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This experiment reports an energy-saving, cost-effective and environmental-friendly method to recover energy from high-cellulose waste: anaerobic digestion (AD) by extending hydraulic residence time (HRT) from 50d to 70d with ultra-high organic loading rate (OLR) of 2.28-2.80 g TS·L -1  d -1 . The results indicate that biogas yield per VS and methane yield per VS increase with the extended HRT, with a maximum increase of up to 67.9%, while both yields decrease as OLR increases. The volumetric gas production (VGP) and volumetric methane production (VMP) improve by 20.2-37.3% when HRT is extended to 57 days and OLR is 2.80 g TS·L -1  d -1 , reaching a peak at this point. As the biogas production capacity of the two-stage anaerobic digestion reaches its peak, the gap between the one-stage system and the two-stage system decreases from 23% to 7% under the same conditions. This demonstrates that optimizing HRT and OLR not only enhance the gas production efficiency of the AD system but also reduce the gas production disparity between single-stage and two-stage systems, thus serving as a cost-effective method for engineering operations. Microbial community analysis of each system reveals that extending HRT increases the abundance and diversity of microbial communities, while changes in HRT and OLR result in significant shifts in the distribution of methanogens. Through reasonable regulation of HRT and OLR, a balance can be found between the full degradation of organic matter and the system load, so as to maximize biogas production and efficiency., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Biogas offers significant benefits as a renewable energy source, contributing to decarbonization, waste management, and economic development. This comprehensive review examines the historical, technological, economic, and global aspects of biomethane production, focusing on the key players such as China, the European Union, and North America, and associated opportunities and challenges as well as future prospects from an Australia perspective. The review begins with an introduction to biogas, detailing its composition, feedstock sources, historical development, and anaerobic digestion (AD) process. Subsequently, it delves into major biomethane production technologies, including physicochemical absorption, high-pressure water scrubbing (HPWS), amine scrubbing (AS), pressure swing adsorption (PSA), membrane permeation/separation (MP), and other technologies including organic solvent scrubbing and cryogenic separation. The study also discusses general guidelines of techno-economic assessments (TEAs) regarding biomethane production, outlining the methodologies, inventory analysis, environmental life cycle assessment (LCA), and estimated production costs. Challenges and opportunities of biogas utilization in Australia are explored, highlighting and referencing global projections, polarization in production approaches, circularity in waste management, and specific considerations for Australia. The review concludes discussing future perspectives for biomethane, emphasizing the importance of technological advancements, policy support, and investment in realizing its full potential for sustainable energy and waste management solutions., (© 2024 The Authors. ChemSusChem published by Wiley-VCH GmbH.)

High ammonia concentrations in anaerobic degradation systems cause volatile fatty acid accumulation and reduced methane yield, which often derive from restricted activity of syntrophic acid-oxidising bacteria and hydrogenotrophic methanogens. Inclusion of additives that facilitate the electron transfer or increase cell proximity of syntrophic species by flocculation can be a suitable strategy to counteract these problems, but its actual impact on syntrophic interactions has yet to be determined. In this study, microbial cultivation and molecular and microscopic analysis were performed to evaluate the impact of conductive (graphene, iron oxide) and non-conductive (zeolite) additives on the degradation rate of acetate and propionate to methane by highly enriched ammonia-tolerant syntrophic cultures derived from a biogas process. All additives had a low impact on the lag phase but resulted in a higher rate of acetate (except graphene) and propionate degradation. The syntrophic bacteria 'Candidatus Syntrophopropionicum ammoniitolerans', Syntrophaceticus schinkii and a novel hydrogenotrophic methanogen were found in higher relative abundance and higher gene copy numbers in flocculating communities than in planktonic communities in the cultures, indicating benefits to syntrophs of living in close proximity to their cooperating partner. Microscopy and element analysis showed precipitation of phosphates and biofilm formation in all batches except on the graphene batches, possibly enhancing the rate of acetate and propionate degradation. Overall, the concordance of responses observed in both acetate- and propionate-fed cultures highlight the suitability of the addition of iron oxide or zeolites to enhance acid conversion to methane in high-ammonia biogas processes. KEY POINTS: • All additives promoted acetate (except graphene) and propionate degradation. • A preference for floc formation by ammonia-tolerant syntrophs was revealed. • Microbes colonised the surfaces of iron oxide and zeolite, but not graphene., (© 2024. The Author(s).)

Although two-stage anaerobic digestion (TSAD) technology has been investigated, the mechanisms regarding the impact of acidogenic off-gas (AOG) on successive methane production have not been well addressed. In this study, a novel TSAD system was designed. Food waste, as the main substrate, was co-digested with chicken manure and corn straw. The acidogenic gas beyond atmospheric pressure was introduced into the bottom of the methanogenesis reactor through a stainless steel diffuser. Results showed the addition of AOG increased the methane yield from 435.2 to 597.1 mL/g VS in in successive methanogenesis stage, improved by 37.2 %, and increased the energy yield from 9.0 to 11.3 kJ/g VS substrate . However, the theoretical contribution of hydrogenotrophic methanogenesis using H 2 contained in AOG was only 15.2 % of the increased methane yield. After the addition of AOG, the decreased levels of ammonia nitrogen and butyrate indicate that the stability of the AD system was improved. The electron transfer system and co-enzyme F420 activity were enhanced; however, the decrease in acetate kinase activity indicates aceticlastic methanogenesis may have been weakened. The microbial diversity and species richness were improved by the added AOG. Methanosarcina was more competitive than Methanothermobacter, enhancing the syntrophic effect. The relative abundance of protein degradation bacteria norank_f_Anaerolineaceae and lipid degradation bacteria Syntrophomonas was increased. Metabolite analysis confirmed that the addition of AOG promoted amino acid metabolism, the biosynthesis of other secondary metabolism and lipid metabolism. The improved degradation of recalcitrant organic components (lipids and proteins) in food waste was responsible for the increased methane yield. This study provides an in-depth understanding of the impact of AOG utilization on successive methane production and has practical implications for the treatment of food waste., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Anaerobic digestion can provide benefits not only from the perspective of renewable energy production but also in the form of fertilization effect and increased retention of C in soils after digestate application. This study consisted of two phases, where the first phase assessed the suitability of carbon-rich co-feedstocks for methane production via laboratory testing. The second phase assessed the balance and stability of C before and after anaerobic digestion by systematic digestate characterization, and by evaluating its carbon retention potential using a modeling approach. The results indicated that pyrolysis chars had a negligible effect on the methane production potential of cattle manure, while wheat straw expectedly increased methane production. Thus, a mixture of cattle manure and wheat straw was digested in pilot-scale leach-bed reactors and compared with undigested manure and straw. Although the total amount of C in the digestate was lower than in the untreated feedstocks, the digestion process stabilized C and was modeled to be more effective in retaining C in the soil than untreated cattle manure and wheat straw. In addition, digestion converted 23-27 % of the C into valuable methane, increasing the valorization of the total C in the feedstock. Considering anaerobic digestion processes as a strategy to optimize both carbon and nutrient valorization provides a more holistic approach to addressing climate change and improving soil health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Hydrothermal pretreatment has been proposed to enhance straw methane yield during anaerobic digestion recently. However, the combined effect of hydrothermal and organic acid pretreatment (HTOAP) needs further investigation. This study identified optimal pretreatment at 120 °C with 3 % acetic acid for 24 h by orthogonal design method. The HTOAP increased the reducing sugar content by destroying the lignocellulosic structure. A 79 % increment of methane production after HTOAP was observed compared to the untreated group. Microbial analysis showed that HTOAP enriched the relative abundance of lignocellulose-degraders, such as W5053, Thermanaerovibrio, Caldicoprobacter, as well as the syntrophic acetate oxidizing bacteria Syntrophaceticus. Moreover, Methanobacterium conducted hydrogenotrophic methanogenesis dominantly. Furthermore, the potential function analysis showed that HTOAP stimulated the expression of key enzymes in the hydrogenotrophic pathway, including carbon-monoxide dehydrogenase (EC 1.2.7.4) and coenzyme F420 hydrogenase (EC 1.12.98.1). This investigation illustrated the potential of HTOAP of rice straw to facilitate methane production., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Leach bed reactors (LBRs) are dry anaerobic systems that can handle feedstocks with high solid content, like chicken manure, with minimal water addition. In this study, the chicken manure was mixed with zeolite, a novel addition, and packed in the LBR to improve biogas production. The resulting leachate was then processed in a continuous stirred tank reactor (CSTR), where most of the methane was produced. The supernatant of the CSTR was returned to the LBR. The batch mode operation of the LBR led to a varying methane production rate (MPR) with a peak in the beginning of each batch cycle when the leachate was rich in organic matter. Comparing the MPR in both systems, the peaks in the zeolite system were higher and more acute than in the control system, which was under stress, as indicated by the acetate accumulation at 2328 mg L -1 . Moreover, the presence of zeolite in the LBR played a crucial role, increasing the overall methane yield from 0.142 (control experiment) to 0.171 NL CH 4 per g of volatile solids of chicken manure entering the system at a solid retention time of 14 d. Zeolite also improved the stability of the system. The ammonia concentration increased gradually due to the little water entering the system and reached 3220 mg L -1 (control system) and 2730 mg L -1 (zeolite system) at the end of the experiment. It seems that zeolite favored the accumulation of the ammonia at a lower rate (14.0 mg L -1 d -1 ) compared to the control experiment (17.3 mg L -1 d -1 ). The microbial analysis of the CSTR fed on the leachate from the LBR amended with zeolite showed a higher relative abundance of Methanosaeta (83.6%) compared to the control experiment (69.1%). Both CSTRs established significantly different bacterial profiles from the inoculum after 120 days of operation ( p < 0.05). Regarding the archaeal communities, there were no significant statistical differences between the CSTRs and the inoculum ( p > 0.05).

