7 results on '"Booij, Linda"'
Search Results
2. DNA methylation of the serotonin transporter gene (SLC6A4) is associated with brain function involved in processing emotional stimuli
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Frodl, Thomas, Szyf, Moshe, Carballedo, Angela, Ly, Victoria, Dymov, Sergiy, Vaisheva, Farida, Morris, Derek, Fahey, Ciara, Meaney, James, Gill, Michael, and Booij, Linda
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Methylation ,Depression, Mental ,Serotonin ,Health ,Psychology and mental health - Abstract
Background: The aim of the present study was to investigate the association of fMRI blood oxygen-level dependent (BOLD) reactivity with the level of epigenetic methylation of SLC6A4 in blood DNA from a sample of healthy participants and patients with major depressive disorder (MDD). Methods: We investigated patients with MDD and healthy controls using fMRI and an emotional attention-shifting task. We assessed site-specific DNA methylation of a previously characterized SLC6A4 region in peripheral blood DNA using pyrosequencing. Results: Our study involved 25 patients with MDD and 35 healthy controls. Activation in the anterior insula elicited by negative emotional content was significantly positively associated with the degree of SLC6A4 methylation. Significantly negative associations were observed between activation in the posterior insula and the degree of SLC6A4 methylation when judging the geometry of pictures after seeing negative in contrast to positive emotional stimuli. Healthy controls with a high degree of SLC6A4 methylation depicted significantly more activity elicited by positive stimuli in limbic regions and more activity elicited by negative stimuli in limbic as well as cognitive control regions than those with a low degree of SLC6A4 methylation. Limitations: It is impossible to measure methylation directly in the brain and thus we assessed peripheral methylation of SLC6A4. Since the association was cross-sectional, no conclusion about cause and effect can be drawn. Conclusion: Our study provides further support to the hypothesis that particular DNA methylation states that are associated with brain function during emotion processing are detectable in the periphery., Introduction Childhood adversity, such as childhood maltreatment, plays an important role in a number of multifactorial mental disorders. Recent studies reveal that certain aspects of stress-related mental disorders result from [...]
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- 2015
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3. DNA methylation in people with anorexia nervosa: Epigenome-wide patterns in actively ill, long-term remitted, and healthy-eater women.
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Steiger, Howard, Booij, Linda, Thaler, Lea, St-Hilaire, Annie, Israël, Mimi, Casey, Kevin F., Oliverio, Stephanie, Crescenzi, Olivia, Lee, Viveca, Turecki, Gustavo, Joober, Ridha, Szyf, Moshe, and Breton, Édith
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DNA methylation , *ANOREXIA nervosa , *METHYLATION , *EATING disorders , *GENE expression , *GENE mapping - Abstract
Recent studies have reported altered methylation levels at disorder-relevant DNA sites in people who are ill with Anorexia Nervosa (AN) compared to findings in people with no eating disorder (ED) or in whom AN has remitted. The preceding implies state-related influences upon gene expression in people with AN. This study further examined this notion. We measured genome-wide DNA methylation in 145 women with active AN, 49 showing stable one-year remission of AN, and 64 with no ED. Comparisons revealed 205 differentially methylated sites between active and no ED groups, and 162 differentially methylated sites between active and remitted groups (Q < 0.01). Probes tended to map onto genes relevant to psychiatric, metabolic and immune functions. Notably, several of the genes identified here as being differentially methylated in people with AN (e.g. SYNJ2, PRKAG2, STAT3, CSGALNACT1, NEGR1, NR1H3) have figured in previous studies on AN. Effects also associated illness chronicity and lower BMI with more pronounced DNA methylation alterations, and remission of AN with normalisation of DNA methylation. Findings corroborate earlier results suggesting reversible DNA methylation alterations in AN, and point to particular genes at which epigenetic mechanisms may act to shape AN phenomenology. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Methylation of the OXTR gene in women with anorexia nervosa: Relationship to social behavior.
