1. Modulation of Immune Response to Chlamydia muridarum by Host miR-135a
- Author
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Lane K. Christenson, James P. Chambers, Bernard P. Arulanandam, Xingguo Cheng, Jieh Juen Yu, Jonathon Keck, M. N. Guentzel, and Rishein Gupta
- Subjects
0301 basic medicine ,Microbiology (medical) ,Chemokine ,Chlamydia muridarum ,cell migration ,Immunology ,lcsh:QR1-502 ,CXCR3 ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,Chemokine receptor ,Mice ,0302 clinical medicine ,Immune system ,Cellular and Infection Microbiology ,Immunity ,CXCL10 ,Animals ,dendritic cells ,Chlamydia ,miR135a ,biology ,microRNA ,Cell migration ,Chlamydia Infections ,Brief Research Report ,biology.organism_classification ,Cell biology ,MicroRNAs ,030104 developmental biology ,Infectious Diseases ,030220 oncology & carcinogenesis ,biology.protein ,Chemokines - Abstract
Previously, our laboratory established the role of small, noncoding RNA species, i.e., microRNA (miRNA) including miR-135a in anti-chlamydial immunity in infected hosts. We report here chlamydial infection results in decreased miR-135a expression in mouse genital tissue and a fibroblast cell line. Several chemokine and chemokine receptor genes (including CXCL10, CCR5) associated with chlamydial pathogenesis were identified in silico to contain putative miR-135a binding sequence(s) in the 3’ untranslated region. The role of miR-135a in the host immune response was investigated using exogenous miR-135a mimic to restore the immune phenotype associated with decreased miR-135a following Chlamydia muridarum (Cm) infection. We observed miR-135a regulation of Cm-primed bone marrow derived dendritic cells (BMDC) via activation of Cm-immune CD4+ T cells for clonal expansion and CCR5 expression. Using a transwell cell migration assay, we explore the role of miR-135a in regulation of genital tract CXCL10 expression and recruitment of CXCR3+ CD4+ T cells via the CXCL10/CXCR3 axis. Collectively, data reported here support miR-135a affecting multiple cellular processes in response to chlamydial infection.
- Published
- 2021