The ubiquitous use of volatile siloxanes in a myriad of product formulations has led to a widespread distribution of these persistent contaminants in both natural ecosystems and wastewater treatment plants. Microbial degradation under microaerobic conditions is a promising approach to mitigate D4 and D5 siloxanes while recovering energy in wastewater treatment plants. This study examined D4/D5 siloxanes biodegradation under both anaerobic and microaerobic conditions ( [Formula: see text]  = 0, 1, 3 %) using wastewater sludge. Results show that the use of microaeration in an otherwise strictly anaerobic environment significantly enhances siloxane conversion to methane. 16S rRNA gene sequencing identified potential degraders, including Clostridium lituseburense, Clostridium bifermentans and Synergistales species. Furthermore, chemical analysis suggested a stepwise siloxane conversion preceding methanogenesis under microaerobic conditions. This study demonstrates the feasibility of microaerobic siloxane biodegradation, laying groundwork for scalable removal technologies in wastewater treatment plants, ultimately highlighting the importance of using bio-based approaches in tackling persistent pollutants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study investigated biochemical methane potential (B 0 ) of manure residues and solid-liquid separation fractions from Australian dairies. This is important for country-specific sector emissions and biogas potential estimates. A range of samples were collected from 12 farms across 4 Australian states, and B 0 was measured. A first B 0 value for grazing dairy effluent is reported, at 161 L CH4 ·kg VS -1 . The B 0 of manure residues from intensive dairies with total mixed ration feeding was not significantly different, at 202 L CH4 ·kg VS with potential fugitive methane losses. Mechanical separation preserved B -1 , allowing organic matter diversion to reduce fugitive methane emissions. Cleaning method at a dairy significantly influenced residue total solids content, important for solid-liquid separation and selection of anaerobic digestion technology. Overall, B 0 with potential fugitive methane losses. Mechanical separation preserved B 0 methane per annum, with a total energy content of 2.8 petajoules·annum 0 for Australian dairy residues was estimated at 76.2 million m 3 N methane per annum, with a total energy content of 2.8 petajoules·annum -1 ., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

This study examined the effectiveness of introducing conductive carbon cloth into a pilot-scale high-solids anaerobic digestion (HSAD) system. Adding carbon cloth increased methane production by 22 % and improved the maximum methane production rate by 39 %. Microbial community characterization indicated a possible direct interspecies electron transfer-based syntrophic association among microbes. Using carbon cloth also enhanced microbial richness, diversity, and evenness. Carbon cloth effectively reduced the total abundance of antibiotic resistance genes (ARGs) by 44.6 %, mainly by inhibiting horizontal gene transfer, as shown by the significant decrease in the relative abundance of integron genes (particularly intl1). The multivariate analysis further demonstrated strong correlations of intl1 with most of the targeted ARGs. These findings suggest that carbon cloth amendment can promote efficient methane production and attenuate the spread of ARGs in HSAD systems., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The goal of neutrality in greenhouse gas emissions has intensified the search for renewable fuels. However, it is crucial to ensure sustainability of new technologies before proposing their implementation. This study proposes the use of life-cycle assessment (LCA) as an intermediary tool to identify critical hotspots in the exploration of hydrothermal pretreatment of lignocellulosic biomass, followed by biochemical methane potential assessment. Brewer s spent grain (BSG) was investigated, and laboratory-scale results were applied in an attributional assessment model with business-as-usual serving as the baseline. The LCA revealed that assumptions made in the lab could pose limitations. In Brazil, the two-stage co-digestion of pretreated hydrothermal BSG showed promising prospects, with a reduction to a new value of -54 kg CO 2 -eq Ton -1 BSG compared to 90 kg CO 2 -eq Ton -1 BSG in the business-as-usual scenario. Within the top ten global beer producing countries, only Brazil and Spain demonstrated potential for exploring this proposal., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Landfill gas (LFG) is related to the biochemical processes generating heat and releasing CH 4 , CO 2 , and other gases in lower concentrations, which result in environmental impacts and risk of local explosion. Thermal infrared imagery (TIR) is employed to detect CH 4 leakage as a risk control approach. However, the challenge for LFG leakage detection using TIR is establishing a relation between the gas flux and the ground temperature. This study evaluates the problem of a heated gas flowing through a porous medium column where the upward surface exchanges heat by radiation and convection to the environment. A heat transfer model that considers the upward LFG flow is proposed, and a sensibility analysis is developed to relate the flux to the ground temperature level in the condition of non-income solar radiation. An explicit equation to predict CH 4 fugitive flow as a function of temperature anomalies of the ground was presented for the first time. The results show that the predicted ground surface temperatures are consistent with the literature's experimental observations. Moreover, the model was complementarily applied to a Brazilian landfill, with in situ TIR measurements in an area with a slightly fractured cover. In this field observation, the predicted CH 4 flux was around 9025 g m -2 d -1 . Model limitations concerning the soil homogeneity, the transient variation of atmospheric conditions or local pressure, and soil temperature difference in low-flux conditions (related to TIR-cameras accuracy) require further validation. Results could help landfill monitoring in conditions of a high-temperature ground anomaly in dry seasons., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Despite the potential benefits of anaerobic digestion in the context of climate change and the need to move towards energy transition, there is a lot of resistance to biogas projects. Using a contingent valuation method, we test the extent to which the socio-economic characteristics of populations influence the environmental effort that people are willing to make for the deployment of biogas in a French region. Our results show that young people and people familiar with the biogas process are more inclined to develop biogas. We also highlight that the educational and location aspects should not be neglected in order to increase environmental effort and promote the adoption and development of biogas., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

