6 results on '"Martin Fontecha, A"'
Search Results
2. Triggering of Murine NK Cells by CD40 and CD86 (B7-2)
- Author
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Martin-Fontecha, A., Assarsson, E., ennio carbone, Karre, K., Ljunggren, Hg, MARTIN-FONTECHA, A, Assarsson, E, Carbone, E, Karre, K, and Ljunggren, Hg.
- Subjects
Cytotoxicity, Immunologic ,Mice, Inbred BALB C ,Membrane Glycoproteins ,T-Lymphocytes ,CD40 Ligand ,Immunology ,Dendritic Cells ,Recombinant Proteins ,Killer Cells, Natural ,Mice, Inbred C57BL ,Mice ,Phenotype ,CD28 Antigens ,Antigens, CD ,Tumor Cells, Cultured ,Animals ,Interleukin-2 ,Immunology and Allergy ,B7-2 Antigen ,Interferons ,CD40 Antigens ,Spleen - Abstract
NK cell-mediated cytotoxicity is regulated by both triggering and inhibitory signals. The interaction between MHC class I molecules expressed on target cells and specific MHC class I-binding receptors expressed by NK cells generally leads to inhibition of lysis. We have shown recently that CD80 (B7-1) in mice and CD40 in humans trigger NK cell-mediated cytotoxicity in vitro. In the present study, we show that murine CD40 and CD86 (B7-2) trigger murine NK cell-mediated cytotoxicity in vitro when expressed on tumor cells. Preincubation of the transfected cell lines with anti-CD40 F(ab′)2 fragments or cytolytic T lymphocyte-associated Ag-4-Ig (CTLA-4-Ig) before the cytotoxic assay abolished the triggering effect. Furthermore, radiolabeled CD40- and B7-2-expressing cells were rapidly eliminated in vivo in an NK cell-dependent manner. NK cells from CD40 ligand (CD40L)−/− or CD28−/− mice were triggered by tumor cells transfected with CD40 and B7-2, respectively, and these transfectants were rapidly eliminated in vivo when inoculated into CD40L−/− and CD28−/− mice. This suggests that the CD40 and B7-2 molecules can interact with receptors on NK cells other than CD40L and CD28, respectively, and that these may account for some of the reactivities observed in the present study. Collectively, these data demonstrate that 1) costimulatory molecules, other than B7-1, can modulate NK cell responses in vitro, 2) they can also affect NK cell-dependent responses in vivo, and 3) parts of these reactions are independent of CD28 and CD40L.
- Published
- 1999
3. Rejection of a nonimmunogenic melanoma by vaccination with natural melanoma peptides on engineered antigen-presenting cells
- Author
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Bellone, M., Iezzi, G., Martin-Fontecha, A., Rivolta, L., Manfredi, A. A., Protti, M. P., Freschi, M., Paolo Dellabona, Casorati, G., Rugarli, C., Bellone, M, Iezzi, G, Martinfontecha, A, Rivolta, L, Manfredi, ANGELO ANDREA M. A., Protti, Mp, Freschi, M, Dellabona, P, Casorati, G, and Rugarli, C.
- Subjects
Graft Rejection ,Vaccines, Synthetic ,Immunology ,Melanoma, Experimental ,Antigen-Presenting Cells ,Cancer Vaccines ,Killer Cells, Natural ,Mice, Inbred C57BL ,Mice ,Antigens, Neoplasm ,B7-1 Antigen ,Animals ,Immunology and Allergy ,Female ,T-Lymphocytes, Cytotoxic - Abstract
Naturally processed peptides, obtained by acid extraction of tumor cells, contain Ags able to activate specific CTL in vitro. We recently reported that the nonprofessional APC, RMA-S, expressing the B7.1 molecule (RMA-S/B7), pulsed with naturally processed peptides from the nonimmunogenic B16F1 melanoma (B16F1a.e.) primed syngenic CD8+ T cells against the tumor in vitro. Here, we show the rejection of B16F1 melanoma by C57BL/6 mice after immunization with RMA-S/B7 cells pulsed with B16F1a.e. This response is critically dependent on both CD4+ and CD8+ cells, but not on NK cells. However, only CD8+ T cells exert anti-B16F1 cytolitic activity in vitro. Moreover, RMA-S/B7 cells pulsed with B16F1a.e. can be used to prevent the growth of 24-h preestablished melanomas. These results may have important implications for the clinical use of natural peptide fractions of tumor cells as therapeutic cancer vaccines.
