1. Effect of Chronic Exposure to Methomyl on Tissue Damage and Apoptosis in Testis of Tilapia (Oreochromis niloticus) and Recovery Pattern.
- Author
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Meng SL, Liu T, Chen X, Qiu LP, Hu GD, Song C, Fan L, Zheng Y, Chen JZ, and Xu P
- Subjects
- Animals, Dose-Response Relationship, Drug, Male, No-Observed-Adverse-Effect Level, Apoptosis drug effects, Cichlids, Methomyl toxicity, Testis drug effects, Water Pollutants, Chemical toxicity
- Abstract
Tilapia were exposed to 0, 0.2, 2, 20, 200 µg/L methomyl for 30 days, and then transferred to methomyl-free water for 18 days. Caspase-8 in serum, apoptosis rate, microstructure and ultra-microstructure of testis were checked after methomyl exposure and at 18 days after transferring to methomyl-free water. There were no significant changes in Caspase-8 activity, apoptosis rate, and tissue structure in testis exposed to 0.2 and 2 µg/L compared with control. However, when tilapia exposed to 20 and 200 µg/L, the Caspase-8 activity and apoptosis rate were induced significantly, and tissue damage happened compared with the control. Thus it would appear 2 µg/L methomyl might be considered as the no observed adverse effect level. Recovery data showed that the effects produced by lower concentration of 20 µg/L were reversible but not at the higher 200 µg/L concentration.
- Published
- 2019
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