A clean and sustainable energy source, biogas is widely accessible worldwide. The caloric value of biogas is related to its methane content, and therefore removal of other gases is essential for reaping the benefits of this cleaner resource. In contrast to other classical techniques, membrane technology is relatively new yet extremely promising for methane enrichment. The methane enrichment performance of polymeric membranes is constrained, hence newer material combinations have been investigated to enhance membrane performance. In this study, blend membranes comprised of polyetherimide (PEI) and polyvinyl acetate (PVAc) in varying proportions were prepared by adopting the wet-phase inversion technique. The generated pure, and blend membranes were characterized for the morphological, thermal, and structural study. The interactions of PEI and PVAc in blend samples were verified by FTIR analysis. On the other hand, SEM investigation indicated that the membranes have an anisotropic porous structure with a dense skin layer at the top. Subsequently, a single glass transition temperature (T g ), as validated by DSC analysis, indicates that the blended polymers are miscible. Furthermore, membranes' performance for gas separation was assessed regarding selectivity and permeance at feed pressures ranging from 2 to 6 bar. The permeation results showed that the CO 2 permeance has increased by 40.47% with the addition of 4 wt % PVAc at 2 bar pressure. Furthermore, ideal selectivity improves as the blend ratio increases; nonetheless, the highest value for CO 2 /CH 4 ideal selectivity was attained with a 2 wt % PVAc addition and at 2 bar pressure, which is approximately 26% greater than the pure PEI membrane. At 4 bar pressure, optimum CO 2 /N 2 selectivity value of 22.50 was achieved. The findings indicate that PVAc is an excellent option for expanding the separation performance of blended polymeric membranes for biogas enrichment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This work aims to assess the potential biogas resource of by-products from the production of distilled spirits at 9 operational distilleries in 7 countries. An additional objective was the calculation of the energy resource and Scope 1 greenhouse gas (GHG) emission savings from the use of 21 by-products from the distilleries as a feedstock for anaerobic digestion (AD). To present a holistic perspective on the integration of AD with distilleries, an overview of additional criteria to be considered was provided. The biochemical methane potential (BMP) of the by-products associated with a selection of distilled spirits was experimentally determined. The BMP ranged from 161 L methane per kg volatile solid (LCH 4 /kgVS) to 589 LCH 4 /kgVS with an average value of 332 LCH 4 /kgVS. Biogas could reduce distillery fossil fuel demand by 49% when produced from un-processed by-products, by 66% when produced from a mixture of separated by-products, by 16% when produced from concentrated by-products and by 13% when produced from liquid by-products. The average Scope 1 GHG emission saving when using un-processed by-products was 52%, a mix of separated by-products allowed for a reduction of 66%, liquid by-products achieved an average reduction of 14%, and the use of concentrated by-products reduced GHG emissions by 17% on average. When evaluating which distilleries are "of most interest" for the integration of AD, other criteria to be considered include: by-product properties, the size of the AD facility required, the quantity of digestate produced, and the location of the distilleries in terms of both land availability to construct the AD facility and the proximity to land on which to spread digestate., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Biowaste generation is considerably increasing multiple times recently due to various social and environmental changes like population growth, economic prosperity, globalisation etc. they contain different composition and generated at different stages of their life cycle. Though studies reported for recycle, reproduce and reuse of them, this investigation is unique by focussing to investigate the ideal circumstances for the production of biogas and methane from anaerobic digestion of vegetable waste using response surface methods and artificial neural networks with thermophilic temperature range. Thermophilic temperature of 20.78, organic loading rate of 0.2, pH of 8.81, agitation time of 5.8 and hydro retention time of 3 are the ideal input parameter values for the generation of biogas 3.03 m3 and methane% 186.08 with a desirability of 1. The Response surface model was surpassed by the Artificial Neural Network model., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

With the continuous rise of food waste (FW) throughout the world, a research effort to reveal its potential for bioenergy production is surging. There is a lack of harmonized information and publications available that evaluate the state-of-advance for FW-derived methane production process, particularly from an engineering and sustainability point of view. Anaerobic digestion (AD) has shown remarkable efficiency in the bioconversion of FW to methane. This paper reviews the current research progress, gaps, and prospects in pre-AD, AD, and post-AD processes of FW-derived methane production. Briefly, the review highlights innovative FW collection and optimization routes such as AI that enable efficient FW valorization processes. As weather changes and the FW sources may affect the AD efficiency, it is important to assess the spatio-seasonal variations and microphysical properties of the FW to be valorized. In that case, developing weather-resistant bioreactors and cost-effective mechanisms to modify the raw substrate morphology is necessary. An AI-guided reactor could have high performance when the internal environment of the centralized operation is monitored in real-time and not susceptible to changes in FW variety. Monitoring solvent degradation and fugitive gases during biogas purification is a challenging task, especially for large-scale plants. Furthermore, this review links scientific evidence in the field with full-scale case studies from different countries. It also highlights the potential contribution of ADFW to carbon neutrality efforts. Regarding future research needs, in addition to the smart collection scheme, attention should be paid to the management and utilization of FW impurities, to ensure sustainable AD operations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Lignocellulosic biomass is considered a key resource for the future expansion of biogas production through anaerobic digestion (AD), and research on the development of pretreatment technologies for improving biomass conversion is an intensive and fast-growing field. Consequently, there is a need for creating tools able to predict the efficiency of a certain pretreatment on different biomass types, fast and accurately, and to assist in selecting a pretreatment technology for a specific biomass. In this study, seven different types of raw lignocellulosic biomass of industrial relevance were systematically analyzed regarding their composition (carbohydrates, lignin, lipids, ash, extractives, etc.) and subjected to a common pretreatment. The aim of the study was to identify the most important characteristics that make a biomass good receptor of the specific pretreatment prior to AD. A simple ammonia pretreatment was chosen as a case study and partial least squares regression (PLS-R) was used for modeling initially the ultimate methane yield of raw and pretreated biomass. In the sequel, PLS-R was used for modeling the efficiency of the pretreatment on increasing the ultimate methane yield and hydrolysis rate as a function of the biomass composition. The fit of the models was satisfactory, ranging from R 2  = 0.89 to R 2  = 0.97. The results showed that the most decisive characteristics for predicting the efficiency of the pretreatment were the lipid (r = -0.88), ash (r = +0.79), protein (r = -0.61), and hemicellulose/lignin (r = -0.53) content of raw biomass. Finally, the approach followed in this study facilitated an improved understanding of the mechanism of the pretreatment and presented a methodology to be followed for developing tools for the prediction of pretreatment efficiency in the field of lignocellulosic biomass valorization., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Research within biological methanation has been a great development using biotrickling filters (BTF), as a power-to-x solution, but research within up scaling is missing. This study investigates the commercial potential of biomethanation in BTF by operating two 1 m 3 reactors which was implemented into a full-scale biogas plant. Several areas were investigated, such as enrichment and start-up, long-term steady state operation, serial operation, and intermittent feed. A methane productivity of [Formula: see text] with a product gas of 95.7 % CH 4 was obtained for parallel operation, whereas during serial operation a methane productivity of [Formula: see text] at 97.4 % CH 4 was achieved. The flexibility of the biomethanation was demonstrated with unintentional loss of H 2 feed in periods of 12 to 72 h, where initial performance was regained within 6 to 12 h. The results from this study demonstrate the potential for commercial use of biomethanation in BTF for future Power-to-X solutions., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Anaerobic digestion is a complex process, involving various microorganism groups and, consequently, several reactions. An easy-to-use protocol for the rate-limiting step determination of the process is proposed. The hydrogen production, acetate production, and acetate consumption rates can be calculated, according to a structured algorithm. During the rate limiting step determination, several compounds (biopolymer and monomer representatives, as well as sodium acetate) were used, combined or not with the substrate, to draw the corresponding conclusions. Three substrates were tested, characterized by specific organic compound groups (carbohydrates, proteins, and fats). All three substrates followed the acetate-consuming pathway for the organic matter conversion to methane. In this study, the rate-limiting step for the pathway of acetate consumption was acetate production. Determining the rate-limiting step through the proposed protocol can point to the appropriate actions needed to boost methane production, like substrate pretreatment, using an acidogenic reactor, or checking for the presence of inhibitors., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