- Published
- 1997
4. Apoptosis-dependent subversion of the T-lymphocyte epitope hierarchy in lymphoma cells
- Author
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Castiglioni, P., Martin-Fontecha, A., Milan, G., Tomajer, V., Fulvio Magni, Michaelsson, J., Rugarli, C., Rosato, A., Bellone, M., Castiglioni, P, Martin Fontecha, A, Milan, G, Tomajer, V, Magni, F, Michaelsson, J, Rugarli, C, Rosato, A, and Bellone, M
- Subjects
Lymphoma ,Thymoma ,Animal ,Immunodominant Epitopes ,Apoptosi ,Epitopes, T-Lymphocyte ,Gene Products, gag ,Apoptosis ,Rauscher Viru ,Lymphocyte Activation ,Caspase ,BIO/10 - BIOCHIMICA ,Cancer Vaccines ,Rauscher Virus ,Mice, Inbred C57BL ,Mice ,Caspases ,Animals ,Female ,Immunodominant Epitope ,Cancer Vaccine ,T-Lymphocytes, Cytotoxic - Abstract
Tumor cells undergoing programmed death are an attractive source of tumor-associated antigens, and evidences are available for their therapeutic efficacy in vivo when used either alone or in association with dendritic cells. However, little is known about the specificity of the immune response induced by such antigen formulation. Indeed, activation of specific proteases during apoptosis may influence the cytoplasmic degradation of proteins and the generation of CTL epitopes. We show here that on injection of C57BL/6 mice either with RMA lymphoma cells induced to apoptosis or bone marrow-derived dendritic cells pulsed with apoptotic RMA cells, a specific and protective CTL response is induced, which, however, is not directed against the immunodominant CTL epitope gag(85-93). Lack of in vivo expansion of gag(85-93)-specific CTL in vaccinated mice is attributable to the apoptosis-dependent loss of gag(85-93) in dying tumor cells. Indeed, we found loss of gag(85-93) in RMA, MBL-2, and EL-4G+ lymphoma cells, which share gag(85-93) as an immunodominant CTL epitope, induced to apoptosis by UV irradiation, mitomycin C, doxorubicin, or daunorubicin. This phenomenon appears to be caspase-dependent, because caspase inhibition by N-benzyloxycarbonyl-Val-Ala-asp-fluoromethylketone prevents apoptosis of lymphoma cells and loss of gag(85-93). Therefore, subversion of the epitope hierarchy in apoptotic tumor cells might be relevant in the induction of tumor-specific T-lymphocyte responses.
5. The immunogenicity of experimental tumors is strongly biased by the expression of dominant viral cytotoxic T-lymphocyte epitopes
- Author
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Iezzi, G., Rivolta, L., Ronchetti, A., Martin-Fontecha, A., Rosato, A., Protti, M. P., Sabbadini, M. G., and Matteo Bellone
- Subjects
Leukemia, Experimental ,Lymphoma ,Thymoma ,Immunodominant Epitopes ,Receptors, Antigen, T-Cell, alpha-beta ,Vaccination ,Cytotoxicity Tests, Immunologic ,Immunohistochemistry ,Rauscher Virus ,Friend murine leukemia virus ,Leukemia Virus, Murine ,Mice, Inbred C57BL ,Mice ,Tumor Virus Infections ,Tumor Cells, Cultured ,Animals ,Female ,Moloney murine leukemia virus ,Antigens, Viral ,Neoplasm Transplantation - Abstract
The immunogenic Friend-Moloney-Rauscher (FMR) virus-induced tumors have been used extensively to clarify the cellular and molecular mechanisms responsible for tumor rejection and to develop immunotherapeutic strategies. We characterize here the trimolecular complex MHC class I-antigenic determinant-T cell receptor involved in the induction of a protective CTL response against the RMA thymoma. This complex is mainly composed by the D(b) molecule interacting with a Rauscher virus antigen (Ag) determinant and the Vbeta5+ T cell receptor. We also show that the chemically induced EL-4 thymoma acquires the susceptibility to recognition by anti-RMA CTLs and the ability to elicit a protective anti-RMA CTL response only upon infection by a virus of the FMR family and that RMA and FMR virus infected EL-4 cells share tumor-associated Ag. The data strongly support the hypothesis that the high immunogenicity of virus-induced or infected tumors is determined by the expression of immunodominant virus-encoded Ag. The demonstration of a different outcome in the immune responses elicited in the presence or in the absence of viral Ag further open the contention of the molecular requirements for immunogenicity and should stimulate a more careful revision of unexpected cross-reactivity among tumors.
