1. Nuclear magnetic resonance-based metabolomics approach to evaluate preventive and therapeutic effects of Gastrodia elata Blume on chronic atrophic gastritis
- Author
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Ling-Yu Ruan, Yong-Hong Fu, Cheng Chen, Ming-Hui Li, Jian-Feng Chen, Yue-Xiao Xing, Wen-Long Zhao, Wei Hong, Jun-Song Wang, Hui-Hui Meng, and Han Xu
- Subjects
Gastritis, Atrophic ,Male ,Magnetic Resonance Spectroscopy ,Atrophic gastritis ,Clinical Biochemistry ,Anti-Inflammatory Agents ,Pharmaceutical Science ,Pharmacology ,01 natural sciences ,Antioxidants ,Analytical Chemistry ,Gastric Acid ,Rats, Sprague-Dawley ,Metabolomics ,Gastric glands ,Drug Discovery ,Asian country ,medicine ,Animals ,Humans ,Spectroscopy ,Gastrodia ,biology ,Plant Extracts ,010405 organic chemistry ,Chemistry ,Stomach ,010401 analytical chemistry ,Therapeutic effect ,biology.organism_classification ,medicine.disease ,Gastrodia elata ,Rats ,0104 chemical sciences ,Disease Models, Animal ,medicine.anatomical_structure ,Gastric Mucosa ,Purines ,Gastric acid ,Energy Metabolism - Abstract
Chronic atrophic gastritis (CAG) is one of the most common digestive system diseases worldwide which defined by WHO as initial step of cancer. Gastrodia elata Blume (GEB) is a traditional herbal with multiple pharmacological activities which was widely used in Asian countries. This study aims to explore the preventive and therapeutical effects of Gastrodia elata Blume on auto-immune induced CAG in rats. Tissues of stomachs were collected and submitted to 1H NMR-based metabolomics analysis and histopathological inspection. The biochemical indexes of MDA, SOD, GSH, NO and XOD were measured. Gastrodia elata Blume could apparently ameliorate the damaged gastric glands and the biochemical parameters, enhance gastric acid secretion, and significantly relieve the inflammation of the stomach. Orthogonal signal correction-partial least squares-discriminant analysis (OSC-PLS-DA) of NMR profiles and correlation network analysis revealed that Gastrodia elata Blume could effectively treat CAG via regulating energy and purine metabolisms, and by anti-oxidation and anti-inflammation effects.
- Published
- 2019