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Thaler, Lea, Brassard, Sarah, Booij, Linda, Kahan, Esther, McGregor, Kevin, Labbe, Aurelie, Israel, Mimi, and Steiger, Howard
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PERSONALITY disorder diagnosis ,ANOREXIA nervosa ,CELL receptors ,GENETIC mutation ,OXYTOCIN ,SOCIAL skills ,WOMEN'S health ,DISEASE remission ,DNA methylation ,EPIGENOMICS ,EVALUATION - Abstract
DNA methylation allows for the environmental regulation of gene expression and is believed to link environmental stressors to psychiatric disorder phenotypes, such as anorexia nervosa (AN). The oxytocin receptor (OXTR) gene is epigenetically regulated, and studies have shown associations between OXTR and social behaviours in various samples, including women with AN. The present study examined differential levels of methylation at various CG sites of the OXTR gene in 69 women with active AN (AN‐Active), 21 in whom AN was in remission (AN‐Rem) and 35 with no eating disorder (NED). Within each group, we explored the correlation between methylation and measures of social behaviour such as insecure attachment and social avoidance. Hypermethylation of a number of CG sites was seen in AN‐Active participants as compared with AN‐Rem and NED participants. In the AN‐Rem sample, methylation at CG27501759 was significantly positively correlated with insecure attachment (r =.614, p =.003, permutation Q = 0.008) and social avoidance (r =.588, p =.005, permutation Q = 0.0184). Our results highlight differential methylation of the OXTR gene among women with AN, those in remission from AN, and those who never had AN and provide some evidence of associations between OXTR methylation and social behaviour in women remitted from AN. [ABSTRACT FROM AUTHOR]
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- 2020
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5. DNA methylation in individuals with anorexia nervosa and in matched normal-eater controls: A genome-wide study.
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Booij, Linda, Casey, Kevin F., Antunes, Juliana M., Szyf, Moshe, Joober, Ridha, Israël, Mimi, and Steiger, Howard
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AGE factors in disease , *ANALYSIS of variance , *ANOREXIA nervosa , *CHI-squared test , *COMPARATIVE studies , *STATISTICAL correlation , *MENTAL depression , *DNA , *GENETICS , *INTERVIEWING , *CLASSIFICATION of mental disorders , *METHYLATION , *PATH analysis (Statistics) , *POLYMERASE chain reaction , *QUESTIONNAIRES , *REGRESSION analysis , *RESEARCH funding , *SCALE analysis (Psychology) , *STATISTICS , *T-test (Statistics) , *DATA analysis , *BODY mass index , *RETROSPECTIVE studies - Abstract
ABSTRACT Objective Evidence associates anorexia nervosa (AN) with epigenetic alterations that could contribute to illness risk or entrenchment. We investigated the extent to which AN is associated with a distinct methylation profile compared to that seen in normal-eater women. Method Genome-wide methylation profiles, obtained using DNA from whole blood, were determined in 29 women currently ill with AN (10 with AN-restrictive type, 19 with AN-binge/purge type) and 15 normal-weight, normal-eater control women, using 450 K Illumina bead arrays. Results Regardless of type, AN patients showed higher and less-variable global methylation patterns than controls. False Discovery Rate corrected comparisons identified 14 probes that were hypermethylated in women with AN relative to levels obtained in normal-eater controls, representing genes thought to be associated with histone acetylation, RNA modification, cholesterol storage and lipid transport, and dopamine and glutamate signaling. Age of onset was significantly associated with differential methylation in gene pathways involved in development of the brain and spinal cord, while chronicity of illness was significantly linked to differential methylation in pathways involved with synaptogenesis, neurocognitive deficits, anxiety, altered social functioning, and bowel, kidney, liver and immune function. Discussion Although pre-existing differences cannot be ruled out, our findings are consistent with the idea of secondary alterations in methylation at genomic regions pertaining to social-emotional impairments and physical sequelae that are commonly seen in AN patients. Further investigation is needed to establish the clinical relevance of the affected genes in AN, and, importantly, reversibility of effects observed with nutritional rehabilitation and treatment. © 2015 Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:874-882) [ABSTRACT FROM AUTHOR]
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- 2015
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6. Le rôle de la méthylation de l’ADN dans les fonctions cérébrales fronto-limbiques et la réactivité au stress quotidien
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Verner, Marie-Pier V., Booij, Linda, and Séguin, Jean
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Cerveau ,Serotonin ,Psychopathologie ,Psychopathology ,Sérotonine ,Méthylation ,fMRI ,Brain ,Stress ,Methylation ,Imagerie cérébrale - Abstract
