1. Nanotailored hyaluronic acid modified methylcellulose as an injectable scaffold with enhanced physico-rheological and biological aspects.
- Author
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Das B, Basu A, Maji S, Dutta K, Dewan M, Adhikary A, Maiti TK, and Chattopadhyay D
- Subjects
- Cell Line, Cell Movement drug effects, Cell Proliferation drug effects, Erythrocytes drug effects, Fibroblasts drug effects, Fibroblasts physiology, Hemolysis drug effects, Humans, Rheology, Wound Healing drug effects, Biocompatible Materials chemistry, Biocompatible Materials pharmacology, Hyaluronic Acid chemistry, Hyaluronic Acid pharmacology, Hydrogels chemistry, Hydrogels pharmacology, Methylcellulose chemistry, Methylcellulose pharmacology, Tissue Scaffolds
- Abstract
The collaborative endeavor in tissue engineering is to fabricate a bio-mimetic extracellular matrix to assist tissue regeneration. Thus, a novel injectable tissue scaffold was fabricated by exploring nanotailored hyaluronic acid (nHA) and methylcellulose (MC) (nHAMC) along with pristine HA based MC scaffold (HAMC). nHA with particle size ∼22 ± 5.3 nm were obtained and nHAMC displayed a honeycomb-like 3D microporous architecture. Nano-HA bestowed better gel strength, physico-rheological and biological properties than HA. It creditably reduced the high content of salt to reduce the gelation temperature of MC. The properties ameliorated with increased in-corporation of nano-HA. The addition of salt showed more prominent effect on gelation temperature of nHAMC than in HAMC; and salting-out effect was dependent on nHA/HA content. Biocompatible nHAMC assisted adequate cell adherence and proliferation with more extended protrusions with better migration rate than control. Thus, biomodulatory effect of nanotailored glycosaminoglycan could be asserted to design an efficient thermo-responsive scaffold., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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