1. DT-13, a saponin of dwarf lilyturf tuber, exhibits anti-cancer activity by down-regulating C-C chemokine receptor type 5 and vascular endothelial growth factor in MDA-MB-435 cells
- Author
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Lin Sen-Sen, Shengtao Yuan, Luyong Zhang, Bo-Yang Yu, Li Sun, Zhao Ren-Ping, and Xianshu Bai
- Subjects
Vascular Endothelial Growth Factor A ,Receptors, CCR5 ,Cell ,Mice, Nude ,Breast Neoplasms ,Metastasis ,Mice ,chemistry.chemical_compound ,Chemokine receptor ,Cell Movement ,In vivo ,Cell Line, Tumor ,Drug Discovery ,Cell Adhesion ,medicine ,Animals ,Humans ,Liriope Plant ,Traditional medicine ,Chemistry ,Cell growth ,General Medicine ,Saponins ,medicine.disease ,Antineoplastic Agents, Phytogenic ,Molecular biology ,In vitro ,Vascular endothelial growth factor ,Plant Tubers ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,Complementary and alternative medicine ,CCR5 Receptor Antagonists ,Female ,Drugs, Chinese Herbal - Abstract
Aim To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action. Methods MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from Ophiopogon japonicus, in vitro . In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor (VEGF), C-C chemokine receptor type 5 (CCR5) and hypoxia-inducible factor 1 α (HIF-1 α ) were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay. Results At 0.01 to 1 μmol·L −1 , DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro . DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1α. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo . Conclusion DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro , and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-1 α expression.
- Published
- 2014