La sérotonine (5-HT) joue un rôle crucial dans l'étiologie des troubles mentaux comme la dépression majeure, les troubles de comportement et les troubles anxieux. Des études ont montré que des altérations précoces du système 5-HT peuvent potentiellement influencer le développement du cerveau et le fonctionnement du système fronto-limbique, engendrant des conséquences pour la régulation émotionnelle. Il existe aussi des évidences que le stress précoce peut affecter la méthylation de l'ADN résultant d'une altération de l'expression génique. Toutefois, le lien entre la méthylation de l'ADN et la réactivité comportementale à des facteurs de stress de la vie quotidienne est inconnu. La méthylation du gène transporteur 5-HT (SLC6A4) est d'un intérêt particulier, étant donné le rôle de SLC6A4 dans le développement du cerveau, les troubles mentaux et la régulation du stress. L'objectif de cette thèse est d'étudier l'association entre (1) les niveaux périphériques de méthylation de l'ADN dans le gène SLC6A4 et les réponses neurales aux stimuli émotionnels dans les circuits fronto-limbiques du cerveau, ainsi qu’entre (2) la méthylation périphérique de SLC6A4 et la réactivité comportementale au stress de la vie quotidienne. Nous explorons également l'association entre les réponses neuronales fronto-limbique à des stimuli émotionnels et la réactivité comportementale au stress de la vie quotidienne (3). À cette fin, vingt-deux personnes (11 femmes) d’âge moyen de 34,0 ans (SD : 1,5) avec différents niveaux de méthylation au gène SLC6A4 ont été recrutés à partir de deux études longitudinales. Les participants ont subi une analyse IRMf qui comprenait une tâche de traitement émotionnel. Un questionnaire en ligne sur la réactivité au stress quotidien de la vie a été réalisé pendant 5 jours consécutifs. Des analyses corrélationnelles et de régression ont été effectuées pour examiner les associations entre les variables primaires. Les résultats préliminaires de cette étude ont montré que la méthylation de l'ADN est associée à la désactivation significative du gyrus précentral et gyrus fusiforme respectivement face à des stimuli de peur et de tristesse. Aucune association significative n'a été observée entre les niveaux de méthylation et l'activation de l'amygdale. En outre, les scores obtenus aux variables de stress de la vie quotidienne tels que la détresse chronique ont été associées à la désactivation du précuneus et du cortex cingulaire postérieur face à la tristesse. Ces résultats suggèrent l'implication potentielle des processus épigénétiques dans l'activation cérébrale spécifique et la sensibilité au stress de la vie courante., Serotonin (5-HT) plays a crucial role in mental disorders etiology such as major depression, conduct disorders and anxiety disorders. Studies have shown that early alterations in the 5-HT system can potentially influence frontal-limbic brain development and functioning, with consequences for emotional regulation. There is also evidence that early stress can affect DNA methylation resulting in an alteration in gene expression; however the link between DNA methylation and behavioural reactivity to current daily life stressors is not known. DNA methylation in the 5-HT transporter (SLC6A4) is of particular interest, given the role of SLC6A4 in brain development, mental disorders and stress regulation. The aim of the present thesis is to study the association between (1) peripheral levels of DNA methylation inthe SLC6A4 gene and the neural responses to emotional stimuli in the frontal-limbic brain circuitry (2) peripheral SLC6A4 methylation and behavioural reactivity to daily life stress. We also explore the association between frontal-limbic neural responses to emotional stimuli and behavioural reactivity to daily life stress (3). To this end, twenty-two individuals (11 females) with mean age of 34.0 years old (SD: 1.5) with various levels of SLC6A4 methylation gene were recruited from two longitudinal studies. Participants underwent an fMRI scan that included an emotional processing task. An online questionnaire on daily life stress reactivity was performed during 5 consecutive days. Correlations and regressions analyses were performed to investigate the associations among the primary variables. Results of this pilot study showed that DNA methylation is associated with the significant deactivation of the precentral gyrus and fusiform gyrus when respectively facing fearful and sad stimuli. No significant association was observed between levels of methylation and amygdala activation. Also, obtained scores todaily life stress variables such as chronic distress were associated with the deactivation of the precuneus and posterior cingulate cortex when facing sadness. Those results suggest the potential implication of epigenetic processes in specific brain activation and daily life stress sensitivity.
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- 2016
7. F135. FKBP5 Methylation is Associated With Frontal-Limbic Brain Structure and Function in Depressed Adolescents and Healthy Controls.
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Chiarella, Julian, Pomares, Florence, Tozzi, Leonardo, Frodl, Thomas, Khalid-Khan, Sarosh, Schumann, Lyndall, Nguyen, Tuong-Vi, Yu, Wei-Jo, Szyf, Moshe, Nemoda, Zsofia, and Booij, Linda
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DEPRESSION in adolescence , *METHYLATION , *DNA methylation - Published
- 2018
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