Search

A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for estimation of Ethacridine lactate in pharmaceutical formulation. The method was carried out on a Qualisil RP C-18 (250 mm x 4.6 mm, 5 µm) column with a mobile phase consisting of methanol: water (60:40 v/v), pH adjusted to 2.8 with orthophosphoric acid and low rate of 1.0 mL/min. Detection was carried out at 271 nm. The retention time for ethacridine lactate was found to be 4.41 min. The ethacridine lactate followed linearity in the concentration range of 2- 12 µg/mL (r² = 0.9980). The amount of the drug estimated by proposed method was found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.11 and 0.33 µg. The proposed method can be used for routine analysis of ethacridine lactate in bulk drug and pharmaceutical formulation. [ABSTRACT FROM AUTHOR]

A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of paracetamol and valdecoxib in tablet formulation. The separation was achieved by Luna C18 column and methanol: phosphate buffer pH 3.5 (60:40 v/v) as eluent, at a flow rate of 1.0 ml/min. Detection was carried out at 242 nm. Etoricoxib was used as an internal standard. The retention time of PAR and VAL was found to be 3.01 and 8.51 min, respectively. The method was validated for linearity, accuracy and precision. Linearity for paracetamol and valdecoxib were in the range 25-150 µg/ml and 1-6 µg/ml, respectively. The developed method was found to be accurate, precise, and selective for simultaneous estimation of paracetamol and valdecoxib in tablets. [ABSTRACT FROM AUTHOR]

A simple, accurate, economical and reproducible procedure for simultaneous estimation of atorvastatin calcium and ezetimibe in their tablet dosage form has been developed using, Q-absorbance equation. The method involves, the absorbance measurement at 235.5 nm (iso-absorptive point) and 246.0 nm (λmax of atorvastatin calcium), in methanol. Both the drugs, obey Beer-Lambert's law in the concentration range of 5-25 µg/ml. The results of analysis have been validated statistically and by recovery studies. [ABSTRACT FROM AUTHOR]