This study investigated whole-farm methane emissions from five Danish pig farms with different manure management practices and compared measured emission rates to international and national greenhouse gas inventory emission models. Methane emissions were quantified by using the tracer gas dispersion method. Farms were measured between five and eight times throughout a whole year. One of the farms housed sows and weaners (P1) and the others focused on fattening pigs (P2-P5). The farms had different manure treatment practices including biogasification (P3), acidification (P4-P5) and no manure treatment (liquid slurry) (P1-P2). Quantified methane emissions ranged from 0.2 to 20 kg/h and the highest rates were seen at the farms with fattening pigs and with no manure treatment (P2), while the lowest emissions were detected at farms with manure acidification (P4 and P5). Average methane emission factors (EFs), normalised based on livestock units, were 14 ± 6, 18 ± 9, 8 ± 7, 2 ± 1 and 1 ± 1 g/LU/h, for P1, P2, P3, P4 and P5, respectively. Emissions from fattening pig farms with biogasification (P3) and acidification (P4-P5) facilities were 55% and 91-93% lower, respectively, than from farm with no manure treatment (P2). Inventory models underestimated farm-measured methane emissions on average by 51%, across all models and farms, with the Danish model performing the worst (underestimation of 64%). A revision of model parameters related to manure emissions, such as the estimation of volatile solids excreted and methane conversion factor parameters, could improve model output, although more data needs to be collected to strengthen the conclusions. As one of the first studies assessing whole-pig farm emissions, the results showed the potential of the applied measuring method to identify mitigation strategy efficiencies and highlighted the necessity to investigate inventory model accuracy., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Optimising biogas yields from anaerobic digestion of organic wastes is significant to maximum energy recovery in the biodigestion process and has become an important topic of interest. Substrate particle size is an important process parameter in biogas production, and it precedes other pretreatments methods for the majority of the lignocellulose materials. Optimisation of biogas yield using Response Surface Methodology (RSM) was done, and temperature, hydraulic retention time and particle size were considered variables to develop the predictive models. Pretreatment of groundnut shells was investigated using particle size reduction of mechanical pretreatment methods. After pretreatment, 30 samples were digested in a batch digester at mesophilic temperature. The experimental results showed that the temperature, hydraulic retention time and particle size had significant effects of interaction ( p  < 0.05). The optimum experimental and predicted yields are: 44.70 and 42.92 (l N kgoDM) organic dry matter biogas yield, 20.80 and 19.09 (l N /kgFM) fresh mass biogas yield, 24.00 and 22.68 (l N CH 4 oDM) organic dry methane yield and 12.30 and 15.59 (l N CH 4 FM) fresh mass methane yield, respectively. The R recorded for the four yield components were 0.6268, 0.5875, 0.6109 and 0.5547. These values seem to be lower and a sign of the average fit of the model. Biogas production from groundnut shells was significantly improved with statistical optimisation and the pretreatment method.2 recorded for the four yield components were 0.6268, 0.5875, 0.6109 and 0.5547. These values seem to be lower and a sign of the average fit of the model. Biogas production from groundnut shells was significantly improved with statistical optimisation and the pretreatment method.

Anaerobic fungi (Neocallimastigomycota) are promising lignocellulose-degrading microorganisms that can be exploited by the biofuel industry. While natural production of ethanol by these microorganisms is very low, there is a greater potential for their use in the biogas industry. More specifically, anaerobic fungi can contribute to biogas production by either releasing holocellulose or reducing sugars from lignocelluloses that can be used as a substrate by bacteria and methanogens involved in the anaerobic digestion (AD) process or by metabolizing acetate and formate that can be directly consumed by methanogens. Despite their great potential, the appropriate tools for engineering anaerobic fungi have not been established yet. The first section of this review justifies how the biofuel industry can benefit from using anaerobic fungi and is followed by their taxonomy. In the third section, the possibility of using anaerobic fungi for the consolidated production of bioethanol is briefly discussed. Nevertheless, the main focus of this review is on the upstream and mainstream effects of bioaugmentation with anaerobic fungi on the AD process. The present review also scrutinizes the constraints on the way of efficient engineering of anaerobic rumen fungi. By providing this knowledge, this review aims to help research in this field with identifying the challenges that must be addressed by future experiments to achieve the full potentials of these promising microorganisms. To sum up, the pretreatment of lignocelluloses by anaerobic fungi can prevent carbohydrate loss due to respiration (compared to white-rot fungi). Following fungal mixed acid fermentation, the obtained slurry containing sugars and more susceptible holocellulose can be directly consumed by AD microorganisms (bacteria, methanogens). The bioaugmentation of anaerobic fungi into the AD process can increase methane biosynthesis by >3.3 times. Despite this, for the commercial AD process, novel genetic engineering techniques and kits must be developed to efficiently improve anaerobic fungi viability throughout the AD process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Marine biomass is a promising renewable energy source, especially as this waste contains a large amount of cellulose and hemicellulose, which can contribute to convert it into energy products using anaerobic digestion (AD) and pyrolysis processes. This work was focused on a synergetic view of marine coastal waste treatment (seaweed) using two different technologies, anaerobic microbiological co-digestion, and pyrolysis. The experiments were performed with two merged technologies to assess the captured energy from the digestate in case it is contaminated. Anaerobic co-digestion was conducted using a periodic load laboratory bench with a vertical biogas digester. An evaluation of possible product yields and composition during pyrolysis at a laboratory-scale bench has been performed. The products obtained after the thermal treatment analyzed using an online gas measurement system and gas chromatographs Agilent 7890A with TCD detector (for gases) and Agilent 7890A with MS detector (for liquids).The results demonstrated that biogas yield was 174.1 l/kg (DM). Seaweed washed by seawater yields a higher amount of biogas (202.5 l/kg). Meanwhile, seaweed, sewage sludge, and digestate samples subjected to thermal treatment produced 17%, 30%, and 15% of liquids products, respectively. The economic performance assessment showed that the application of the developed merged approach on an industrial scale could provide an economic return of up to 8.3 $/100 kg of waste. Based on that, merged AD and pyrolysis technologies could be adapted as a promising technology to valorize seaweed wastes and utilize them as a new sustainable source for renewable energy.