6. Activation of the sympathetic nervous system mediates hypophagic and anxiety-like effects of CB1 receptor blockade
- Author
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Carmelo Quarta, D. S. de Vega, Martin Häring, Pierre Cardinal, Mar Martín-Fontecha, Manuel Guzmán, E. Binder, Thierry Leste-Lasserre, Anna Delamarre, Giovanni Marsicano, Francis Chaouloff, Uberto Pagotto, Edgar Soria-Gomez, Luigi Bellocchio, Astrid Cannich, Mathilde Metna-Laurent, Dusan Bartsch, Beat Lutz, Krisztina Monory, Daniela Cota, L. Bellocchio, E. Soria-Gomez, C. Quarta, M. Metna-Laurent, P. Cardinal, E. Binder, A. Cannich, A. Delamarre, M. Haring, M. Martin-Fontecha, D. Vega, T. Leste-Lasserre, D. Bartsch, K. Monory, B. Lutz, F. Chaouloff, U. Pagotto, M. Guzman, D. Cota, and G. Marsicano
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medicine.medical_specialty ,Sympathetic nervous system ,Sympathetic Nervous System ,Cannabinoid receptor ,medicine.medical_treatment ,Anxiety ,Biology ,Neurotransmission ,Synaptic Transmission ,Feeding and Eating Disorders ,Mice ,03 medical and health sciences ,Glutamatergic ,0302 clinical medicine ,Receptor, Cannabinoid, CB1 ,Rimonabant ,Internal medicine ,fear and anxiety, sympathetic system ,medicine ,Animals ,Receptor ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,Multidisciplinary ,Appetite Regulation ,Brain ,Biological Sciences ,3. Good health ,Blockade ,Gastrointestinal Tract ,Endocrinology ,medicine.anatomical_structure ,nervous system ,lipids (amino acids, peptides, and proteins) ,Cannabinoid ,Neuroscience ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Complex interactions between periphery and the brain regulate food intake in mammals. Cannabinoid type-1 (CB 1 ) receptor antagonists are potent hypophagic agents, but the sites where this acute action is exerted and the underlying mechanisms are not fully elucidated. To dissect the mechanisms underlying the hypophagic effect of CB 1 receptor blockade, we combined the acute injection of the CB 1 receptor antagonist rimonabant with the use of conditional CB 1 -knockout mice, as well as with pharmacological modulation of different central and peripheral circuits. Fasting/refeeding experiments revealed that CB 1 receptor signaling in many specific brain neurons is dispensable for the acute hypophagic effects of rimonabant. CB 1 receptor antagonist-induced hypophagia was fully abolished by peripheral blockade of β-adrenergic transmission, suggesting that this effect is mediated by increased activity of the sympathetic nervous system. Consistently, we found that rimonabant increases gastrointestinal metabolism via increased peripheral β-adrenergic receptor signaling in peripheral organs, including the gastrointestinal tract. Blockade of both visceral afferents and glutamatergic transmission in the nucleus tractus solitarii abolished rimonabant-induced hypophagia. Importantly, these mechanisms were specifically triggered by lipid-deprivation, revealing a nutrient-specific component acutely regulated by CB 1 receptor blockade. Finally, peripheral blockade of sympathetic neurotransmission also blunted central effects of CB 1 receptor blockade, such as fear responses and anxiety-like behaviors. These data demonstrate that, independently of their site of origin, important effects of CB 1 receptor blockade are expressed via activation of peripheral sympathetic activity. Thus, CB 1 receptors modulate bidirectional circuits between the periphery and the brain to regulate feeding and other behaviors.
- Published
- 2013
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