. The NS-1 plasmacytoma is a frequently used fusion partner in hybridoma production12 and is known to contain a rearranged c-myc gene4,13 and a genetic element which transforms normal mouse fibroblasts in DNA-mediated transfection assays14. We conclude that individual B-cell tumours may contain multiple chromosomal translocations perhaps relevant to oncogenesis.

Published
1984
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