Lignocellulosic by-products from agricultural crops represent an important raw material for anaerobic digestion and clean renewable, which is a key component of the circular economy. Lignocellulose is recalcitrant to biodegradation and pretreatments are required to increase methane yield during anaerobic digestion. In this work, the efficacy of different physicochemical pretreatments was compared using corn stover biomass as substrate. Anaerobic digestion of untreated and pretreated corn stover was performed in batch mode at mesophilic temperature (38°C) and organic matter solubilization of pretreated substrates was also investigated. The highest organic matter solubilization occurred in autoclave pretreatment (soluble chemical oxygen demand = 5630 ± 42 mg O 2  L -1 ). However, the highest methane yield was obtained using alkaline pretreatment (367 ± 35 mL CH 4  g -1  VS added ). Alkaline pretreatment increased methane yield by 43.3% compared to untreated control (256 ± 15 mL CH 4  g -1  VS added ). Two mathematical models (i.e. first-order kinetics and transfer function) were utilized to fit the experimental data with the aim of assessing anaerobic biodegradation and to obtain the kinetic constants in all cases studied. Both models adequately fit the experimental results. The kinetic constant, k , of the first-order model increased by 92.8% when stover was pretreated with sulphuric acid compared with control. The transfer function model revealed that the maximum methane production rate, R m , was obtained for the sulphuric acid treatment, which was 63.5% higher compared to control.

Abundant natural gas reserves, along with increased biogas production, have prompted recent interest in harnessing methane as an industrial feedstock for the production of liquid fuels and chemicals. Methane can either be used directly for fermentation or first oxidized to methanol via biological or chemical means. Methanol is advantageous due to its liquid state under normal conditions. Methylotrophy, defined as the ability of microorganisms to utilize reduced one-carbon compounds like methane and methanol as sole carbon and energy sources for growth, is widespread in bacterial communities. However, native methylotrophs lack the extensive and well-characterized synthetic biology toolbox of platform microorganisms like Escherichia coli, which results in slow and inefficient design-build-test cycles. If a heterologous production pathway can be engineered, the slow growth and uptake rates of native methylotrophs generally limit their industrial potential. Therefore, much focus has been placed on engineering synthetic methylotrophs, or non-methylotrophic platform microorganisms, like E. coli, that have been engineered with synthetic methanol utilization pathways. These platform hosts allow for rapid design-build-test cycles and are well-suited for industrial application at the current time. In this review, recent progress made toward synthetic methylotrophy (including methanotrophy) is discussed. Specifically, the importance of amino acid metabolism and alternative one-carbon assimilation pathways are detailed. A recent study that has achieved methane bioconversion to liquid chemicals in a synthetic E. coli methanotroph is also briefly discussed. We also discuss strategies for the way forward in order to realize the industrial potential of synthetic methanotrophs and methylotrophs., (Copyright © 2021 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)

Anaerobic digestion is a promising method for energy recovery through conversion of organic waste to biogas and other industrial valuables. However, to tap the full potential of anaerobic digestion, deciphering the microbial metabolic pathway activities and their underlying bioenergetics is required. In addition, the behavior of organisms in consortia along with the analytical abilities to kinetically measure their metabolic interactions will allow rational optimization of the process. This review aims to explore the metabolic bottlenecks of the microbial communities adopting latest advances of profiling and 13 C tracer-based analysis using state of the art analytical platforms (GC, GC-MS, LC-MS, NMR). The review summarizes the phases of anaerobic digestion, the role of microbial communities, key process parameters of significance, syntrophic microbial interactions and the bottlenecks that are critical for optimal bioenergetics and enhanced production of valuables. Considerations into the designing of efficient synthetic microbial communities as well as the latest advances in capturing their metabolic cross talk will be highlighted. The review further explores how the presence of additives and inhibiting factors affect the metabolic pathways. The critical insight into the reaction mechanism covered in this review may be helpful to optimize and upgrade the anaerobic digestion system., (Copyright © 2021 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)

Anaerobic digestion (AD) is a bioprocess technology that integrates into circular economy systems, which produce renewable energy and biofertilizer whilst reducing greenhouse gas emissions. However, improvements in biogas production efficiency are needed in dealing with lignocellulosic biomass. The state-of-the-art of AD technology is discussed, with emphasis on feedstock digestibility and operational difficulty. Solutions to these challenges including for pre-treatment and bioaugmentation are reviewed. This article proposes an innovative integrated system combining alkali pre-treatment, temperature-phased AD and bioaugmentation techniques. The integrated system as modelled has a targeted potential to achieve a biodegradability index of 90% while increasing methane production by 47% compared to conventional AD. The methane productivity may also be improved by a target reduction in retention time from 30 to 20 days. This, if realized has the potential to lower energy production cost and the levelized cost of abatement to facilitate an increased resource of sustainable commercially viable biomethane., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

As one of the most abundant biomass resources, crop stalks are great potential feedstock available for anaerobic digestion (AD) to produce biogas. However, the specific physical properties and complex chemical structures of crop stalks form strong barriers to efficient AD bioconversion. To overcome these problems, many efforts have been made over the past few years. This paper reviewed recent research in the evolving field of anaerobic bioconversion of crop stalks and was focused on three critical aspects affecting AD performance: various pretreatment methods and their effects on the improvement of crop stalk biodegradability, determination of specific AD operation parameters for crop stalks, and development of AD technologies. Finally, recommendations on the future development of crop stalk AD were proposed., (Copyright © 2021. Published by Elsevier Ltd.)

An innovative process aiming to combine storage and alkali pretreatment of cover crops was investigated using lime as a low cost and environmental friendly reactant. Different lime loadings and Total Solid concentrations (TS) allowed to highlight the abiotic mechanisms of deacetylation during the early stages of the process. Long-term storage experiments of rye and sunflower cover crops at 100 g.kgTS -1 lime loading allowed to evaluate the fermentation kinetics and to compare performances in dry and wet conditions to classical silage storage. The dry condition allowed an efficient alkaline storage and up to a 15.7% Biochemical Methane Potential (BMP) increase, while the wet condition underwent a succession of fermentations with a high butyric acid accumulation and H 2 production, leading to a 13% BMP loss. Silage experiments allowed an efficient preservation of the BMP, with no significant variation over the 6-month storage duration., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

High energy consumption and depletion of fossil fuels lead to the introduction of new technologies to produce alternative fuels with fewer emissions of greenhouse gases. The present investigation was focused to utilize the waste coal washery rejects as a substrate to produce biogenic methane under optimum conditions. Experiments were performed to explore the efficiency of non-coal samples (cow dung, distillery anaerobic digester sludge) and coal mines enriched samples in the degradation of coal washery rejects. Further cow dung, distillery anaerobic sludge, and coal washery rejects were taken at various concentrations to develop anaerobic slurry and analysed for its biogas production. The anaerobic slurry which contains 1:1:1 of cow dung, distillery anaerobic sludge, and coal washery rejects produced methane of around 55.7%. The coal enriched samples showed a maximum of 22.6% of methane. Subsequently, the best methane-producing anaerobic non-coal consortiums were compared with coal enriched microbial culture in converting coal washery rejects of 10 g/l to methane. Results revealed that cow dung inoculum and coal mine enriched inoculum source produced the nearly same amount of methane. This study suggested that the selected anaerobic slurries and coal enriched samples can utilize sub-bituminous coal washery rejects in methane production. Thus, these consortiums can be applied in converting a large amount of coal washery rejects into methane thus can lead to the reclamation of the site., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Anaerobic digestion is a promising method for energy recovery through conversion of organic waste to biogas and other industrial valuables. However, to tap the full potential of anaerobic digestion, deciphering the microbial metabolic pathway activities and their underlying bioenergetics is required. In addition, the behavior of organisms in consortia along with the analytical abilities to kinetically measure their metabolic interactions will allow rational optimization of the process. This review aims to explore the metabolic bottlenecks of the microbial communities adopting latest advances of profiling and 13 C tracer-based analysis using state of the art analytical platforms (GC, GC-MS, LC-MS, NMR). The review summarizes the phases of anaerobic digestion, the role of microbial communities, key process parameters of significance, syntrophic microbial interactions and the bottlenecks that are critical for optimal bioenergetics and enhanced production of valuables. Considerations into the designing of efficient synthetic microbial communities as well as the latest advances in capturing their metabolic cross talk will be highlighted. The review further explores how the presence of additives and inhibiting factors affect the metabolic pathways. The critical insight into the reaction mechanism covered in this review may be helpful to optimize and upgrade the anaerobic digestion system., (Copyright © 2021 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)

Anaerobic codigestion (AnCoD) has attracted attention owing to its advantages over conventional anaerobic digestion, and attempts are still going on to develop methods for improving the efficiency of this technology. Mostly, addition of cosubstrates without applying a proper pretreatment cannot adequately enhance the performance of the digestion. However, there is a lack of a comprehensive study on different pretreatment methods specific to the wide range of cosubstrates. This review aimed to (i) categorize pretreatment techniques that have been developed for improving AnCoD, (ii) present the results of the studies on the effect of pretreatment on improving AnCoD, and (iii) provide a comparison between pretreatment methods and their application for different types of cosubstrates. The findings primarily validated the influence of pretreatment to enhance the process by increasing biodegradability, improved hydrolysis, reduced hydraulic retention time (HRT), and improved methane production. The five main categories of pretreatment employed in codigestion included the following: mechanical, thermal, chemical, biological, and hybrid pretreatment. Among them, mechanical and biological pretreatment have the most and least application in codigestion, respectively. Greater efforts are required on the application of biological pretreatment and cost-benefit analysis of different pretreatment options on the variety of the cosubstrates. PRACTITIONER POINTS: Pretreatment can significantly enhance biomethane production in anaerobic digestion Anaerobic codigestion along with pretreatment can further enhance the conventional anaerobic digestion of single feedstock Mechanical and biological methods have been the most and least practiced pretreatment options Selection of applicable pretreatment option to enhance methane production is subject to the type of cosubstrates in the system There is a research gap in evaluating the application of biological pretreatment for various types of cosubstrates., (© 2021 Water Environment Federation.)

Computational fluid dynamics (CFD) is numerical strategy developed for simulating the behavior of liquid and gas flow. CFD may be applied starting from aerospace, engine design, vehicle aerodynamics, power plants and chemical industries for analyzing and solving relevant system design and process issues. Biogas produced during anaerobic digestion (AD) is sustainable and renewable alternative to fossil fuels. AD may improve the controlled production of biogas and offers significant environmental benefits. This review focuses on research outcomes relevant for enhanced biogas production by exploring the possible applications of CFD in AD technology. CFD-related research performed in AD conditions in order to improve mixing performance, reduce power consumption, and understand the effects of total solid (TS) concentrations on flow behavior have been discussed. In addition, the use of AD for bio-hydrogen production, wastewater treatment, and sludge treatment are looked in. This review also identifies novel areas for AD technology advancement where there is potential for economic improvement in renewable energy production. Finally, future research needs have been identified, focusing on the opportunities to integrate conceptual and mathematical models for advancing CFD simulations for bioenergy.

This study investigated acid splitting wastewater (ASW) and interphase (IF) from soapstock splitting, as well as matter organic non glycerol (MONG) from glycerol processing, as potential substrates for biogas production. Batch and semicontinuous thermophilic anaerobic digestion experiments were conducted, and the substrates were preliminary treated using commercial enzymes kindly delivered by Novozymes A/C. The greatest enhancement in the batch digestion efficiency was achieved when three preparations; EversaTransform, NovoShape, and Lecitase were applied in the hydrolysis stage, which resulted in the maximum methane yields of 937 NL/kg VS and 915 NL/kg VS obtained from IF and MONG, respectively. The co-digestion of 68% ASW, 16% IF, and 16% MONG (wet weight basis) performed at an organic loading rate (OLR) of 1.5 kg VS/m 3 /day provided an average methane yield of 515 NLCH 4 /kg VS added and a volatile solid reduction of nearly 95%. A relatively high concentration of sulfates in the feed did not significantly affect the digestion performance but resulted in an increased hydrogen sulfide concentration in the biogas with the peak of 4000 ppm.

To develop biotechnological process for methane to methanol conversion, selection of a suitable methanotrophic platform is an important aspect. Systematic approach based on literature and public databases was developed to select representative methanotrophs Methylotuvimicrobium alcaliphilum, Methylomonas methanica, Methylosinus trichosporium and Methylocella silvestris. Selected methanotrophs were further investigated for methanol tolerance and methanol production on pure methane as well as biogas along with key enzyme activities involved in methane utilization. Among selected methanotrophs M. alcaliphilum showed maximum methanol tolerance of 6% v/v along with maximum methanol production of 307.90 mg/L and 247.37 mg/L on pure methane and biogas respectively. Activity of methane monooxygenase and formate dehydrogenase enzymes in M.alcaliphilum was significantly higher up to 98.40 nmol/min/mg cells and 0.87 U/mg protein, respectively. Biotransformation trials in 14 L fermentor resulted in increased methanol production up to 418 and 331.20 mg/L, with yield coefficient 0.83 and 0.71 mg methanol/mg of pure methane and biogas respectively. The systematic selection resulted in haloalkaliphilic strain M. alcaliphilum as one of the potential methanotroph for bio-methanol production., (Copyright © 2021 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

Current anaerobic digestion (AD) design methods rely on crude empirical models or sophisticated anaerobic digestion models (like ADM1) requiring a large number of parameters which are difficult to obtain experimentally. A simplified model for simulating AD was developed in this work. The model requires knowledge of CH 4 /CO 2 ratio in biogas or indigestible fraction in substrate and batch biomethane potential (BMP) data for estimating three kinetic parameters (maximum specific growth rate, half velocity constant and cell death rate). Reported lab scale BMP data of sugarcane bagasse and spent wash were used to first estimate the kinetics and then to simulate corresponding largescale AD. Simulated results of specific methane yield and digester performance were consistent with available largescale AD data. The potential of the model to simulate single and multi-stage AD were illustrated. The presented approach and model will be useful for effectively valorising a variety of complex biomass substrates to biogas., (Copyright © 2021. Published by Elsevier Ltd.)

The need for a more sustainable wastewater treatment is more relevant now due to climate change. Production and reuse of methane from anaerobic treatment is one pathway. However, this is defeated by the presence of dissolved methane in the effluent and would be released to the environment, adding to the greenhouse gas emissions. This review paper provided summary and analysis of studies involved in the production of dissolved methane from AnMBR, focusing with actual methane measurement (gas and dissolved) from AnMBR with different types of wastewater. Then more focused discussion and analysis on the use of membrane-based technology or membrane contactors in the recovery of dissolved methane from AnMBR effluent are included, with its development and energy analysis. The dissolved methane removal and recovery rate of membrane contactors can be as high as 96% and 0.05 mol methane/m 2 /h, respectively, with very low additional energy requirement of 0.01 kWh/m 3 for the recovery. Future perspectives presented focus on the long-term evaluation and modelling of membrane contactors and on the membrane modifications to improve the selectivity of membranes to methane and to limit their fouling and wetting, thus making the technology more economical for resource recovery., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) enzyme found in plants, algae, and an array of autotrophic bacteria is also encoded by a subset of methanotrophs, but its role in these microbes has largely remained elusive. In this study, we showed that CO 2 was requisite for RubisCO-encoding Methylococcus capsulatus strain Bath growth in a bioreactor with continuous influent and effluent gas flow. RNA sequencing identified active transcription of several carboxylating enzymes, including key enzymes of the Calvin and serine cycles, that could mediate CO 2 assimilation during cultivation with both CH 4 and CO 2 as carbon sources. Marker exchange mutagenesis of M. capsulatus Bath genes encoding key enzymes of potential CO 2 -assimilating metabolic pathways indicated that a complete serine cycle is not required, whereas RubisCO is essential for growth of this bacterium. 13 CO 2 tracer analysis showed that CH 4 and CO 2 enter overlapping anaplerotic pathways and implicated RubisCO as the primary enzyme mediating CO 2 assimilation in M. capsulatus Bath. Notably, we quantified the relative abundance of 3-phosphoglycerate and ribulose-1,5-bisphosphate 13 C isotopes, which supported that RubisCO-produced 3-phosphoglycerate is primarily converted to ribulose-1-5-bisphosphate via the oxidative pentose phosphate pathway in M. capsulatus Bath. Collectively, our data establish that RubisCO and CO 2 play essential roles in M. capsulatus Bath metabolism. This study expands the known capacity of methanotrophs to fix CO 2 via RubisCO, which may play a more pivotal role in the Earth's biogeochemical carbon cycling and greenhouse gas regulation than previously recognized. Further, M. capsulatus Bath and other CO 2 -assimilating methanotrophs represent excellent candidates for use in the bioconversion of biogas waste streams that consist of both CH 4 and CO 2 . IMPORTANCE The importance of RubisCO and CO 2 in M. capsulatus Bath metabolism is unclear. In this study, we demonstrated that both CO 2 and RubisCO are essential for M. capsulatus Bath growth. 13 CO 2 tracing experiments supported that RubisCO mediates CO 2 fixation and that a noncanonical Calvin cycle is active in this organism. Our study provides insights into the expanding knowledge of methanotroph metabolism and implicates dually CH 4 /CO 2 -utilizing bacteria as more important players in the biogeochemical carbon cycle than previously appreciated. In addition, M. capsulatus and other methanotrophs with CO 2 assimilation capacity represent candidate organisms for the development of biotechnologies to mitigate the two most abundant greenhouse gases, CH 4 and CO 2 .

Wastewater treatment plants and other remediation facilities serve important roles, both in public health, but also as dynamic research platforms for acquiring useful resources and biomolecules for various applications. An example of this is methanotrophic bacteria within anaerobic digestion processes in wastewater treatment plants. These bacteria are an important microbial source of many products including ectoine, polyhydroxyalkanoates, and methanobactins, which are invaluable to the fields of biotechnology and biomedicine. Here we provide an overview of the methanotrophs' unique metabolism and the biochemical pathways involved in biomolecule formation. We also discuss the potential biomedical applications of these biomolecules through creation of beneficial biocompatible products including vaccines, prosthetics, electronic devices, drug carriers, and heart stents. We highlight the links between molecular biology, public health, and environmental science in the advancement of biomedical research and industrial applications using methanotrophic bacteria in wastewater treatment systems.

Solid state anaerobic digestion (SSAD) of lignocellulosic biomass may be attractive solution for its valorisation. Compared to liquid state anaerobic digestion (LSAD), SSAD can handle higher organic loading rates (OLR), requires a less water and smaller reactor volume. It may require lower energy demand for heating or mixing and has higher volumetric methane productivity. Besides numerous benefits of SSAD processes and progress in system design, there are still obstacles, which need to be overcome for its successful implementations. This review aims to compile the recent trends in enhancing the bioconversion of agricultural stubbles in SSAD. Several pretreatment procedures used to breaking lignin and cellulose complex, method to overcome carbon to nitrogen ratio imbalance, use of carbon-based conducting materials to enhance Volatile Fatty Acids (VFA) conversion and additives for achieving nutrient balance will be discussed in this review. Leachate recirculation and its impacts on SSAD of agricultural stubbles are also discussed., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

This investigation evaluates a two-stage process to treat highly concentrated winery effluents, including a thermophilic methanogenic stage. The inoculum adaptation, the effect of the organic loading rates on both stages, and the methanogenic reactor's feeding frequency on the process performance were studied. An active thermophilic inoculum was obtained by a one-step temperature increase from 35 to 55 °C. The application of organic loads above 120 kg COD m -3 d -1 in the acidogenic stage ensured the highest acetic acid concentration, while methane production rates as high as 7.1 Nm 3 CH 4 m -3 d -1 and a yield of 348 L CH 4 kg -1 COD were obtained in the thermophilic methanogenic stage using an organic loading rate of 29.9 kg COD m -3 d -1 . However, a lower removal of organic matter was observed under that condition. Lower feeding frequencies improved methane productivity and yield, suggesting that this parameter is a useful process optimization tool., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The aim of this work was to study an innovative alkaline process on two cover crops. CaO load of 60 g.kgTS -1 was implemented to combine the functions of storage and pretreatment. Lab-scale reactors were monitored for 180 days to assess the effect of this process on the physico-chemical properties of the biomass. From the first days, pH was not maintained in an alkaline zone and microbial fermentation activity was observed with the degradation of available carbohydrates and production of metabolites, CO 2 and H 2 . High butyric acid accumulation was observed and mass losses of 18.1% and 9.0% of initial VS occurred for oat and rye, respectively. However, no methane potential loss was recorded in the short and long term and the crops were efficiently preserved. The pretreatment had no major impact on fiber solubilization, and no increase in BMP was obtained, which was attributed to the short duration of the alkaline conditions., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

World's energy consumption has doubled in the last four decades, this demand has been covered by the usage of fossil fuels; which are an important source of greenhouse gases emissions, entailing climate change. An efficient less polluting management alternative is the anaerobic digestion (AD) process which generates biogas derived from organic matter. The methane contained at the biogas is considered a biofuel that can generate electricity and heat through its combustion. Biochemical Methane Potential (BMP) essays are standardized methodologies carried out to estimate the energy potential of an organic residue. After performing a BMP test for 13 residual biomass samples, it can be concluded that fresh livestock rumen and manure were the best substrates to generate higher biogas yields; ranging from 1119-1669 ml/g VS added , with high methane content (65%4 <77%) doubling the results reported at literature (≈550 ml biogas/g VS added ). Results indicate that energy potential of these residual biomasses can be increased 23-25% by improving management (freshness) and environmental (higher temperature) conditions and, by reducing the nutrimental deficiencies (mineral medium) of microorganisms. Fresh swine manure also produced large volumes of biogas (1150 ml biogas/g VS added ); however, average methane content ranged 50-65% CH 4 v/v. The rest of the residual biomasses produced biogas and methane yields within the normal ranges (≈550 ml biogas/g VS added ); meaning that also can be used as substrate for bioenergy generation. Residues from aquaculture and fish based-industry produced low quantities of biogas (<250 ml biogas/g VS added ; CH4 < 20%); therefore, these cannot be recommended as substrates for bioenergy purposes.

Global methane emissions have been steadily increasing over the past few decades, exerting a negative effect on the environment. Biogas from landfills and sewage treatment plants is the main anthropogenic source of methane. This makes methane bioconversion one of the priority areas of biotechnology. This process involves the production of biochemical compounds from non-food sources through microbiological synthesis. Methanotrophic bacteria are a promising tool for methane bioconversion due to their ability to use this greenhouse gas and to produce protein-rich biomass, as well as a broad range of useful organic compounds. Currently, methane is used not only to produce biomass and chemical compounds, but also to increase the efficiency of water and solid waste treatment. However, the use of gaseous substrates in biotechnological processes is associated with some difficulties. The low solubility of methane in water is one of the major problems. Different approaches have been involved to encounter these challenges, including different bioreactor and gas distribution designs, solid carriers and bulk sorbents, as well as varying air/oxygen supply, the ratio of volumetric flow rate of gas mixture to its consumption rate, etc. The aim of this review was to summarize the current data on different bioreactor designs and the aspects of their applications for methane bioconversion and wastewater treatment. The bioreactors used in these processes must meet a number of requirements such as low methane emission, improved gas exchange surface, and controlled substrate supply to the reaction zone., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Hydrogenotrophic biomethanation in a biotrickling filter has been reported to be a proven technology for biological biogas upgrading in recent studies. However, the preparation of enriched hydrogenotrophic methanogens in a separate reactor prior to biomethanation in a trickled bed is a lengthy procedure and therefore hard to apply on an industrial scale. This study explored the direct inoculation of anaerobic biogas digestate for simultaneous enrichment of hydrogenotrophic methanogens and biofilm immobilisation in a trickled bed system. The direct inoculation and formation of hydrogenotrophic biofilm was successful and resulted in a stable H 2 loading rate of 11  [Formula: see text] , with the highest specific methane productivity recorded at 3.03 Nm 3 m R -3 d -1 and a purity of 98% CH 4 in thermophilic conditions. The DNA analysis confirmed that hydrogenotrophic methanogens dominated the archaeal consortia., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Stimulating direct interspecies electron transfer with conductive materials is a promising strategy to overcome the limitation of electron transfer efficiency in syntrophic methanogenesis of industrial wastewater. This paper assessed the impact of conductive foam nickel (FN) supplementation on syntrophic methanogenesis and found that addition of 2.45 g/L FN in anaerobic digestion increased the maximum methane production rate by 27.4 % (on day 3) while decreasing the peak production time by 33 % as compared to the control with no FN. Cumulative methane production from day 2 to 6 was 14.5 % higher with addition of 2.45 g/L FN than in the control. Levels of FN in excess of 2.45 g/L did not show benefits. Cyclic voltammetry results indicated that the biofilm formed on the FN could generate electrons. The dominant bacterial genera in suspended sludge were Dechlorobacter and Rikenellaceae DMER64, whereas that in the FN biofilm was Clostridium sensu stricto 11. The dominant archaea Methanosaeta in the FN biofilm was enriched by 14.1 % as compared to the control., (Copyright © 2020 Elsevier B.V. All rights reserved.)

In this paper, we present a dynamic simulation tool for biomethane policy design in Latvia. A new System Dynamics based computer simulation model covering the whole biogas-to-biomethane value chain including bioresource availability, biogas production and biogas utilization pathways is proposed. The goal of the model is to evaluate the efficiency of a set of policy instruments on increasing the transition from biogas for electricity production to biogas upgrading to be used as a transportation fuel thus contributing to national renewable energy targets in the transport sector by 2030. According to the modeling results, policy design should be focused on two directions, reducing the existing support mechanisms for electricity producers and subsidizing biomethane production, namely. Additional benefits are brought by tax policies aiming to increase the price of competing fossil fuels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

This study proposes a novel method to directly treat reject water with a high ammonium content, without relying on dilution. The originality of this method resides in leveraging the coordinated action of a methane- and methanol-dependent bacterial consortium and the biogas generated from wastewater treatment facilities. Specifically, ammonium is removed through autotrophic assimilation in the glutamate cycle of methanotrophs and Methylophilus while, simultaneously, methanol generated by methanotrophs is treated through formaldehyde assimilation as Methylophilus undergo the same ribulose monophosphate cycle as methanotrophs. Using this method, the backflow of high-concentration ammonium into the wastewater treatment process was reduced to 59% in a single operation using a sequencing batch reactor at a mean influent concentration of 877.3 mg L -1 . However, the removal rate temporarily declined to an average of 37.6% at a concentration of 800 mg L -1 or above, which was imputed to the influence of toxic intermediates., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The anaerobic digestion (AD) of a high diversity blend of fruit and vegetable waste (FVW) generated in tropical conditions as a single substrate was performed. A continuously stirred tank reactor (CSTR) operated in semi-continuous regime was used for AD. The reactor performance was monitored with gradually increasing organic loading rates (OLRs) from 0.5 up to 5.0 gVS L - 1 . The biochemical methane potential (BMP) of FVW determined by batch bottles was 360 L -1 . The biochemical methane potential (BMP) of FVW determined by batch bottles was 360 L N  CH 4 , with a biodegradability of 79%. A stable pH with an adequate level of buffering capacity was observed during the entire experiment. Methane yield indicated the best performance at an OLR of 3.0 gVS L VS -1 , with a biodegradability of 79%. A stable pH with an adequate level of buffering capacity was observed during the entire experiment. Methane yield indicated the best performance at an OLR of 3.0 gVS L - 1  d -1 , with 285 L N  CH 4 accumulation of volatile fatty acids (VFA) was detected; in particular, propionic acid was monitored, and a decreased methane yield was detected. Biogas production rate was 1.55 L VS -1 added, reaching 79% of BMP. At an OLR over 3.0 gVS L - 1  d -1 accumulation of volatile fatty acids (VFA) was detected; in particular, propionic acid was monitored, and a decreased methane yield was detected. Biogas production rate was 1.55 L N  L - 1  d -1 and showed linear increase according to increases in the OLR.